Blos1ex2 homozygous wandering third instar larvae display a significantly increased rate of depletion and a significantly decreased rate of recovery of the synaptic vesicle pool upon high-frequency stimulation of the neuromuscular junction, as compared to controls.
Both homozygous and heterozygous Blos1ex2 mutant flies have increased miniature excitatory junction potential amplitude and a correspondingly lower quantal content.
Blos1ex2 mutant synaptic vesicles are similar in size to controls.
Homozygous Blos1ex2 mutant flies exhibit deficits in short term olfactory habituation. This phenotype is not observed in either heterozygote.
blos1ex2 mutants exhibit normal homeostatic compensation following application of PhTx, indicating that blos1 is not involved in homeostatic plasticity.
Mutant flies have reduced red pigment in the eye compared to controls.
Homozygous flies show a reduction in both brown and red pigment levels in the retina, in the absence of any gross anatomical abnormality. There are no defects in the organisation of the ommatidia or in the number of photoreceptors. The number and electron density of pigment granules within mutant pigment cells is reduced, and the remaining pigment granules have an abnormal morphology.
The average amplitude of spontaneous miniature excitatory junction potentials (mEJPs) at the neuromuscular junction (NMJ) is approximately 24% larger than that of controls in mutant third instar larvae. The mutant NMJ shows a difference in muscle resting potential compared to wild type, but this difference is too small to account for the difference in mEJP amplitude. The mutant NMJs show no significant difference in the average muscle input resistance, the frequency of mEJP events, the average amplitude of evoked EJPs or the estimated quantal content (EJP/mEJP amplitude ration) compared to controls.
The number of mutant males engaged in male-to-male courtship behaviour during a 10 minute period is significantly higher than that of wild-type flies.
Homozygous flies show locomotor hyperactivity, including rapid jumping and running as well as interrupted flight. The number of physical contacts between flies that occur during a 10 minute period is increased in homozygous flies compared to controls.