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General Information
Symbol
Dmel\lncRNA:HsrωdsRNA.Sym.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0243042
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-hsrω-RNAi, UAS-hsrωRNAi, UAS-hsrω RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
Sense-antisense transcription (driven by two convergent arrays of UAS sequence) of the 280 bp repeat motif that is specific to the lncRNA:Hsrω 'n' transcript results in double stranded RNA (dsRNA) expression.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of HsrωdsRNA.Sym.Scer\UAS (either one or two copies, effects of the double RNAi are more severe) under the control of Scer\GAL4elav.PLu results in reduced locomotive capacity in both larvae and adults, decreased adult lifespan as well as reduced total synaptic branch length and bouton number in larval neuromuscular junctions (NMJ). Expression under the Scer\GAL4Toll-6-D42 driver also affects the larval NMJ structure: the total branch length and number along with the number of boutons (total as well as satellite) and the area of terminal boutons are all significantly decreased compared to controls. Expression of HsrωdsRNA.Sym.Scer\UAS under the control of Scer\GAL4GMR.PS does not induce any eye morphology aberrations.
Expression of a single copy of lncRNA:HsrωdsRNA.Sym.UAS under the control of Scer\GAL4dpp.blk1 causes a mild disruption of photoreceptor cells; expression of two copies causes high mortality during development and severe disruption of photoreceptors. Expression of a single copy of lncRNA:HsrωdsRNA.Sym.UAS under the control of Scer\GAL4elav-C155 has no effect on lifespan and does not result in any obvious external phenotype. Expression of a single copy of lncRNA:HsrωdsRNA.Sym.UAS under the control of Scer\GAL4ptc-559.1 does not cause any observable phenotypic effects; expression of two copies causes substantial lethality. Expression of a one or two copies of lncRNA:HsrωdsRNA.Sym.UAS under the control of Scer\GAL4ap-md544 results in ribbon-like wings. Expression of a single copy of lncRNA:HsrωdsRNA.Sym.UAS under the control of Scer\GAL4C805 does not cause any observable phenotypic effects; expression of two copies causes partial lethality during the pupal stage, survivors are very weak but fertile. Expression of lncRNA:HsrωdsRNA.Sym.UAS under the control of Scer\GAL4c825 does not result in sex comb defects. Expression of lncRNA:HsrωdsRNA.Sym.UAS under the control of Scer\GAL4Act5C.PI causes a majority of animals to die before adult stage, with higher mortality in lncRNA:HsrωdsRNA.Sym.UAS homozygotes than heterozygotes; most lethality occurs during the larval stage; surviving adults are fertile, but the gender ratio is skewed in favor of males. Expression of lncRNA:HsrωdsRNA.Sym.UAS under the control of Scer\GAL4Act5C.PI also reduces the number of omega speckles in the nucleus.
Scer\GAL4Act5C.PI-mediated expression of P{Sym-UAS-Hsrω} results in significant embryonic and larval lethality. Expression of one copy of P{Sym-UAS-Hsrω} using Scer\GAL4GMR.PF at 25[o]C results in well organized ommatidial units in larval eye discs.
Scer\GAL4elav-C155-driven expression of P{Sym-UAS-Hsrω} does not have any adverse effect on survival or longevity of flies.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
NOT suppressed by
NOT Enhancer of
Suppressor of
Statement
Reference
lncRNA:HsrωdsRNA.Sym.UAS/ lncRNA:Hsromega [+] is a suppressor of visible | adult stage phenotype of Scer\GAL4GMR.PU
Scer\GAL4ap-md544, lncRNA:HsrωdsRNA.Sym.UAS, lncRNA:Hsromega [+] is a suppressor of lethal | recessive phenotype of apmd544
NOT Suppressor of
Other
Phenotype Manifest In
NOT Enhanced by
NOT suppressed by
NOT Enhancer of
Suppressor of
Statement
Reference
lncRNA:HsrωdsRNA.Sym.UAS/ lncRNA:Hsromega [+] is a suppressor of eye phenotype of Scer\GAL4GMR.PU
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
Third instar larvae simultaneously expressing both HsrωdsRNA.Sym.Scer\UAS and cazVDRC.cUa under the control of Scer\GAL4elav.PLu display crawling ability deficiency as well as morphological defects in the neuromuscular junctions (reduced total synaptic branch length and number of boutons) but the severity of each of these defects is not significantly different from either of the single knockdowns. The eye morphology defects (rough appearance with fused ommatidia) characteristic for flies expressing cazVDRC.cUa under the control of Scer\GAL4GMR.PS can be rescued by co-expression of HsrωdsRNA.Sym.Scer\UAS.
