FlyBase Allele Report: Dmel\Atg5[JF02703]

General Information

Symbol

Dmel\Atg5^JF02703

Species

D. melanogaster

Name

FlyBase ID

FBal0242290

Feature type

allele

Associated gene

Dmel\Atg5

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02703}

Also Known As

UAS-Atg5-RNAi, Atg5 RNAi, UAS-Atg5^RNAi, UAS-Atg5 RNAi

Key Links

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Perkins et al., 2009)

in vitro construct - regulatory fusion

(Perkins et al., 2009)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{TRiP.JF02703}

Cytology

Nature of the lesion

Statement

Reference

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Atg5

Also carries

loxP

recombinase target site

Associated Sequence Features

dsRNA-JF02703

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | P-stage, with Scer\GAL4^NP0001

(Xu et al., 2015)

abnormal starvation stress response | larval stage, with Scer\GAL4^Cg.PU

(Xu et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Nagy et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Atg5^JF02703 under the control of Scer\GAL4^Cg.PA blocks the starvation-induced accumulation of acidic vesicles (LysoTracker-positive) in the early third instar larval fat body, as compared to controls. Under standard conditions, there is no change in the number of autophagosome-like structures (Atg8a-positive), as compared to controls.

(Liu et al., 2018)

The adulthood-only expression of Atg5^JF02703 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to a severe decrease in the numbers of intestinal stem cells and of mitotic figures in the adult midgut epithelium, as compared to controls.

(Nagy et al., 2016)

Expression of Atg5^JF02703 under the control of Scer\GAL4^{Myo31DF-NP0001} results in significantly delayed midgut degradation after pupariation, with increased size of the midgut and gastric caeca 0 hrs and 4 hrs after puparium formation, but no significant difference in autophagy levels 2 hrs after puparium formation, as compared to controls.

Expression of Atg5^JF02703 under the control of Scer\GAL4^cg.PU results in a decrease in starvation-induced autophagy in the larval fat body, as compared to controls.

(Xu et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Atg5^JF02703, Scer\GAL4^NP5130 has decreased cell number | adult stage phenotype, non-suppressible by Uvrag^HMS01357, Scer\GAL4^NP5130

(Nagy et al., 2016)

Atg5^JF02703, Scer\GAL4^NP5130 has decreased occurrence of cell division | adult stage phenotype, non-suppressible by Uvrag^HMS01357, Scer\GAL4^NP5130

(Nagy et al., 2016)

Enhancer of

Statement

Reference

Atg5^JF02703, Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^{dsRNA.Sym.UAS}

(Kwon et al., 2013)

Suppressor of

Statement

Reference

Atg5^JF02703, Scer\GAL4^NP5130 is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

Atg5^JF02703, Scer\GAL4^NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

NOT Suppressor of

Statement

Reference

Atg5^JF02703, Scer\GAL4^Cg.PA is a non-suppressor of decreased cell size | third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Other

Statement

Reference

Atg5^JF02703, Scer\GAL4^Mef2.PR, park^Δ21/park¹, sfl^unspecified has partially lethal phenotype

(Reynolds-Peterson et al., 2020)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Atg5^JF02703, Scer\GAL4^NP5130 has adult midgut epithelium phenotype, non-suppressible by Uvrag^HMS01357, Scer\GAL4^NP5130

(Nagy et al., 2016)

Atg5^JF02703, Scer\GAL4^NP5130 has intestinal stem cell of posterior adult midgut epithelium phenotype, non-suppressible by Uvrag^HMS01357, Scer\GAL4^NP5130

(Nagy et al., 2016)

Enhancer of

Statement

Reference

Atg5^JF02703, Scer\GAL4^bbg-C96 is an enhancer of wing margin phenotype of Scer\GAL4^bbg-C96, dom^{dsRNA.Sym.UAS}

(Kwon et al., 2013)

Suppressor of

Statement

Reference

Atg5^JF02703, Scer\GAL4^Cg.PA is a suppressor | partially of fat cell | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Atg5^JF02703, Scer\GAL4^Cg.PA is a suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Atg5^JF02703, Scer\GAL4^NP5130 is a suppressor of adult midgut epithelium phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

Atg5^JF02703, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Uvrag^HMS01357

(Nagy et al., 2016)

NOT Suppressor of

Statement

Reference

Pink1^UAS.cCa/Atg5^JF02703, Scer\GAL4^Cg.PA is a non-suppressor of fat cell | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Pink1^UAS.cCa/Atg5^JF02703, Scer\GAL4^Cg.PA is a non-suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The adulthood-only co-expression of Uvrag^HMS01357 and Atg5^JF02703 under the control of Scer\GAL4^esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to the same severely decreased numbers of intestinal stem cells and mitotic figures in the adult midgut epithelium as upon the single expression of Atg5^JF02703, rather than the converse increases induced by the single expression of Uvrag^HMS01357.

(Nagy et al., 2016)

The wing nicking phenotype caused by expression of dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96 is enhanced if the flies are also co-expressing Atg5^JF02703.

(Kwon et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27551

y¹ v¹; P{TRiP.JF02703}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ATG5^JF02703

(Kim et al., 2019)

Atg5^JF02703

(Billes et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (16)

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