FlyBase Allele Report: Hsap\ACSL4[UAS.L.Tag:MYC]

General Information

Symbol

Hsap\ACSL4^{UAS.L.Tag:MYC}

Species

H. sapiens

Name

FlyBase ID

FBal0242934

Feature type

allele

Associated gene

Hsap\ACSL4

Associated Insertion(s)

Carried in Construct

P{UAS-ACSL4.L.Myc}

Also Known As

UAS-ACSL4-Myc, UAS-ACSL4

Key Links

Transgenic product class

wild_type

(Zhang et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Zhang et al., 2009)

Mutagen

in vitro construct

(Zhang et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-ACSL4.L.Myc}

Cytology

Description

UAS sequences drive expression of the long form of Hsap\ACSL4 with a C-terminal 6x Tag:MYC tag.

(Zhang et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\ACSL4

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of non-syndromic X-linked intellectual disability 63

CEA

(Liu et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates non-syndromic X-linked intellectual disability 63

modeled by Acsl⁰⁵⁸⁴⁷, Acsl⁸

(Zhang et al., 2009)

modeled by Acsl⁰⁵⁸⁴⁷, Acsl^KO

(Huang et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC} is a suppressor of abnormal neuroanatomy | larval stage phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^αTub84B.PL is a suppressor of abnormal neuroanatomy | larval stage phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2011)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^αTub84B.PL is a suppressor of abnormal neurophysiology phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2011)

Scer\GAL4^elav.PU/Hsap\ACSL4^{UAS.L.Tag:MYC} is a suppressor of abnormal neurophysiology phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2011)

Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC} is a suppressor | partially of abnormal neurophysiology phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2011)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU is a suppressor | partially of lethal phenotype of Acsl¹/Acsl⁸

(Zhang et al., 2009)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU is a suppressor | partially of lethal phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸

(Zhang et al., 2009)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Acsl⁸/Acsl^k10313

(Zhang et al., 2009)

NOT Suppressor of

Statement

Reference

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC} is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC} is a suppressor of neuromuscular junction phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC} is a suppressor of larval abdominal 2 neuron phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC} is a suppressor of larval abdominal 3 neuron phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Scer\GAL4^elav-C155/Hsap\ACSL4^{UAS.L.Tag:MYC} is a suppressor of NMJ bouton | increased number phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^αTub84B.PL is a suppressor of phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Scer\GAL4^elav.PU/Hsap\ACSL4^{UAS.L.Tag:MYC} is a suppressor of bouton phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2011)

Scer\GAL4^{elav.Switch.PO}/Hsap\ACSL4^{UAS.L.Tag:MYC} is a suppressor of synapse phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2011)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU is a suppressor of larval brain | third instar larval stage phenotype of Acsl⁸/Acsl^k10313

(Zhang et al., 2009)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU is a suppressor of phenotype of Acsl⁰⁵⁸⁴⁷/Acsl⁸

(Zhang et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC} is a non-suppressor of neuromuscular junction phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC} is a non-suppressor of larval abdominal 2 neuron phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC} is a non-suppressor of larval abdominal 3 neuron phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC} is a non-suppressor of NMJ bouton | increased number phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo is a non-suppressor of neuromuscular junction phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo is a non-suppressor of larval abdominal 2 neuron phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo is a non-suppressor of larval abdominal 3 neuron phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^repo is a non-suppressor of NMJ bouton | increased number phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2014)

Scer\GAL4^Mhc.PW/Hsap\ACSL4^{UAS.L.Tag:MYC} is a non-suppressor of bouton phenotype of Acsl⁰⁵⁸⁴⁷/Acsl^KO

(Liu et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^elav-C155-, but not Scer\GAL4^Mhc.PW- or Scer\GAL4^repo-mediated expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} rescues the increased total and satellite bouton number in neuromuscular junction 4 of the anterior segments A2 and A3 in Acsl⁰⁵⁸⁴⁷/Acsl^KO animals.

The increase in phospho-Mad levels in the anterior neuromuscular junctions of Acsl⁰⁵⁸⁴⁷/Acsl^KO mutants is suppressed by Scer\GAL4^αTub84B.PL-mediated expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}.

(Liu et al., 2014)

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} in postsynaptic muscles driven by Scer\GAL4^Mhc.PW does not suppress the dystrophic neuromuscular junctions of Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants for bouton number.

Neuronal expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} at 2.5 days after egg laying (AEL) fully suppresses the reduced synaptic area phenotype of Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants.

Neuronal expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} driven by Scer\GAL4^elav.PU restores the bouton number to wild-type levels in Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants.

The neuromuscular junction phenotypes of Acsl^KO/Acsl⁰⁵⁸⁴⁷ mutants can be fully suppressed by ubiquitous expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^αTub84B.PL.

(Liu et al., 2011)

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub partially rescues the lethality associated with the Acsl⁸/Acsl¹ and Acsl⁸/Acsl⁰⁵⁸⁴⁷ genotypes.

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub restores neutral lipid staining in Acsl⁸/Acsl⁰⁵⁸⁴⁷ wing discs.

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub restores normal triacylglycerol levels in Acsl⁸/Acsl⁰⁵⁸⁴⁷ wing discs.

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub restores well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl⁸/Acsl^k10313 mutants.

(Zhang et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64682

w^*; P{UAS-ACSL4.L.Myc}3

Notes on Origin

Discoverer

Comments

Scer\UAS sequences drive expression of the long form of Hsap\ACSL4 with a C-terminal 6x T:Hsap\MYC tag.

(Zhang et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}

Hsap\ACSL4^{UAS.L.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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