Expression of LamCUAS.cGa and lncRNA:HsrωdsRNA.Sym.UAS under the control of Scer\GAL4Act5C.PI delays the lethality seen with LamCUAS.cGa/Scer\GAL4Act5C.PI, so that most die as pupae or pharate adults; the majority of pharates show a severe head capsule defect, with loss of most head structures; the few adults that emerge are all female.
Expression of HsrωdsRNA.Sym.Scer\UAS under the control of Scer\GAL4ey.PH suppresses the defects seen in homozygous Iswi2 eyes. Expression of HsrωdsRNA.Sym.Scer\UAS under the control of Scer\GAL4ey.PH suppresses the X chromosome condensation defects seen in the polytene chromosomes of Iswi1/Iswi2 male larvae. Co-expression of HsrωdsRNA.Sym.Scer\UAS suppresses the mutant eye and polytene chromosome phenotypes caused by expression of IswiK159R.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ey.PH.
Coexpression of one copy of P{Sym-UAS-Hsrω} in the Scer\GAL4GMR.PF, nejF2161A.Scer\UAS.T:SV5\V5 background substantially improves eye morphology and ommatidial arrays. Co-expression of P{Sym-UAS-Hsrω} in the Scer\GAL4GMR.PF, nejdsRNA.Scer\UAS.cKa background significantly improves pigmentation and eye morphology, including in the posterior part. More embryos/larvae die when nejdsRNA.Scer\UAS.cKa and P{Sym-UAS-Hsrω} are co-expressed with Scer\GAL4Act5C.PI than when either is expressed alone. Moreover, co-expression of P{Sym-UAS-Hsrω} in the Scer\GAL4Act5C.PI, nejdsRNA.Scer\UAS.cKa background results in eclosing pupae, whereas no pupae eclose when P{Sym-UAS-Hsrω} is expressed alone. Co-expression of P{Sym-UAS-Hsrω} suppresses the Scer\GAL4GMR.PF, nejΔNZK.Scer\UAS eye phenotype, and results in near wild type eye morphology. Flies co-expressing Pros261.B.Scer\UAS and Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 25[o]C appear near normal. Additional co-expression of P{Sym-UAS-Hsrω} does not alter the external eye morphology. However, the pseudopupil images of flies expressing Pros261.B.Scer\UAS and Prosβ21.Scer\UAS reveal severe retinal damage, which is substantially rescued by additional co-expression of one copy of P{Sym-UAS-Hsrω}.
Scer\GAL4GMR.PF-mediated expression of P{Sym-UAS-Hsrω} significantly rescues the rprGMR.PW or grimGMR.PH eye phenotypes, but only marginally rescues the WGMR.PG eye phenotype. Scer\GAL4GMR.PF-mediated expression of P{Sym-UAS-Hsrω} eliminates the enhancing effect of HsrωEP3037 on rprGMR.PW or grimGMR.PH eyes as well as reversing the level of degeneration seen in eyes expressing rprGMR.PW or grimGMR.PH alone. Co-expression of P{Sym-UAS-Hsrω} mitigates the eye phenotypes seen in flies expressing either the weak or strong Ncpro.Scer\UAS under the control of Scer\GAL4GMR.PF. Co-expression of P{Sym-UAS-Hsrω} mitigates the eye phenotypes seen in Scer\GAL4GMR.PF, NcDeltaN.Scer\UAS flies. Co-expression of P{Sym-UAS-Hsrω} rescues the pupal lethality and small head phenotypes seen in flies expressing the strong Ncpro.Scer\UAS or NcDeltaN.Scer\UAS under the control of Scer\GAL4GMR.PF. Expression of P{Sym-UAS-Hsrω} leads to recovery of eye size and morphology in Scer\GAL4GMR.PF,thdsRNA.Scer\UAS.cLa flies, though the ommatidial lattice is still disrupted. Pupal lethality is also suppressed. Co-expression of P{Sym-UAS-Hsrω} in the rprGMR.PW, thScer\UAS.cHa, Scer\GAL4GMR.PF background improves eye morphology such that eyes are indistinguishable from wild type. Co-expression of P{Sym-UAS-Hsrω} fails to suppress the eye damage in the rprGMR.PW, thdsRNA.Scer\UAS.cLa, Scer\GAL4GMR.PF background. Co-expression of P{Sym-UAS-Hsrω} fails to suppress the eye damage in the grimGMR.PH, thdsRNA.Scer\UAS.cLa, Scer\GAL4GMR.PF background. Co-expression of P{Sym-UAS-Hsrω} restores ommatidial integrity