|Feature type||allele||Associated gene||Dmel\oys|
|Map ( GBrowse )|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Phenotype Manifest In|
|Phenotype Manifest In|
|NOT Enhanced by|
|NOT suppressed by|
oys[Δ67];nes[Δ52] double mutant males are sterile. Female fertility is also reduced; 70% of embryos resulting from a cross to heterozygous males fail to hatch and 24% display extensive degeneration of cell membranes. Removal of zygotic oys and nes further reduces adult viability to 10% of the number of flies carrying wild-type paternal copies of both genes. oys[Δ67];nes[Δ52] mutant testes appear relatively normal by light and phase-contrast microscopy and contain elongated spermatids. The early stages of spermatogenesis proceed normally in oys[Δ67];nes[Δ52] mutants. oys[Δ67];nes[Δ52] onion-stage spermatids contain individual Nebenkerne of normal size, indicating normal cytokinesis. Axonemes exhibit no defects in elongation and major and minor mitochondrial derivatives in pre-individualized spermatids display normal morphology. As no mature motile sperm are found in mutant seminal vesicles, oys and nes must act in the final stages of spermatid differentiation and maturation. Individualization complexes (ICs) are visible in oys[Δ67];nes[Δ52] double mutants in the basal region of the testis, in association with the cyst nuclei. However, very few progressing ICs are detectable and no ICs collect in the apical testis. Actin cones assemble normally around the spermatid nuclei, but dissociate away from each other as they move away from the nuclei, disassembling the IC. Intact ICs are almost never found apical to the nuclei, and individual stray actin cones are frequently observed. Embryos laid by oys[Δ67];nes[Δ52] mutant mothers contain a significant number of germ cells that fail to reach the gonads. These germ cells appear to migrate through the midgut epithelium normally, but they remain associated with the basal surface of the gut rather than attaching to the somatic gonadal precursors or scattering throughout the embryo. This phenotype is not observed in single mutants or controls. frj[Δ30], oys[Δ67];nes[Δ17]/nes[Δ80] triple mutants are identical to oys[Δ67];nes[Δ17]/nes[Δ80] double mutants.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 1 )|