FlyBase Allele Report: Dmel\CycE[JF02473]

General Information

Symbol

Dmel\CycE^JF02473

Species

D. melanogaster

Name

FlyBase ID

FBal0242158

Feature type

allele

Associated gene

Dmel\CycE

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02473}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02473}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02473

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^en-e16E

(Djiane et al., 2013)

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

decreased cell number | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

visible, with Scer\GAL4^salm.EPv

(Barrios et al., 2015)

Phenotype Manifest In

fat cell | third instar larval stage, with Scer\GAL4^Cg.PA

(Sun et al., 2019)

intermediate neuronal progenitor | decreased number | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

larval outer optic anlage, with Scer\GAL4^c768

(Zhou and Luo, 2013)

microtubule | third instar larval stage, with Scer\GAL4^Cg.PA

(Sun et al., 2019)

nucleus | third instar larval stage, with Scer\GAL4^Cg.PA

(Sun et al., 2019)

type II neuroblast | decreased number | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

(Zhang et al., 2024)

wing, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

wing, with Scer\GAL4^salm.EPv

(Barrios et al., 2015)

wing | posterior, with Scer\GAL4^en-e16E

(Djiane et al., 2013)

Detailed Description

Statement

Reference

Expressing CycE^JF02473 under the control of Scer\GAL4^Cg.PA leads to adipocytes exhibiting reduced perinuclear microtubule concentration and decreased ploidy, without preventing differentiation, as they are still full of lipid droplets. Decreased ploidy is also observed upon conditional expression from L2 onwards (temporal control of expression using Gal80[ts]).

(Sun et al., 2019)

The expression of CycE^JF02473 under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) leads to a significant decrease in wing size, as compared to controls.

(Hevia et al., 2017)

Expression of CycE^JF02473 under the control of Scer\GAL4^en.PU (restricted to 16 hours prior the third instar larval stage onwards using Scer\GAL80^ts.αTub84B) has no effect on wing disc cell size.

Expression of CycE^JF02473 under the control of Scer\GAL4^salm.EPv results in wing notching.

(Barrios et al., 2015)

Scer\GAL4^en-e16E-mediated expression results in a strong reduction in size of the posterior compartment of the adult wing.

(Djiane et al., 2013)

Expression of CycE^JF02473 under the control of Scer\GAL4^c768 results defects in maintenance of the neuroepithelium cells in the developing optic lobe and premature neuroblast formation.

(Zhou and Luo, 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

CycE^JF02473, Scer\GAL4^nub.PU is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, Smox^NIG.2262R

(Hevia et al., 2017)

Suppressor of

Statement

Reference

CycE^JF02473, Scer\GAL4^nub.PU is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

CycE^JF02473, Scer\GAL4^ptc-559.1 is a suppressor | partially of hyperplasia phenotype of N^UAS.icd, Scer\GAL4^ptc-559.1

(Djiane et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

CycE^JF02473, Scer\GAL4^nub.PU is an enhancer of wing phenotype of Scer\GAL4^nub.PU, Smox^NIG.2262R

(Hevia et al., 2017)

Suppressor of

Statement

Reference

CycE^JF02473, Scer\GAL4^Cg.PA is a suppressor of fat cell | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^JF02971

(Sun et al., 2019)

CycE^JF02473, Scer\GAL4^Cg.PA is a suppressor of nucleus | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^JF02971

(Sun et al., 2019)

CycE^JF02473, Scer\GAL4^nub.PU is a suppressor of wing phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of CycE^JF02473 enhances the decreased wing size induced by the expression of Smox^NIG.2262R under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi), while enhancing the severely increased wing size induced by the expression of Smox^{SDVD.Scer\UAS} under the control of Scer\GAL4^nub.PU.

(Hevia et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29314

y¹ v¹; P{TRiP.JF02473}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

CycE^JF02473

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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