FlyBase Allele Report: Dmel\SH3PX1[JF02730]

General Information

Symbol

Dmel\SH3PX1^JF02730

Species

D. melanogaster

Name

FlyBase ID

FBal0242314

Feature type

allele

Associated gene

Dmel\SH3PX1

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02730}

Also Known As

UAS-SH3PX1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02730}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02730

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of colorectal cancer

CEA

(Zhang et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of colorectal cancer

is ameliorated by bsk^GD10555

(Zhang et al., 2019)

is ameliorated by dome^HMS01293

(Zhang et al., 2019)

is ameliorated by pan^ΔN.UAS

(Zhang et al., 2019)

is ameliorated by rho^JF03106

(Zhang et al., 2019)

is ameliorated by stg^NIG.1395R

(Zhang et al., 2019)

is ameliorated by Dsor1^KK102276

(Zhang et al., 2019)

is ameliorated by Ets21C^KK103211

(Zhang et al., 2019)

is ameliorated by InR^K1409A.UAS

(Zhang et al., 2019)

is ameliorated by Krn^NIG.8056R

(Zhang et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^P2.4.Pdf

(Liu et al., 2024)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

(Zhang et al., 2019)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU, Scer\GAL80^Su(H).GBE

(Zhang et al., 2019)

increased occurrence of cell division | adult stage, with Scer\GAL4^{Su(H).GBE.cUa}

(Zhang et al., 2019)

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^esg.PU

(Zhang et al., 2019)

adult midgut epithelium, with Scer\GAL4^esg.PU, Scer\GAL80^Su(H).GBE

(Zhang et al., 2019)

adult midgut epithelium, with Scer\GAL4^{Su(H).GBE.cUa}

(Zhang et al., 2019)

larval s-LNv neuron, with Scer\GAL4^P2.4.Pdf

(Liu et al., 2024)

neuron projection | larval stage, with Scer\GAL4^P2.4.Pdf

(Liu et al., 2024)

Detailed Description

Statement

Reference

Adulthood-only expression of SH3PX1^JF02730 under the control of Scer\GAL4^{Su(H).GBE.cUa} or Scer\GAL4^esg.PU alone, or Scer\GAL4^esg.PU and Scer\GAL80^Su(H).GBE combined leads to a severe increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls; this outcome is not obtained when expression is driven by Scer\GAL4^NP0001 or Scer\GAL4^pros-V1. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 7 days during adulthood)

(Zhang et al., 2019)

Larval fat body cells expressing SH3PX1^JF02730 under the control of Scer\GAL4^Act.PU show reduced production of autophagosomes compared to wild-type cells under starvation conditions.

Larval fat body cells expressing SH3PX1^JF02730 under the control of Scer\GAL4^Act.PU also show decreased formation of autophagic compartments in well fed 110 hour old larvae (normally undergoing developmental autophagy).

(Knævelsrud et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

SH3PX1^JF02730, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible by Ets21C^KK103211, Scer\GAL4^esg.PU

(Zhang et al., 2019)

SH3PX1^JF02730, Scer\GAL4^esg.PU has increased occurrence of cell division | adult stage phenotype, suppressible | partially by stg^NIG.1395R, Scer\GAL4^esg.PU