Co-expression of Ras85D^V12.UAS with lncRNA:Hsrω^{dsRNA.Sym.UAS} under the control of Scer\GAL4^GMR.PF leads to following changes during pupal development: complete absence of proper demarcation of head, thorax, and abdomen; and absence of retraction of Malpighian tubules (MT) to become abdominal.

In the early dying 24- to 25-hour-old lncRNA:Hsrω^{dsRNA.Sym.UAS} and Ras85D^V12.UAS co-expressing pupae under the control of Scer\GAL4^sev.EP the metamorphic changes causing compaction of cells in MT, the histolysis of salivary gland, or the disappearance of the posterior seven pairs of abdominal segmental dorsomedian UAS-GFP expressing neurons under the control of Scer\GAL4^sev.EP do not occur. These pupae continue to show those structures as in 8- to 9-hour-old pupae even at 24 to 25 hours APF.

Third instar larvae simultaneously expressing both Hsrω^{dsRNA.Sym.Scer\UAS} and caz^VDRC.cUa under the control of Scer\GAL4^elav.PLu display crawling ability deficiency as well as morphological defects in the neuromuscular junctions (reduced total synaptic branch length and number of boutons) but the severity of each of these defects is not significantly different from either of the single knockdowns.

The eye morphology defects (rough appearance with fused ommatidia) characteristic for flies expressing caz^VDRC.cUa under the control of Scer\GAL4^GMR.PS can be rescued by co-expression of Hsrω^{dsRNA.Sym.Scer\UAS}.

Expression of LamC^UAS.cGa and lncRNA:Hsrω^{dsRNA.Sym.UAS} under the control of Scer\GAL4^Act5C.PI delays the lethality seen with LamC^UAS.cGa/Scer\GAL4^Act5C.PI, so that most die as pupae or pharate adults; the majority of pharates show a severe head capsule defect, with loss of most head structures; the few adults that emerge are all female.

Expression of Hsrω^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^ey.PH suppresses the defects seen in homozygous Iswi² eyes.

Expression of Hsrω^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^ey.PH suppresses the X chromosome condensation defects seen in the polytene chromosomes of Iswi¹/Iswi² male larvae.

Co-expression of Hsrω^{dsRNA.Sym.Scer\UAS} suppresses the mutant eye and polytene chromosome phenotypes caused by expression of Iswi^{K159R.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH.

Coexpression of one copy of P{Sym-UAS-Hsrω} in the Scer\GAL4^GMR.PF, nej^{F2161A.Scer\UAS.T:SV5\V5} background substantially improves eye morphology and ommatidial arrays.

Co-expression of P{Sym-UAS-Hsrω} in the Scer\GAL4^GMR.PF, nej^{dsRNA.Scer\UAS.cKa} background significantly improves pigmentation and eye morphology, including in the posterior part.

More embryos/larvae die when nej^{dsRNA.Scer\UAS.cKa} and P{Sym-UAS-Hsrω} are co-expressed with Scer\GAL4^Act5C.PI than when either is expressed alone. Moreover, co-expression of P{Sym-UAS-Hsrω} in the Scer\GAL4^Act5C.PI, nej^{dsRNA.Scer\UAS.cKa} background results in eclosing pupae, whereas no pupae eclose when P{Sym-UAS-Hsrω} is expressed alone.

Co-expression of P{Sym-UAS-Hsrω} suppresses the Scer\GAL4^GMR.PF, nej^{ΔNZK.Scer\UAS} eye phenotype, and results in near wild type eye morphology.

Flies co-expressing Pros26^1.B.Scer\UAS and Prosβ2^1.Scer\UAS under the control of Scer\GAL4^GMR.PF at 25[o]C appear near normal. Additional co-expression of P{Sym-UAS-Hsrω} does not alter the external eye morphology. However, the pseudopupil images of flies expressing Pros26^1.B.Scer\UAS and Prosβ2^1.Scer\UAS reveal severe retinal damage, which is substantially rescued by additional co-expression of one copy of P{Sym-UAS-Hsrω}.

Scer\GAL4^GMR.PF-mediated expression of P{Sym-UAS-Hsrω} significantly rescues the rpr^GMR.PW or grim^GMR.PH eye phenotypes, but only marginally rescues the W^GMR.PG eye phenotype.

Scer\GAL4^GMR.PF-mediated expression of P{Sym-UAS-Hsrω} eliminates the enhancing effect of Hsrω^EP3037 on rpr^GMR.PW or grim^GMR.PH eyes as well as reversing the level of degeneration seen in eyes expressing rpr^GMR.PW or grim^GMR.PH alone.

Co-expression of P{Sym-UAS-Hsrω} mitigates the eye phenotypes seen in flies expressing either the weak or strong Nc^pro.Scer\UAS under the control of Scer\GAL4^GMR.PF.

Co-expression of P{Sym-UAS-Hsrω} mitigates the eye phenotypes seen in Scer\GAL4^GMR.PF, Nc^{DeltaN.Scer\UAS} flies.

