FlyBase Allele Report: Dmel\erm[UAS.Tag:HA]

General Information

Symbol

Dmel\erm^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0242762

Feature type

allele

Associated gene

Dmel\erm

Associated Insertion(s)

Carried in Construct

P{UAS-erm.HA}

Also Known As

UAS-erm

Key Links

Transgenic product class

cDNA

(Weng et al., 2010)

wild_type

(Weng et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Weng et al., 2010)

wild_type

(Weng et al., 2010)

Mutagen

in vitro construct

(Weng et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-erm.HA}

Cytology

Description

UASt regulatory sequences drive expression of an erm cDNA tagged with a Tag:HA epitope.

(Weng et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

erm

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^wor.PA

(Janssens et al., 2014, Weng et al., 2010)

Phenotype Manifest In

larval neuroblast, with Scer\GAL4^wor.PA

(Weng et al., 2010)

type II neuroblast | third instar larval stage, with Scer\GAL4^wor.PA

(Janssens et al., 2014)

Detailed Description

Statement

Reference

Expression of erm^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR9D11 does not result in a significant change in the number of Asense negative type II neuroblasts, as compared to wild type.

(Xie et al., 2016)

Expression of erm^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^wor.PA induces premature differentiation of all eight type II neuroblasts in a larval brain lobe.

(Janssens et al., 2014)

Expression of erm^{Scer\UAS.T:Ivir\HA} induced by the neuroblast-specific Scer\GAL4^wor.PA leads to premature loss of type II neuroblasts in the larval brain.

Expression of erm^{Scer\UAS.T:Ivir\HA} induced by Scer\GAL4^{CG31670.R9D11} fails to exert any effect on type II neuroblasts in the larval brain.

(Weng et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^wor.PU, Scer\GAL80^ase.PN, erm^UAS.Tag:HA is a suppressor of abnormal cell number | somatic clone | larval stage phenotype of IntS8^SH1314

(Zhang et al., 2019)

Scer\GAL4^wor.PU, Scer\GAL80^ase.PN, erm^UAS.Tag:HA is a suppressor of abnormal neuroanatomy | somatic clone | larval stage phenotype of IntS8^SH1314

(Zhang et al., 2019)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a suppressor of increased cell number | larval stage phenotype of pnt^P1-90

(Xie et al., 2016)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a suppressor of abnormal neuroanatomy | larval stage phenotype of pnt^P1-90

(Xie et al., 2016)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^GMR9D11, dpn^UAS.cWa

(Janssens et al., 2014)

erm^UAS.Tag:HA, Scer\GAL4^GMR9D11 is a suppressor of abnormal neuroanatomy phenotype of E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a suppressor of abnormal neuroanatomy | somatic clone phenotype of brat^DG19310/brat¹¹, erm[+]/erm¹

(Janssens et al., 2014)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a suppressor of abnormal neuroanatomy phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

erm^UAS.Tag:HA, Scer\GAL4^GMR9D11 is a suppressor of abnormal neuroanatomy phenotype of N^intra.GS.UAS, Scer\GAL4^GMR9D11

(Weng et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a non-suppressor of abnormal neuroanatomy phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

Scer\GAL4^elav-C155/erm^UAS.Tag:HA is a non-suppressor of abnormal neuroanatomy | somatic clone phenotype of pros¹⁷

(Weng et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^wor.PU, Scer\GAL80^ase.PN, erm^UAS.Tag:HA is a suppressor of type II neuroblast | increased number | somatic clone | larval stage phenotype of IntS8^SH1314

(Zhang et al., 2019)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a suppressor of type II neuroblast | larval stage phenotype of pnt^P1-90

(Xie et al., 2016)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a suppressor of type II neuroblast phenotype of Scer\GAL4^GMR9D11, dpn^UAS.cWa

(Janssens et al., 2014)

erm^UAS.Tag:HA, Scer\GAL4^GMR9D11 is a suppressor of type II neuroblast phenotype of E(spl)mγ-HLH^UAS.cLa, Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a suppressor of type II neuroblast | somatic clone phenotype of brat^DG19310/brat¹¹, erm[+]/erm¹

(Janssens et al., 2014)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a suppressor of type II neuroblast | increased number phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

erm^UAS.Tag:HA, Scer\GAL4^GMR9D11 is a suppressor of larval neuroblast | ectopic phenotype of N^intra.GS.UAS, Scer\GAL4^GMR9D11

(Weng et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA is a non-suppressor of type II neuroblast | increased number phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

Scer\GAL4^elav-C155/erm^UAS.Tag:HA is a non-suppressor of larval neuroblast | ectopic | somatic clone phenotype of pros¹⁷

(Weng et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of erm^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR9D11 significantly suppresses the increase in type II neuroblasts seen in pnt^P1-90/pnt^P1-90 mutant larvae.

(Xie et al., 2016)

In contrast to brat^DG19310/brat¹¹ clones alone, clones originating from either a single ase+ immature Intermediate Neural Progenitor (INP) or INP that are also heterozygous for erm¹ contain an increased number of neuroblasts 'reverted clones'. This phenotype is reversed upon expression of erm^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR9D11. This rescue is seen using an insertion line of the driver that expressed in ase[-] INPs (P{GMR9D11-GAL4}2) and one that expresses in ase[+] INPs (P{GMR9D11-GAL4}attP2).

Expression of erm^{Scer\UAS.T:Ivir\HA1} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2) suppresses the supernumerary neuroblast phenotype seen in brat^DG19310/brat¹¹ mutant larval brains. Expression of erm^{Scer\UAS.T:Ivir\HA1} using a line that expresses in ase[+] INPs (P{GMR9D11-GAL4}attP2) does not suppress the phenotype.

Expression of erm^{Scer\UAS.T:Ivir\HA1} suppresses the supernumerary neuroblast phenotype seen in larval brains when dpn^Scer\UAS.cWa is expressed under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2).

Expression of erm^{Scer\UAS.T:Ivir\HA1} suppresses the supernumerary type II neuroblasts phenotype seen in larval brains when klu^{Scer\UAS.T:Ivir\HA1} is expressed under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2).

Expression of erm^{Scer\UAS.T:Ivir\HA1} suppresses the supernumerary type II neuroblasts phenotype seen in larval brains when E(spl)mγ-HLH^Scer\UAS.cLa is expressed under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2).

(Janssens et al., 2014)

Overexpression of erm^{Scer\UAS.T:Ivir\HA} under the control of Scer\GAL4^elav-C155 fails to suppress ectopic Intermediate Neural Progenitors in pros¹⁷ mutant type II neuroblast clones in the larval brain.

Coexpression of erm^{Scer\UAS.T:Ivir\HA} under the control of Scer\GAL4^{CG31670.R9D11} suppresses the ectopic neuroblasts induced by expression of N^{intra.GS.Scer\UAS}.

(Weng et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^9-9/erm^UAS.Tag:HA rescues erm¹

(Santiago et al., 2021)

Scer\GAL4^GMR9D11/erm^UAS.Tag:HA rescues Df(2L)erm²/erm¹

(Janssens et al., 2014)

Comments

Expression of erm^{Scer\UAS.T:Ivir\HA1} under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) suppresses the supernumerary neuroblast phenotype seen in erm¹/Df(2L)erm² mutant larval brains.

(Janssens et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

erm^{Scer\UAS.T:Ivir\HA1}

erm^{Scer\UAS.T:Ivir\HA}

erm^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (10)

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