FlyBase Allele Report: Dmel\E2f1[JF02718]

General Information

Symbol

Dmel\E2f1^JF02718

Species

D. melanogaster

Name

FlyBase ID

FBal0242303

Feature type

allele

Associated gene

Dmel\E2f1

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02718}

Also Known As

UAS-E2F1-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02718}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02718

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of prostate cancer

CEA

(Sekar et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates glioblastoma

modeled by Pi3K92E^CAAX.UAS, btl::Egfr^λ.UAS

(Chi et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing E2f1^JF02718 under the control of Scer\GAL4^Cg.PA leads to adipocytes exhibiting reduced perinuclear microtubule concentration and decreased ploidy, without preventing differentiation, as they are still full of lipid droplets. Decreased ploidy is also observed upon conditional expression from L2 onwards (temporal control of expression using Gal80[ts]).

(Sun et al., 2019)

Adult flies expressing E2f1^JF02718 under the control of Scer\GAL4^Dot.PK display nephrocyte functional defects (measured by uptake assays) but their adult lifespan is comparable to controls.

(Fu et al., 2017)

Expression of E2f1^JF02718 in muscle under the control of Scer\GAL4^Mef2.PU results in significant hyperglycaemia.

(Ugrankar et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

E2f1^JF02718, Scer\GAL4^esg.PU is a suppressor of increased cell growth rate | male | adult stage phenotype of Dp^UAS.cDa, E2f1^UAS.cNa, Scer\GAL4^esg.PU

(Sekar et al., 2023)

Phenotype Manifest In

Suppressor of

Statement

Reference

E2f1^JF02718, Scer\GAL4^Cg.PA is a suppressor of fat cell | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^JF02971

(Sun et al., 2019)

E2f1^JF02718, Scer\GAL4^Cg.PA is a suppressor of nucleus | third instar larval stage phenotype of Scer\GAL4^Cg.PA, shot^JF02971

(Sun et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27564

y¹ v¹; P{TRiP.JF02718}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E2F1^RNAi

(Flegel et al., 2016)

E2f1^JF02718

E2f^JF02718

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

Open Close