FlyBase Allele Report: Dmel\VGlut1[UAS.EGFP]

General Information

Symbol

Dmel\VGlut1^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0242588

Feature type

allele

Associated gene

Dmel\VGlut1

Associated Insertion(s)

Carried in Construct

P{UAS-VGlut1.GFP}

Also Known As

UAS-GFP:DVGLUT

Key Links

Transgenic product class

wild_type

(Daniels et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Daniels et al., 2008)

Mutagen

in vitro construct

(Daniels et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-VGlut1.GFP}

Cytology

Description

UASt regulatory sequences drive expression of VGlut, which is tagged at the N-terminal end with EGFP.

(Daniels et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

VGlut1

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^VGlut1.PD

(Daniels et al., 2011)

lethal, with Scer\GAL4^VGlut1.PD

(Daniels et al., 2011)

lethal - all die before end of pupal stage, with Scer\GAL4^VGlut1.PD

(Grygoruk et al., 2010)

some die during pupal stage, with Scer\GAL4^VGlut1.PD

(Grygoruk et al., 2010)

Phenotype Manifest In

antennal lobe, with Scer\GAL4^VGlut1.PD

(Daniels et al., 2011)

neuropil, with Scer\GAL4^VGlut1.PD

(Daniels et al., 2011)

Detailed Description

Statement

Reference

Flies overexpressing VGlut^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^VGlut.PD show progressive degeneration throughout most regions of the neuropil.

When VGlut^{Scer\UAS.T:Avic\GFP-EGFP} is driven in glutamatergic neurons by Scer\GAL4^VGlut.PD, there is degeneration in the antennal lobe.

When VGlut^{Scer\UAS.T:Avic\GFP-EGFP} is driven in cholinergic olfactory receptor neurons (ORNs) by Scer\GAL4^Or83b.PK, no degeneration is apparent.

The majority of flies expressing VGlut^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^VGlut.PD die before 2 weeks of age.

(Daniels et al., 2011)

Animals expressing VGlut^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^VGlut.PD generally die as pupae; rare escapers eclose but their wings fail to inflate.

(Grygoruk et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^VGlut1.PD, Su(VGlut)B6^B6, VGlut1^UAS.EGFP has viable phenotype

(Grygoruk et al., 2010)

Scer\GAL4^VGlut1.PD, Su(VGlut)B8^B8, VGlut1^UAS.EGFP has viable phenotype