FlyBase Allele Report: Scer\GAL4[Ptth.cMa]

General Information

Symbol

Scer\GAL4^Ptth.cMa

Species

S. cerevisiae

Name

Ptth promoter construct a of McBrayer

FlyBase ID

FBal0243026

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Ptth-GAL4.M}

Also Known As

PTTH-GAL4

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(McBrayer et al., 2007)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{Ptth-GAL4.M}

Cytology

Nature of the lesion

Statement

Reference

An approximately 1 kb Ptth upstream promoter/enhancer sequence regulates expression of a GAL4 driver.

(McBrayer et al., 2007)

Allele components

Product class / Tool use(s)

Regulatory region(s)

Ptth

Encoded product / tool

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

prothoracic gland innervating neuron PG-LP

(McBrayer et al., 2007)

late third instar larval stage

prothoracic gland innervating neuron PG-LP

(Colombani et al., 2015)

pharate adult stage

adult prothoracic gland innervating neuron PG-LP

(Selcho et al., 2017)

Additional Information

Statement

Reference

GFP reconstitution across synaptic partners (GRASP) shows synaptic connections between Scer\GAL4^Ptth.cMa-positive prothoracic gland innervating neurons and Ecol\lexA^GMR19B09-positive dorsomedial neurosecretory cells.

(Colombani et al., 2015)

Marker for

adult prothoracic gland innervating neuron PG-LP

(Selcho et al., 2017)

Reflects expression of

Ptth

(Selcho et al., 2017, Colombani et al., 2015, McBrayer et al., 2007)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

cell lethal, with grim^UAS.cNa

(McBrayer et al., 2007)

courtship behavior defective | male limited, with grim^UAS.cNa

(McBrayer et al., 2007)

decreased fecundity | female, with grim^UAS.cNa

(McBrayer et al., 2007)

developmental rate defective, with grim^UAS.cNa

(Lin et al., 2013, McBrayer et al., 2007)

developmental rate defective, with InR^E19

(Lin et al., 2013)

developmental rate defective | larval stage, with Ctet\tetX^TNT-LC.UAS

(Shimell et al., 2018)

developmental rate defective | larval stage, with grim^UAS.cNa

(Shimell et al., 2018)

developmental rate defective | larval stage, with Hsap\KCNJ2^UAS.EGFP

(Shimell et al., 2018)

developmental rate defective | larval stage, with TrpA1^UAS.(B).cKa

(Shimell et al., 2018)

eclosion rhythm defective, with Hsap\KCNJ2^UAS.EGFP

(Selcho et al., 2017)

eclosion rhythm defective, with Ork1^{Δ-C.UAS.EGFP}

(Selcho et al., 2017)

eclosion rhythm defective, with Ptth^KK110502

(Selcho et al., 2017)

eclosion rhythm defective, with Sh^{EKO.UAS.GFP(GL)}

(Selcho et al., 2017)

eclosion rhythm defective, with sNPF-R^{dsRNA.UAS.cUa}

(Selcho et al., 2017)

eclosion rhythm defective | conditional, with grim^UAS.cNa

(Selcho et al., 2017)

increased cell number, with grim^UAS.cNa

(McBrayer et al., 2007)

large body, with grim^UAS.cNa

(McBrayer et al., 2007)

neuroanatomy defective, with grim^UAS.cNa

(Lin et al., 2013)

neuroanatomy defective, with InR^E19

(Lin et al., 2013)

neuroanatomy defective, with InR^miRNA.UAS

(Lin et al., 2013)

neuroanatomy defective, with sle⁰⁵⁷

(Lin et al., 2013)

partially lethal - majority die, with grim^UAS.cNa

(McBrayer et al., 2007)

Phenotype Manifest In

alpha/beta Kenyon cell, with grim^UAS.cNa

(Lin et al., 2013)

alpha/beta Kenyon cell, with InR^E19

(Lin et al., 2013)

alpha/beta Kenyon cell, with sle⁰⁵⁷

(Lin et al., 2013)

alpha'/beta' Kenyon cell, with grim^UAS.cNa

(Lin et al., 2013)

alpha'/beta' Kenyon cell, with InR^E19

(Lin et al., 2013)

alpha'/beta' Kenyon cell, with sle⁰⁵⁷

(Lin et al., 2013)

gamma Kenyon cell, with grim^UAS.cNa

(Lin et al., 2013)

gamma Kenyon cell, with InR^E19

(Lin et al., 2013)

gamma Kenyon cell, with sle⁰⁵⁷

(Lin et al., 2013)

mushroom body, with grim^UAS.cNa

(Lin et al., 2013)

mushroom body, with InR^E19

(Lin et al., 2013)

mushroom body, with InR^miRNA.UAS

(Lin et al., 2013)

mushroom body, with sle⁰⁵⁷

(Lin et al., 2013)

prothoracic gland innervating neuron PG-LP, with grim^UAS.cNa

(McBrayer et al., 2007)

wing, with grim^UAS.cNa

(McBrayer et al., 2007)

Detailed Description

Statement

Reference

Inhibiting Scer\GAL4^Ptth.cMa-expressing prothoracic gland neurons by expressing Hsap\KCNJ2^UAS.EGFP, Ctet\TeTxLC^TNT.UAS or grim^UAS.cNa induces a developmental delay from L1 to pupariation, as compared to controls; this effect is not observed when using Ctet\TeTxLC^IMPTNT-V.UAS.

Conversely, activating Scer\GAL4^Ptth.cMa-expressing prothoracic gland neurons by temperature-induced TrpA1^UAS.(B).cKa expression induces faster developmental acceleration to pupariation, as compared to controls.

(Shimell et al., 2018)

Ablation (using grim^UAS.cNa) or silencing (using Hsap\KCNJ2^UAS.EGFP, Ork1^{Δ-C.UAS.EGFP} or Sh^{EKO.UAS.GFP(GL)}) of Scer\GAL4^Ptth.cMa neurons reduces the circadian rhythmicity of adult emergence after transfer to constant darkness, compared to controls; in the case of grim^UAS.cNa and Hsap\KCNJ2^UAS.EGFP, this is significant; the effects of ablation (using grim^UAS.cNa) of Scer\GAL4^Ptth.cMa neurons do not occur when flies are maintained on a light-dark or warm-cold circadian cycle.

(Selcho et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^Ptth.cMa has developmental rate defective | larval stage phenotype, enhanceable by Ptth^120F2A

(Shimell et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^Ptth.cMa, TrpA1^UAS.(B).cKa has developmental rate defective | larval stage phenotype, suppressible by Ptth^120F2A

(Shimell et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^Ptth.cMa/TrpA1^UAS.(B).cKa is a suppressor of developmental rate defective | larval stage phenotype of Ptth^120F2A

(Shimell et al., 2018)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Ptth^120F2A mutants with activated Scer\GAL4^Ptth.cMa-expressing prothoracic gland neurons (using TrpA1^UAS.(B).cKa) show normal developmental time to pupariation, instead of the delay observed in Ptth^120F2A mutants or the acceleration induced by Scer\GAL4^Ptth.cMa neurons activation.

(Shimell et al., 2018)

Xenogenetic Interactions

Statement

Reference

The developmental delay from L1 to pupariation induced by the inhibition of Scer\GAL4^Ptth.cMa-expressing prothoracic gland neurons (using Hsap\KCNJ2^UAS.EGFP) is enhanced by Ptth^120F2A.

(Shimell et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^Ptth.cMa

Name Synonyms

Ptth promoter construct a of McBrayer

Secondary FlyBase IDs

References (16)

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