Allele Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}

General Information
Symbol	Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}	Species	H. sapiens
Name		FlyBase ID	FBal0242934
Feature type	allele	Associated gene	Hsap\ACSL4

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Zhang et al., 2009) in vitro construct - regulatory fusion (Zhang et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Carried in construct	P{UAS-ACSL4.L.Myc} (Zhang et al., 2009, Liu et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^tub.PU is a suppressor \| partially of lethal phenotype of Acsl¹/Acsl⁸ (Zhang et al., 2009) Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^tub.PU is a suppressor \| partially of lethal phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸ (Zhang et al., 2009) Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^tub.PU is a suppressor of neuroanatomy defective \| third instar larval stage phenotype of Acsl⁸/Acsl^k10313 (Zhang et al., 2009) Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^αTub84B.PL is a suppressor of neuroanatomy defective \| larval stage phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO (Liu et al., 2011) Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^αTub84B.PL is a suppressor of neurophysiology defective phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO (Liu et al., 2011) Scer\GAL4^elav.PU/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} is a suppressor of neurophysiology defective phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO (Liu et al., 2011) Scer\GAL4^Mhc.PW/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} is a suppressor \| partially of neurophysiology defective phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO (Liu et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^tub.PU is a suppressor of embryonic/larval brain \| third instar larval stage phenotype of Acsl⁸/Acsl^k10313 (Zhang et al., 2009) Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^tub.PU is a suppressor of phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸ (Zhang et al., 2009) Scer\GAL4^elav.PU/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} is a suppressor of bouton phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO (Liu et al., 2011) Scer\GAL4^{elav.Switch.PO}/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} is a suppressor of synapse phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO (Liu et al., 2011)
NOT Suppressor of
Statement Reference Scer\GAL4^Mhc.PW/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} is a non-suppressor of bouton phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO (Liu et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] in postsynaptic muscles driven by Scer\GAL4[Mhc.PW] does not suppress the dystrophic neuromuscular junctions of Acsl[KO]/Acsl[05847] mutants for bouton number. Neuronal expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] at 2.5 days after egg laying (AEL) fully suppresses the reduced synaptic area phenotype of Acsl[KO]/Acsl[05847] mutants. Neuronal expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] driven by Scer\GAL4[elav.PU] restores the bouton number to wild-type levels in Acsl[KO]/Acsl[05847] mutants. The neuromuscular junction phenotypes of Acsl[KO]/Acsl[05847] mutants can be fully suppressed by ubiquitous expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[αTub84B.PL]. (Liu et al., 2011) Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[tub] partially rescues the lethality associated with the Acsl[8]/Acsl[1] and Acsl[8]/Acsl[05847] genotypes. Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[tub] restores neutral lipid staining in Acsl[8]/Acsl[05847] wing discs. Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[tub] restores normal triacylglycerol levels in Acsl[8]/Acsl[05847] wing discs. Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[tub] restores well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl[8]/Acsl[k10313] mutants. (Zhang et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Scer\UAS sequences drive expression of the long form of Hsap\ACSL4 with a C-terminal 6x T:Hsap\MYC tag. (Zhang et al., 2009)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Liu et al., 2011, J. Neurosci. 31(6): 2052--2063 Drosophila Acyl-CoA Synthetase Long-Chain Family Member 4 Regulates Axonal Transport of Synaptic Vesicles and Is Required for Synaptic Development and Transmission. [FBrf0212968] Zhang et al., 2009, Hum. Mol. Genet. 18(20): 3894--3905 Analyses of mental dysfunction-related ACSl4 in Drosophila reveal its requirement for Dpp/BMP production and visual wiring in the brain. [FBrf0209077]

Allele Hsap\ACSL4Scer\UAS.L.T:Hsap\MYC

Allele Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}