Co-expression of P{Sym-UAS-Hsrω} rescues the pupal lethality and small head phenotypes seen in flies expressing the strong Nc^pro.Scer\UAS or Nc^{DeltaN.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

Expression of P{Sym-UAS-Hsrω} leads to recovery of eye size and morphology in Scer\GAL4^GMR.PF,th^{dsRNA.Scer\UAS.cLa} flies, though the ommatidial lattice is still disrupted. Pupal lethality is also suppressed.

Co-expression of P{Sym-UAS-Hsrω} in the rpr^GMR.PW, th^Scer\UAS.cHa, Scer\GAL4^GMR.PF background improves eye morphology such that eyes are indistinguishable from wild type.

Co-expression of P{Sym-UAS-Hsrω} fails to suppress the eye damage in the rpr^GMR.PW, th^{dsRNA.Scer\UAS.cLa}, Scer\GAL4^GMR.PF background.

Co-expression of P{Sym-UAS-Hsrω} fails to suppress the eye damage in the grim^GMR.PH, th^{dsRNA.Scer\UAS.cLa}, Scer\GAL4^GMR.PF background.

Co-expression of P{Sym-UAS-Hsrω} restores ommatidial integrity of Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa almost to wild type, and also substantially reduces the mortality of differentiated pupae.

Co-expression of P{Sym-UAS-Hsrω} suppresses the eye phenotype of Scer\GAL4^GMR.PF, Tak1^Scer\UAS.cTa, and also substantially reduces the mortality of differentiated pupae.

Co-expression of P{Sym-UAS-Hsrω} strongly suppresses the severe eye degeneration in Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa, puc^E69/+ flies, restoring ommatidial integrity and eye size to almost that of wild type.

Co-expression of P{Sym-UAS-Hsrω} suppresses the photoreceptor degeneration phenotype seen in Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa eye discs.

Additional co-expression of P{Sym-UAS-Hsrω} in Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa, th^Scer\UAS.cHa flies restores eyes to wild type. However, additional co-expression of P{Sym-UAS-Hsrω} in Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa, th^{dsRNA.Scer\UAS.cLa} flies fails to rescue the eye phenotype.

Co-expression of P{Sym-UAS-Hsrω} suppresses the optic stalk axonal projection phenotype seen in Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa eye discs.

Co-expression of P{Sym-UAS-Hsrω} restores wing morphology to Scer\GAL4^vg.PM, egr^Scer\UAS.cIa wings such that they are indistinguishable from wild type.

The eye degeneration and the associated rough eye appearance, ommatidial fusion and pigmentation loss characteristic for adult flies expressing Hsap\FUS^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PS is completely rescued by co-expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS}. The complete rescue of the eye defects by lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} expression is however prevented by combination with a single copy of Lamp1^MI15062 as a vast majority of Lamp1^MI15062 heterozygotes expressing both Hsap\FUS^Scer\UAS.cIa and lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} under Scer\GAL4^GMR.PS display areas of degeneration in the eyes.

The eye degeneration induced by Hsap\FUS^Scer\UAS.cIa expression (one copy) under Scer\GAL4^GMR.PS is not exacerbated by combination with Atg8a^d4/+ and the eye defects in the Scer\GAL4^GMR.PS/Atg8a^d4;Hsap\FUS^Scer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} (one copy).

The extreme eye degeneration phenotype (complete loss of the ommatidial lattice), observed when two copies of Hsap\FUS^Scer\UAS.cIa are expressed and the flies kept at 28[o]C, is rather weakly suppressed by expression of one copy of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} and more strongly when two copies are used but even then the rescue remains incomplete under these conditions.

The eye degeneration induced by Hsap\FUS^Scer\UAS.cIa expression (one copy) is not exacerbated by combination with Atg8a^d4/+ and the eye defects in the Scer\GAL4^GMR.PS/Atg8a^d4;Hsap\FUS^Scer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} (one copy).

The increase in the number of apoptotic ([*]Casp3-positive) cells in third instar larval eye discs observed upon Scer\GAL4^GMR.PS-expression of Hsap\FUS^Scer\UAS.cIa is partially suppressed by co-expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS}.

The mild rough eye phenotype observed in flies carrying the Scer\GAL4^GMR.PS driver insertion (without any responder UAS transgene) is ameliorated by expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS}. This rescue is however prevented by combination with a single copy of Lamp1^MI15062 as Lamp1^MI15062/+ heterozygous flies expressing lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^GMR.PS display severe eye morphological defects.

The rough eye phenotype seen in the absence of any responder transgene in animals carrying two copies of Scer\GAL4^GMR.PU is suppressed in a dose-dependent manner by the addition of lncRNA:Hsrω^{dsRNA.Sym.UAS}.

Inclusion of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and substantially reduces the mortality seen in flies expressing Scer\GAL4^GMR.PF-driven Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} in a Df(3R)Hrb87F/+ background.

Expression of nej^{F2161A.Scer\UAS.T:SV5\V5} in the Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} background results in 23% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and size and reduces pupal death.