of Scer\GAL4GMR.PF, egrScer\UAS.cIa almost to wild type, and also substantially reduces the mortality of differentiated pupae. Co-expression of P{Sym-UAS-Hsrω} suppresses the eye phenotype of Scer\GAL4GMR.PF, Tak1Scer\UAS.cTa, and also substantially reduces the mortality of differentiated pupae. Co-expression of P{Sym-UAS-Hsrω} strongly suppresses the severe eye degeneration in Scer\GAL4GMR.PF, egrScer\UAS.cIa, pucE69/+ flies, restoring ommatidial integrity and eye size to almost that of wild type. Co-expression of P{Sym-UAS-Hsrω} suppresses the photoreceptor degeneration phenotype seen in Scer\GAL4GMR.PF, egrScer\UAS.cIa eye discs. Additional co-expression of P{Sym-UAS-Hsrω} in Scer\GAL4GMR.PF, egrScer\UAS.cIa, thScer\UAS.cHa flies restores eyes to wild type. However, additional co-expression of P{Sym-UAS-Hsrω} in Scer\GAL4GMR.PF, egrScer\UAS.cIa, thdsRNA.Scer\UAS.cLa flies fails to rescue the eye phenotype. Co-expression of P{Sym-UAS-Hsrω} suppresses the optic stalk axonal projection phenotype seen in Scer\GAL4GMR.PF, egrScer\UAS.cIa eye discs. Co-expression of P{Sym-UAS-Hsrω} restores wing morphology to Scer\GAL4vg.PM, egrScer\UAS.cIa wings such that they are indistinguishable from wild type.
Xenogenetic Interactions
Statement
Reference
The eye degeneration and the associated rough eye appearance, ommatidial fusion and pigmentation loss characteristic for adult flies expressing Hsap\FUSScer\UAS.cIa under the control of Scer\GAL4GMR.PS is completely rescued by co-expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS. The complete rescue of the eye defects by lncRNA:HsrωdsRNA.Sym.Scer\UAS expression is however prevented by combination with a single copy of Lamp1MI15062 as a vast majority of Lamp1MI15062 heterozygotes expressing both Hsap\FUSScer\UAS.cIa and lncRNA:HsrωdsRNA.Sym.Scer\UAS under Scer\GAL4GMR.PS display areas of degeneration in the eyes. The eye degeneration induced by Hsap\FUSScer\UAS.cIa expression (one copy) under Scer\GAL4GMR.PS is not exacerbated by combination with Atg8ad4/+ and the eye defects in the Scer\GAL4GMR.PS/Atg8ad4;Hsap\FUSScer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS (one copy). The extreme eye degeneration phenotype (complete loss of the ommatidial lattice), observed when two copies of Hsap\FUSScer\UAS.cIa are expressed and the flies kept at 28[o]C, is rather weakly suppressed by expression of one copy of lncRNA:HsrωdsRNA.Sym.Scer\UAS and more strongly when two copies are used but even then the rescue remains incomplete under these conditions. The eye degeneration induced by Hsap\FUSScer\UAS.cIa expression (one copy) is not exacerbated by combination with Atg8ad4/+ and the eye defects in the Scer\GAL4GMR.PS/Atg8ad4;Hsap\FUSScer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS (one copy). The increase in the number of apoptotic ([*]Casp3-positive) cells in third instar larval eye discs observed upon Scer\GAL4GMR.PS-expression of Hsap\FUSScer\UAS.cIa is partially suppressed by co-expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS. The mild rough eye phenotype observed in flies carrying the Scer\GAL4GMR.PS driver insertion (without any responder UAS transgene) is ameliorated by expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS. This rescue is however prevented by combination with a single copy of Lamp1MI15062 as Lamp1MI15062/+ heterozygous flies expressing lncRNA:HsrωdsRNA.Sym.Scer\UAS under the control of Scer\GAL4GMR.PS display severe eye morphological defects.
The rough eye phenotype seen in the absence of any responder transgene in animals carrying two copies of Scer\GAL4GMR.PU is suppressed in a dose-dependent manner by the addition of lncRNA:HsrωdsRNA.Sym.UAS.
