FlyBase Allele Report: Dmel\Inx2[JF02446]

General Information

Symbol

Dmel\Inx2^JF02446

Species

D. melanogaster

Name

FlyBase ID

FBal0242150

Feature type

allele

Associated gene

Dmel\Inx2

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02446}

Also Known As

JF02446

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02446}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02446

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^Mz709

(Chaturvedi et al., 2014)

abnormal visual behavior, with Scer\GAL4^Mz709

(Chaturvedi et al., 2014)

decreased cell number | adult stage, with Scer\GAL4^tj.PU

(Smendziuk et al., 2015)

decreased occurrence of cell division | adult stage, with Scer\GAL4^tj.PU

(Smendziuk et al., 2015)

increased cell number | adult stage, with Scer\GAL4^tj.PU

(Smendziuk et al., 2015)

lethal - all die before end of larval stage, with Scer\GAL4^repo.PL

(Volkenhoff et al., 2018)

lethal - all die during embryonic stage, with Scer\GAL4^repo

(Chaturvedi et al., 2014)

long lived, with Scer\GAL4^repo.PL

(Volkenhoff et al., 2018)

semi-sterile, with Scer\GAL4^tj.PU

(Smendziuk et al., 2015)

short lived | nutrition conditional, with Scer\GAL4^repo.PL

(Volkenhoff et al., 2018)

viable, with Scer\GAL4^elav-C155

(Chaturvedi et al., 2014)

Phenotype Manifest In

male germline stem cell, with Scer\GAL4^tj.PU

(Smendziuk et al., 2015)

photoreceptor neuron, with Scer\GAL4^Mz709

(Chaturvedi et al., 2014)

somatic cell of testis, with Scer\GAL4^tj.PU

(Smendziuk et al., 2015)

synaptic vesicle, with Scer\GAL4^Mz709

(Chaturvedi et al., 2014)

testis, with Scer\GAL4^tj.PU

(Smendziuk et al., 2015)

Detailed Description

Statement

Reference

Expression of Inx2^JF02446 under the control of Scer\GAL4^ey-OK107 in adults does not significantly affect 3 hour aversive olfactory memory, compared to controls.

(Shyu et al., 2019)

Expression of Inx2^JF02446 under the control of Scer\GAL4^repo.PL (in combination with tub-Gal80[ts] transgene to restrict expression to the adult stage) leads to a significantly reduced life span under sugar only diet when compared to controls.

(Volkenhoff et al., 2018)

Expression of Inx2^JF02446 under the control of Scer\GAL4^tj.PU (along with expression of UAS-Dcr2 to increase RNAi efficiency) results in disruption of spermatogenesis, sterility and small, rudimentary testes. Germline stem cell number in testes is significantly reduced, early somatic cell number is increased, and late somatic cell number is decreased. Somatic cyst stem cell proliferation is disrupted. The number of germline cysts that reach meiotic stages is reduced. Encapsulation of testis germline cysts occurs normally, and cadherin-mediated cell-cell adhesion is unaffected in testes expressing Inx2^JF02446 under the control of Scer\GAL4^tj.PU.

(Smendziuk et al., 2015)

Neuronal expression of Inx2^JF02446 under the control of Scer\GAL4^elav-C155 does not affect fly viability or visual signal transmission.

Expression of Inx2^JF02446 using Scer\GAL4^Mz709 does not cause obvious morphological change to satellite glia. These flies show normal arrays of ommatidia and cartridges in the retina and lamina, respectively. In the lamina, fine structures such as capitate projections and T-bar synapses appear normal in photoreceptor terminals. However, photoreceptor terminals exhibit a reduced number of synaptic vesicles. The density of these synaptic vesicles is approximately 50% of wild-type.

Knockdown of Inx2 through expression of Inx2^JF02446 using Scer\GAL4^Mz709 results in the absence of ON and OFF transients in ERG recordings of the laminar LMC neurons at the onset and end of light stimulation.

(Chaturvedi et al., 2014)

Expression of Inx2^JF02446 under the control of Scer\GAL4^ptc-559.1 has no effect on the wing margin.

(Ayukawa et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Gmd^NIG.8890R, Inx2^JF02446, Scer\GAL4^ptc-559.1 has visible phenotype

(Ayukawa et al., 2012)

Phenotype Manifest In

Other

Statement

Reference

Gmd^NIG.8890R, Inx2^JF02446, Scer\GAL4^ptc-559.1 has wing margin phenotype

(Ayukawa et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Inx2^JF02446 and Inx3^HM05245, both under the control of Scer\GAL4^tj.PU, phenocopies expression of Inx2^JF02446 alone under the control of Scer\GAL4^tj.PU.

(Smendziuk et al., 2015)

Co-expression of Gmd^NIG.8890R and Inx2^JF02446 under the control of Scer\GAL4^ptc-559.1 results in loss of wing margin.

(Ayukawa et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Inx2^JF02446 is partially rescued by Inx2^UAS.EGFP/Scer\GAL4^repo

(Chaturvedi et al., 2014)

Comments

Expression of Inx2^{Scer\UAS.T:Avic\GFP-EGFP} in glia, under the control of Scer\GAL4^repo, partially rescues flies expressing Inx2^JF02446 under the control of Scer\GAL4^repo. These rescued flies show normal visual transmission, excluding a role of neuronal Inx2 in visual transmission.

(Chaturvedi et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29306

y¹ v¹; P{TRiP.JF02446}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Inx2^JF02446

Inx2^TripJF02446

(Volkenhoff et al., 2018)

inx2^JF02446

(Smendziuk et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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