Allele Dmel\nes^Δ52

General Information
Symbol	Dmel\nes^Δ52	Species	D. melanogaster
Name		FlyBase ID	FBal0243176
Feature type	allele	Associated gene	Dmel\nes

Map ( GBrowse )
Allele class
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Steinhauer et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References deletion 3L:19,251,881..19,253,741 comment=Approximate endpoints of the 1.86 kb deletion resulting from the imprecise excision of P{EPgy2}nes[EY22898]. Position of deletion on reference sequence inferred by FlyBase curator. (Steinhauer et al., 2009)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	nes^EY22898 (Steinhauer et al., 2009)
Nature of the lesion	Statement Reference Imprecise excision of P{EPgy2}nes[EY22898] generates a 1.9kb deletion that removes most of the open reading frame. (Steinhauer et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference nes[Δ52] mutant flies survive to adulthood with no apparent morphological defects. (Steinhauer et al., 2009)
External Data
Linkouts
Interactions
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Phenotypic Class
Other
Statement Reference nes^Δ52, oys^Δ67 has female semi-sterile phenotype (Steinhauer et al., 2009) nes^Δ52, oys^Δ67 has male sterile phenotype (Steinhauer et al., 2009)
Phenotype Manifest In
Other
Statement Reference nes^Δ52, oys^Δ67 has individualization stage spermatid phenotype (Steinhauer et al., 2009) nes^Δ52, oys^Δ67 has spermatozoon phenotype (Steinhauer et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference oys[Δ67];nes[Δ52] double mutant males are sterile. Female fertility is also reduced; 70% of embryos resulting from a cross to heterozygous males fail to hatch and 24% display extensive degeneration of cell membranes. Removal of zygotic oys and nes further reduces adult viability to 10% of the number of flies carrying wild-type paternal copies of both genes. oys[Δ67];nes[Δ52] mutant testes appear relatively normal by light and phase-contrast microscopy and contain elongated spermatids. The early stages of spermatogenesis proceed normally in oys[Δ67];nes[Δ52] mutants. oys[Δ67];nes[Δ52] onion-stage spermatids contain individual Nebenkerne of normal size, indicating normal cytokinesis. Axonemes exhibit no defects in elongation and major and minor mitochondrial derivatives in pre-individualized spermatids display normal morphology. As no mature motile sperm are found in mutant seminal vesicles, oys and nes must act in the final stages of spermatid differentiation and maturation. Individualization complexes (ICs) are visible in oys[Δ67];nes[Δ52] double mutants in the basal region of the testis, in association with the cyst nuclei. However, very few progressing ICs are detectable and no ICs collect in the apical testis. Actin cones assemble normally around the spermatid nuclei, but dissociate away from each other as they move away from the nuclei, disassembling the IC. Intact ICs are almost never found apical to the nuclei, and individual stray actin cones are frequently observed. Embryos laid by oys[Δ67];nes[Δ52] mutant mothers contain a significant number of germ cells that fail to reach the gonads. These germ cells appear to migrate through the midgut epithelium normally, but they remain associated with the basal surface of the gut rather than attaching to the somatic gonadal precursors or scattering throughout the embryo. This phenotype is not observed in single mutants or controls. (Steinhauer et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	nes^Δ52 (Steinhauer et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Steinhauer et al., 2009, Mol. Biol. Cell 20(24): 5224--5235 Drosophila Lysophospholipid Acyltransferases Are Specifically Required for Germ Cell Development. [FBrf0209500]

Allele Dmel\nesΔ52

Allele Dmel\nes^Δ52