UAS regulatory sequences drive expression of an inverted repeat.
The expression of Atg5JF02703 under the control of Scer\GAL4Cg.PA blocks the starvation-induced accumulation of acidic vesicles (LysoTracker-positive) in the early third instar larval fat body, as compared to controls. Under standard conditions, there is no change in the number of autophagosome-like structures (Atg8a-positive), as compared to controls.
The adulthood-only expression of Atg5JF02703 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to a severe decrease in the numbers of intestinal stem cells and of mitotic figures in the adult midgut epithelium, as compared to controls.
Expression of Atg5JF02703 under the control of Scer\GAL4Myo31DF-NP0001 results in significantly delayed midgut degradation after pupariation, with increased size of the midgut and gastric caeca 0 hrs and 4 hrs after puparium formation, but no significant difference in autophagy levels 2 hrs after puparium formation, as compared to controls.
Expression of Atg5JF02703 under the control of Scer\GAL4cg.PU results in a decrease in starvation-induced autophagy in the larval fat body, as compared to controls.
Atg5JF02703, Scer\GAL4NP5130 has decreased cell number | adult stage phenotype, non-suppressible by UvragHMS01357, Scer\GAL4NP5130
Atg5JF02703, Scer\GAL4NP5130 has decreased occurrence of cell division | adult stage phenotype, non-suppressible by UvragHMS01357, Scer\GAL4NP5130
Atg5JF02703, Scer\GAL4bbg-C96 is an enhancer of visible phenotype of Scer\GAL4bbg-C96, domdsRNA.Sym.UAS
Atg5JF02703, Scer\GAL4NP5130 is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4NP5130, UvragHMS01357
Atg5JF02703, Scer\GAL4NP5130 is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4NP5130, UvragHMS01357
Atg5JF02703, Scer\GAL4Cg.PA is a non-suppressor of decreased cell size | third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030
Atg5JF02703, Scer\GAL4NP5130 has adult midgut epithelium phenotype, non-suppressible by UvragHMS01357, Scer\GAL4NP5130
Atg5JF02703, Scer\GAL4NP5130 has intestinal stem cell of posterior adult midgut epithelium phenotype, non-suppressible by UvragHMS01357, Scer\GAL4NP5130
Atg5JF02703, Scer\GAL4bbg-C96 is an enhancer of wing margin phenotype of Scer\GAL4bbg-C96, domdsRNA.Sym.UAS
Atg5JF02703, Scer\GAL4Cg.PA is a suppressor | partially of fat cell | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030
Atg5JF02703, Scer\GAL4Cg.PA is a suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030
Atg5JF02703, Scer\GAL4NP5130 is a suppressor of adult midgut epithelium phenotype of Scer\GAL4NP5130, UvragHMS01357
Atg5JF02703, Scer\GAL4NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4NP5130, UvragHMS01357
Pink1UAS.cCa/Atg5JF02703, Scer\GAL4Cg.PA is a non-suppressor of fat cell | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030
Pink1UAS.cCa/Atg5JF02703, Scer\GAL4Cg.PA is a non-suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4Cg.PA, Tom40JF02030
The adulthood-only co-expression of UvragHMS01357 and Atg5JF02703 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression) leads to the same severely decreased numbers of intestinal stem cells and mitotic figures in the adult midgut epithelium as upon the single expression of Atg5JF02703, rather than the converse increases induced by the single expression of UvragHMS01357.
The wing nicking phenotype caused by expression of domdsRNA.Sym.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also co-expressing Atg5JF02703.