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General Information
Symbol
Dmel\Blos1ex2
Species
D. melanogaster
Name
FlyBase ID
FBal0242577
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a deletion which removes sequences from -56 to +38 relative to the ATG start site.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
is exacerbated by Rab321
is exacerbated by cm1
is exacerbated by g2
is ameliorated by Rab11d01994
is exacerbated by rb1
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Blos1ex2 homozygous wandering third instar larvae display a significantly increased rate of depletion and a significantly decreased rate of recovery of the synaptic vesicle pool upon high-frequency stimulation of the neuromuscular junction, as compared to controls.

Both homozygous and heterozygous Blos1ex2 mutant flies have increased miniature excitatory junction potential amplitude and a correspondingly lower quantal content.

Blos1ex2 mutant synaptic vesicles are similar in size to controls.

Homozygous Blos1ex2 mutant flies exhibit deficits in short term olfactory habituation. This phenotype is not observed in either heterozygote.

blos1ex2 mutants exhibit normal homeostatic compensation following application of PhTx, indicating that blos1 is not involved in homeostatic plasticity.

Mutant flies have reduced red pigment in the eye compared to controls.

Homozygous flies show a reduction in both brown and red pigment levels in the retina, in the absence of any gross anatomical abnormality. There are no defects in the organisation of the ommatidia or in the number of photoreceptors. The number and electron density of pigment granules within mutant pigment cells is reduced, and the remaining pigment granules have an abnormal morphology.

The average amplitude of spontaneous miniature excitatory junction potentials (mEJPs) at the neuromuscular junction (NMJ) is approximately 24% larger than that of controls in mutant third instar larvae. The mutant NMJ shows a difference in muscle resting potential compared to wild type, but this difference is too small to account for the difference in mEJP amplitude. The mutant NMJs show no significant difference in the average muscle input resistance, the frequency of mEJP events, the average amplitude of evoked EJPs or the estimated quantal content (EJP/mEJP amplitude ration) compared to controls.

The number of mutant males engaged in male-to-male courtship behaviour during a 10 minute period is significantly higher than that of wild-type flies.

Homozygous flies show locomotor hyperactivity, including rapid jumping and running as well as interrupted flight. The number of physical contacts between flies that occur during a 10 minute period is increased in homozygous flies compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Enhancer of
Statement
Reference

Blos1ex2 is an enhancer of eye color defective phenotype of rb1

Blos1ex2 is an enhancer of eye color defective phenotype of cm1

Blos1ex2 is an enhancer of eye color defective phenotype of g2

Blos1ex2 is an enhancer of eye color defective phenotype of Rab321

NOT Enhancer of
Statement
Reference

Blos1ex2 is a non-enhancer of eye color defective phenotype of ca1

Blos1ex2 is a non-enhancer of eye color defective phenotype of pp

NOT Suppressor of
Statement
Reference

Blos1ex2 is a non-suppressor of eye color defective phenotype of ca1

Blos1ex2 is a non-suppressor of eye color defective phenotype of pp

Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Dysbe01028 Blos1ex2 double heterozygotes exhibit deficits in short term olfactory habituation. This phenotype is not observed in either heterozygote alone.

Expression of auxScer\UAS.cHa under the control of Scer\GAL4GMR.PF enhances the loss of red eye pigment that is seen in blos1ex2 flies.

The increased male-to-male courtship behaviour of blos1ex2 males is suppressed by wunspecified.

The reduction in red pigment in the eye seen in rb1 blos1ex2, cm1 blos1ex2, ltd1 blos1ex2 and g2 blos1ex2 double mutants is more severe than that seen in either single mutant.

The reduced red eye pigment phenotype of ca1 blos1ex2 double mutants is indistinguishable from that of ca1 single mutants.

The reduced red eye pigment phenotype of pp blos1ex2 double mutants is indistinguishable from that of pp single mutants.

Expression of one of P{EPgy2}EY16920, CG9139EP681a, PBac{WH}CG5342f05247, P{EPgy2}EY00704, P{EPgy2}CG5903EY11938 or CG17184EY11874 under the control of Scer\GAL4GMR.PF has no effect on the reduced red eye pigment phenotype of blos1ex2 flies.

Expression of Rab11d01994 under the control of Scer\GAL4GMR.PF partially suppresses the reduced red eye pigment phenotype of blos1ex2 flies.

The loss of red eye pigment that is seen in blos1ex2 flies is enhanced by expression of one of auxFL.Scer\UAS.T:Disc\RFP-mRFP or auxCJ.Scer\UAS.T:Disc\RFP-mRFP under the control of Scer\GAL4GMR.PF.

Expression of auxΔC.Scer\UAS.T:Disc\RFP-mRFP or auxCJ.DLL.DPF.Scer\UAS.T:Disc\RFP-mRFP under the control of Scer\GAL4GMR.PF does not enhance the loss of red eye pigment that is seen in blos1ex2 flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)