Drives expression in the prothoracic gland innervating neuron PG-LP in larvae and pharate adults.
GFP reconstitution across synaptic partners (GRASP) shows synaptic connections between Scer\GAL4Ptth.cMa-positive prothoracic gland innervating neurons and Ecol\lexAGMR19B09-positive dorsomedial neurosecretory cells.
Inhibiting Scer\GAL4Ptth.cMa-expressing prothoracic gland neurons by expressing Hsap\KCNJ2UAS.EGFP, Ctet\TeTxLCTNT.UAS or grimUAS.cNa induces a developmental delay from L1 to pupariation, as compared to controls; this effect is not observed when using Ctet\TeTxLCIMPTNT-V.UAS.
Conversely, activating Scer\GAL4Ptth.cMa-expressing prothoracic gland neurons by temperature-induced TrpA1UAS.(B).cKa expression induces faster developmental acceleration to pupariation, as compared to controls.
Ablation (using grimUAS.cNa) or silencing (using Hsap\KCNJ2UAS.EGFP, Ork1Δ-C.UAS.EGFP or ShEKO.UAS.GFP(GL)) of Scer\GAL4Ptth.cMa neurons reduces the circadian rhythmicity of adult emergence after transfer to constant darkness, compared to controls; in the case of grimUAS.cNa and Hsap\KCNJ2UAS.EGFP, this is significant; the effects of ablation (using grimUAS.cNa) of Scer\GAL4Ptth.cMa neurons do not occur when flies are maintained on a light-dark or warm-cold circadian cycle.
Hsap\KCNJ2UAS.EGFP, Scer\GAL4Ptth.cMa has abnormal developmental rate | larval stage phenotype, enhanceable by Ptth120F2A
Scer\GAL4Ptth.cMa, TrpA1UAS.(B).cKa has abnormal developmental rate | larval stage phenotype, suppressible by Ptth120F2A
Scer\GAL4Ptth.cMa/TrpA1UAS.(B).cKa is a suppressor of abnormal developmental rate | larval stage phenotype of Ptth120F2A
Ptth120F2A mutants with activated Scer\GAL4Ptth.cMa-expressing prothoracic gland neurons (using TrpA1UAS.(B).cKa) show normal developmental time to pupariation, instead of the delay observed in Ptth120F2A mutants or the acceleration induced by Scer\GAL4Ptth.cMa neurons activation.
The developmental delay from L1 to pupariation induced by the inhibition of Scer\GAL4Ptth.cMa-expressing prothoracic gland neurons (using Hsap\KCNJ2UAS.EGFP) is enhanced by Ptth120F2A.