Inclusion of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and substantially reduces the mortality seen in flies expressing Scer\GAL4GMR.PF-driven Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in a Df(3R)Hrb87F/+ background. Expression of nejF2161A.Scer\UAS.T:SV5\V5 in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 background results in 23% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and size and reduces pupal death. Expression of nejF2161A.Scer\UAS.T:SV5\V5 in the Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 background results in 15% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and size and reduces pupal death. Expression of nejdsRNA.Scer\UAS.cKa in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 background results in >97% lethality at the pharate stage with eye degeneration being dramatically enhanced. Additional co-expression of P{Sym-UAS-Hsrω} robustly suppresses this enhanced eye damage, and also substantially reverses Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 eye degeneration in a dose-dependent manner. Co-expression of P{Sym-UAS-Hsrω} also improves emergence to adult stage in a dose-dependent manner. Expression of nejdsRNA.Scer\UAS.cKa in the Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 background results in >97% lethality at the pharate stage with eye degeneration being dramatically enhanced. Additional co-expression of P{Sym-UAS-Hsrω} robustly suppresses this enhanced eye damage, and also substantially reverses Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 eye degeneration in a dose-dependent manner. Co-expression of P{Sym-UAS-Hsrω} also improves emergence to adult stage in a dose-dependent manner. The eye damage caused by Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is suppressed by co-expression of one copy of P{Sym-UAS-Hsrω}. Co-expression of P{Sym-UAS-Hsrω} still suppresses the damage in Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS eyes, but the eyes are not rescued to the degree seen in Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 eyes. The increase in inclusion bodies in larval eye discs caused by Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is reduced by co-expression of one copy of P{Sym-UAS-Hsrω}. However, co-expression of P{Sym-UAS-Hsrω} fails to reduce the number of inclusion bodies in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS background. The damaged eye phenotype that results from Scer\GAL4GMR.PF-mediated expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 is suppressed by co-expression of P{Sym-UAS-Hsrω}. Co-expression of P{Sym-UAS-Hsrω} still suppresses the damage in Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS eyes, but the eyes are not rescued to the degree seen in Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 eyes.
Expression of two copies of P{Sym-UAS-Hsrω} suppresses the rough eye, photoreceptor degeneration, and increased apoptosis phenotypes seen in Scer\GAL4GMR.PF/Scer\GAL4GMR.PF flies.
Co-expression of two copies of P{Sym-UAS-Hsrω} nearly completely suppresses the Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF eye phenotype in 54% of flies; photoreceptor neurons show improved morphology with the majority of ommatidia showing the normal seven rhabdomeres. Co-expression of one copy of P{Sym-UAS-Hsrω} also mitigates the damage caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, but to lesser extent. Co-expression of a single copy of P{Sym-UAS-Hsrω} eliminates the enhancing effect of HsrωEP3037 or HsrωEP93D expression on the Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF eye phenotype, and also substantially reverses the eye degeneration primarily induced by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1. Co-expression of two copies of P{Sym-UAS-Hsrω} restores pigmentation and reduces the roughening of Hsap\ATX182Q.Scer\UAS, Scer\GAL4GMR.PF eyes. Co-expression of two copies of P{Sym-UAS-Hsrω} almost completely suppresses the Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF eye phenotype: the external appearance is wild type but rhabdomere arrays are still abnormal. Co-expression of two copies of P{Sym-UAS-Hsrω} substantially inhibits the age dependant degeneration of rhabdomeres seen in Hsap\HDQ93.ex1p.Scer\UAS, Scer\GAL4GMR.PF flies. Co-expression of one or two copies of P{Sym-UAS-Hsrω} restores normal phototactic behaviour to: i) Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF flies; ii) Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF flies; and iii) Hsap\HDQ93.ex1p.Scer\UAS, Scer\GAL4GMR.PF flies. Co-expression of a single copy of P{Sym-UAS-Hsrω} in the Scer\GAL4elav-C155, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 background increases the proportion of flies surviving to adulthood from 0% to 98%. Co-expression of a single copy of P{Sym-UAS-Hsrω} rescues the pharate lethality seen in Scer\GAL4elav-C155, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 flies, allowing 94% of pupae to eclose. However, surviving female flies display abdominal swellings, and lifespan is reduced compared to controls. Co-expression of a single copy of P{Sym-UAS-Hsrω} significantly lowers the proportion of Scer\GAL4elav-C155, Hsap\ATX182Q.Scer\UAS flies dying at each stage of development, such that a greater fraction die as differentiated pharates. Co-expression of a single copy of P{Sym-UAS-Hsrω} prolongs the lifespan of Scer\GAL4elav-C155, Hsap\HDQ93.ex1p.Scer\UAS flies such that it becomes comparable with controls.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Associated with: Hsrω[a.Sym.Scer\UAS]
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
HsrωSym.Scer\UAS
HsrωdsRNA.Sym.Scer\UAS
lncRNA:HsrωdsRNA.Sym.Scer\UAS
lncRNA:HsrωdsRNA.Sym.UAS
Name Synonyms
Secondary FlyBase IDs
  • FBal0243043
References (8)