FlyBase Allele Report: Dmel\Nmnat[JF03337]

General Information

Symbol

Dmel\Nmnat^JF03337

Species

D. melanogaster

Name

FlyBase ID

FBal0242510

Feature type

allele

Associated gene

Dmel\Nmnat

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03337}

Also Known As

Nmnat RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03337}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03337

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Fang et al., 2012)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{M337V.UAS.Tag:MYC}

(Huber et al., 2023)

model of neurodegenerative disease

is ameliorated by Mmus\Nmnat1^UAS.cAa

(Fang et al., 2012)

is ameliorated by Mmus\Nmnat2^UAS.cAa

(Fang et al., 2012)

is ameliorated by Mmus\Nmnat3^UAS.cAa

(Fang et al., 2012)

is ameliorated by Mmus\Wld^S.UAS

(Fang et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Rallis et al., 2013)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107, Scer\GAL80^ts.αTub84B

(Rallis et al., 2013)

abnormal neuroanatomy | adult stage, with Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

Phenotype Manifest In

adult mechanosensory neuron, with Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

alpha/beta Kenyon cell | adult stage, with Scer\GAL4^ey-OK107

(Rallis et al., 2013)

alpha/beta Kenyon cell | adult stage, with Scer\GAL4^ey-OK107, Scer\GAL80^ts.αTub84B

(Rallis et al., 2013)

axon | adult stage, with Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

Detailed Description

Statement

Reference

Expression of Nmnat^JF03337 in the fat body under the control of Scer\GAL4^DcG results in significant hyperglycaemia.

Expression of Nmnat^JF03337 in the fat body under the control of Scer\GAL4^DcG has no effect on trehalose levels compared with controls.

(Ugrankar et al., 2015)

28 day old flies expressing Nmnat^JF03337 under the control of Scer\GAL4^ey-OK107 exhibit β-axon loss in the mushroom body. Some neuronal loss is visible in newly-eclosed adults and almost all neurons are lost in 7-day old adults. Expressing Nmnat^JF03337 throughout development produces the most severe phenotype: restricting expression so that it starts at either the pupal or 1d old adult stages using Scer\GAL80^ts.αTub84B results in a less severe phenotype.

(Rallis et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nmnat^JF03337, Scer\GAL4^455.2 has abnormal neuroanatomy | adult stage phenotype, suppressible by Wnk^UAS.cIa, Scer\GAL4^455.2

(Izadifar et al., 2021)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has abnormal neuroanatomy | adult stage phenotype, suppressible by Mmus\Nmnat1^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has abnormal neuroanatomy | adult stage phenotype, suppressible by Mmus\Nmnat3^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has abnormal neuroanatomy | adult stage phenotype, suppressible by Mmus\Nmnat1^dead.UAS, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has abnormal neuroanatomy | adult stage phenotype, suppressible by Mmus\Wld^{S-dead.UAS.Tag:MYC}, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Hsp26^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Hsap\HSPA1L^UAS.cWa, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nmnat^JF03337, Scer\GAL4^455.2 has axon | adult stage phenotype, suppressible by Wnk^UAS.cIa, Scer\GAL4^455.2

(Izadifar et al., 2021)

Nmnat^JF03337, Scer\GAL4^455.2 has adult mechanosensory neuron phenotype, suppressible by Wnk^UAS.cIa, Scer\GAL4^455.2

(Izadifar et al., 2021)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has alpha/beta Kenyon cell | adult stage phenotype, suppressible by Mmus\Nmnat1^dead.UAS, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has alpha/beta Kenyon cell | adult stage phenotype, suppressible by Mmus\Wld^{S-dead.UAS.Tag:MYC}, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has alpha/beta Kenyon cell | adult stage phenotype, suppressible | partially by Hsp26^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has alpha/beta Kenyon cell | adult stage phenotype, suppressible | partially by Hsap\HSPA1L^UAS.cWa, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has alpha/beta Kenyon cell | adult stage phenotype, suppressible by Mmus\Nmnat1^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Nmnat^JF03337, Scer\GAL4^ey-OK107 has alpha/beta Kenyon cell | adult stage phenotype, suppressible by Mmus\Nmnat3^UAS.Tag:MYC, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced axonal degeneration seen in SkpA^GD65 larvae compared to controls after crush injury is suppressed by expression of Nmnat^JF03337 under the control of Scer\GAL4^tey-5053A.

(Brace et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Nmnat1^{dead.Scer\UAS} suppresses the mushroom body degeneration seen in 7 day old flies when Nmnat^JF03337 is expressed under the control of Scer\GAL4^ey-OK107.

Expression of Mmus\wld^{S-dead.Scer\UAS.T:Hsap\MYC} suppresses the mushroom body degeneration seen in 7 day old flies when Nmnat^JF03337 is expressed under the control of Scer\GAL4^ey-OK107.

Expression of Hsp26^{Scer\UAS.T:Ivir\HA1} partially suppresses the mushroom body degeneration seen in 7 day old flies when Nmnat^JF03337 is expressed under the control of Scer\GAL4^ey-OK107.

Expression of Hsap\HSPA1L^Scer\UAS.cWa partially suppresses the mushroom body degeneration seen in 7 day old flies when Nmnat^JF03337 is expressed under the control of Scer\GAL4^ey-OK107.

Expression of Mmus\Nmnat1^{Scer\UAS.T:Hsap\MYC} suppresses the mushroom body degeneration seen in 7 day old flies when Nmnat^JF03337 is expressed under the control of Scer\GAL4^ey-OK107.

Expression of Mmus\Nmnat3^{Scer\UAS.T:Hsap\MYC} suppresses the mushroom body degeneration seen in 7 day old flies when Nmnat^JF03337 is expressed under the control of Scer\GAL4^ey-OK107.

(Rallis et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29402

y¹ v¹; P{TRiP.JF03337}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nmnat^JF03337

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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