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General Information
Symbol
Dmel\Blos1ex65
Species
D. melanogaster
Name
FlyBase ID
FBal0242578
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a deletion which removes sequences from -56 to +1033 relative to the ATG start site.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Blos1ex65 heterozygous mutant third instar larvae display increased number of boutons on neuromuscular junctions and reduced number of dendritic branches as well as the total dendrite length in class IV dendritic arborizing neurons.

Both homozygous and heterozygous Blos1ex65 mutant flies have increased miniature excitatory junction potential amplitude and a correspondingly lower quantal content.

Homozygous Blos1ex65 mutant flies exhibit deficits in short term olfactory habituation. This phenotype is not observed in either heterozygote.

Blos1ex65 mutants show a dominant increase in the number of boutons per muscle at the 6/7 synapse, but overall muscle area is unaffected.

Homozygous flies show a reduction in both brown and red pigment levels in the retina, in the absence of any gross anatomical abnormality. There are no defects in the organisation of the ommatidia or in the number of photoreceptors. The number and electron density of pigment granules within mutant pigment cells is reduced, and the remaining pigment granules have an abnormal morphology.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Blos1ex65 has neuroanatomy defective | dominant phenotype, non-suppressible by Dysbrv/Dysb[+]

Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

Blos1ex65 has NMJ bouton | supernumerary phenotype, non-suppressible by Dysbrv/Dysb[+]

Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The increased number of boutons on neuromuscular junctions observed in third instar larvae heterozygous for any of the following alleles: Arpc1Q25sd, Arpc1Q25st or Blos1ex65 is restored to wild-type levels in both Arpc1Q25sd/+;Blos1ex65/+ and Arpc1Q25st/+;Blos1ex65/+ double heterozygotes.

The reduced number of dendritic branches in class IV dendritic arborizing neurons characteristic for Blos1ex65 heterozygous larvae is mildly enhanced in Blos1ex65;Arpc1Q25sd double heterozygotes but the total dendrite length per neuron remains unchanged.

One copy of Dysbe01028 suppresses the dominant changes in mEJP amplitude and quantal content seen in Blos1ex65/+ mutant NMJs. The increase in bouton number seen in Blos1ex65/+ NMJs is also suppressed. Dysbe01028 Blos1ex65 double heterozygotes exhibit deficits in short term olfactory habituation. This phenotype is not observed in either heterozygote alone.

One copy of Dysbrv does not suppress the increase in bouton number seen in Blos1ex65/+ NMJs.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Pre-synaptic expression of Blos1Scer\UAS.cCa under the control of Scer\GAL4elav-C155 rescues the changes in mEJP amplitude seen in Blos1ex65 mutant NMJs.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)