Expression of nej^{F2161A.Scer\UAS.T:SV5\V5} in the Scer\GAL4^GMR.PF, Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} background results in 15% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of one copy of P{Sym-UAS-Hsrω} improves the eye morphology and size and reduces pupal death.

Expression of nej^{dsRNA.Scer\UAS.cKa} in the Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} background results in >97% lethality at the pharate stage with eye degeneration being dramatically enhanced. Additional co-expression of P{Sym-UAS-Hsrω} robustly suppresses this enhanced eye damage, and also substantially reverses Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} eye degeneration in a dose-dependent manner. Co-expression of P{Sym-UAS-Hsrω} also improves emergence to adult stage in a dose-dependent manner.

Expression of nej^{dsRNA.Scer\UAS.cKa} in the Scer\GAL4^GMR.PF, Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} background results in >97% lethality at the pharate stage with eye degeneration being dramatically enhanced. Additional co-expression of P{Sym-UAS-Hsrω} robustly suppresses this enhanced eye damage, and also substantially reverses Scer\GAL4^GMR.PF, Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} eye degeneration in a dose-dependent manner. Co-expression of P{Sym-UAS-Hsrω} also improves emergence to adult stage in a dose-dependent manner.

The eye damage caused by Scer\GAL4^GMR.PF-mediated expression of Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} is suppressed by co-expression of one copy of P{Sym-UAS-Hsrω}. Co-expression of P{Sym-UAS-Hsrω} still suppresses the damage in Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}, Pros26^1.B.Scer\UAS, Prosβ2^1.Scer\UAS eyes, but the eyes are not rescued to the degree seen in Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} eyes.

The increase in inclusion bodies in larval eye discs caused by Scer\GAL4^GMR.PF-mediated expression of Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} is reduced by co-expression of one copy of P{Sym-UAS-Hsrω}. However, co-expression of P{Sym-UAS-Hsrω} fails to reduce the number of inclusion bodies in the Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}, Pros26^1.B.Scer\UAS, Prosβ2^1.Scer\UAS background.

The damaged eye phenotype that results from Scer\GAL4^GMR.PF-mediated expression of Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} is suppressed by co-expression of P{Sym-UAS-Hsrω}. Co-expression of P{Sym-UAS-Hsrω} still suppresses the damage in Scer\GAL4^GMR.PF, Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Pros26^1.B.Scer\UAS, Prosβ2^1.Scer\UAS eyes, but the eyes are not rescued to the degree seen in Scer\GAL4^GMR.PF, Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} eyes.

Expression of two copies of P{Sym-UAS-Hsrω} suppresses the rough eye, photoreceptor degeneration, and increased apoptosis phenotypes seen in Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF flies.

Co-expression of two copies of P{Sym-UAS-Hsrω} nearly completely suppresses the Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF eye phenotype in 54% of flies; photoreceptor neurons show improved morphology with the majority of ommatidia showing the normal seven rhabdomeres. Co-expression of one copy of P{Sym-UAS-Hsrω} also mitigates the damage caused by Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}, but to lesser extent.

Co-expression of a single copy of P{Sym-UAS-Hsrω} eliminates the enhancing effect of Hsrω^EP3037 or Hsrω^EP93D expression on the Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF eye phenotype, and also substantially reverses the eye degeneration primarily induced by Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}.

Co-expression of two copies of P{Sym-UAS-Hsrω} restores pigmentation and reduces the roughening of Hsap\ATX1^82Q.Scer\UAS, Scer\GAL4^GMR.PF eyes.

Co-expression of two copies of P{Sym-UAS-Hsrω} almost completely suppresses the Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF eye phenotype: the external appearance is wild type but rhabdomere arrays are still abnormal.

Co-expression of two copies of P{Sym-UAS-Hsrω} substantially inhibits the age dependant degeneration of rhabdomeres seen in Hsap\HD^{Q93.ex1p.Scer\UAS}, Scer\GAL4^GMR.PF flies.

Co-expression of one or two copies of P{Sym-UAS-Hsrω} restores normal phototactic behaviour to: i) Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF flies; ii) Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF flies; and iii) Hsap\HD^{Q93.ex1p.Scer\UAS}, Scer\GAL4^GMR.PF flies.

Co-expression of a single copy of P{Sym-UAS-Hsrω} in the Scer\GAL4^elav-C155, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} background increases the proportion of flies surviving to adulthood from 0% to 98%.

Co-expression of a single copy of P{Sym-UAS-Hsrω} rescues the pharate lethality seen in Scer\GAL4^elav-C155, Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} flies, allowing 94% of pupae to eclose. However, surviving female flies display abdominal swellings, and lifespan is reduced compared to controls.

Co-expression of a single copy of P{Sym-UAS-Hsrω} significantly lowers the proportion of Scer\GAL4^elav-C155, Hsap\ATX1^82Q.Scer\UAS flies dying at each stage of development, such that a greater fraction die as differentiated pharates.

Co-expression of a single copy of P{Sym-UAS-Hsrω} prolongs the lifespan of Scer\GAL4^elav-C155, Hsap\HD^{Q93.ex1p.Scer\UAS} flies such that it becomes comparable with controls.