Allele Hsap\APP^{Aβ1-42.Scer\UAS.Arc}

General Information
Symbol	Hsap\APP^{Aβ1-42.Scer\UAS.Arc}	Species	H. sapiens
Name		FlyBase ID	FBal0243229
Feature type	allele	Associated gene	Hsap\APP

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - site directed mutagenesis
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Iijima et al., 2008) in vitro construct - regulatory fusion (Iijima et al., 2008) in vitro construct - site directed mutagenesis (Iijima et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an E22G ("Arctic") mutant of the Aβ1-42 peptide of Hsap\APP with an N-terminal signal sequence to target it to the secretory pathway. (Iijima et al., 2008)
Carried in construct	P{UAS-Aβ42.Arc} (Iijima et al., 2008, Diangelo et al., 2011, Iijima-Ando et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor behavior defective, with Scer\GAL4^elav-C155 (Iijima et al., 2008) memory defective, with Scer\GAL4^elav-C155 (Iijima et al., 2008) short lived, with Scer\GAL4^elav-C155 (Iijima et al., 2008) visible \| adult stage, with Scer\GAL4^GMR.PF (Iijima-Ando et al., 2010)
Phenotype Manifest In
	calyx of adult mushroom body, with Scer\GAL4^ey-OK107 (Iijima et al., 2008) eye, with Scer\GAL4^GMR.PF (Iijima-Ando et al., 2010) neuron, with Scer\GAL4^elav-C155 (Iijima et al., 2008) neuropil, with Scer\GAL4^elav-C155 (Iijima et al., 2008) neuropil, with Scer\GAL4^ey-OK107 (Iijima et al., 2008)
Detailed Description
	Statement Reference Expression of Hsap\APP[Aβ1-42.Scer\UAS.Arc] in the central clock neurons under the control of Scer\GAL4[P2.4.Pdf] elevates the levels of fat body triglycerides compared to controls. Circadian behavior appears normal. These mutants do not consume more food over a 24hr period compared to controls. (Diangelo et al., 2011) Expression of Hsap\APP[Aβ1-42.Scer\UAS.Arc] in the eye under the control of Scer\GAL4[GMR.PF] causes degeneration. (Iijima-Ando et al., 2010) Expression of Hsap\APP[Aβ1-42.Scer\UAS.Arc] driven by Scer\GAL4[elav-C155] results in neurodegeneration, locomotor dysfunction and decreased adult lifespan. 5 and 10 day old mutant flies also manifest 1 hour memory defects. Scer\GAL4[elav-C155]- or Scer\GAL4[ey-OK107]-driven expression of Hsap\APP[Aβ1-42.Scer\UAS.Arc] results in neuropil degeneration in flies 79 days after eclosion. Calyxes in the adult mushroom body expressing Scer\GAL4[ey-OK107]-driven Hsap\APP[Aβ1-42.Scer\UAS.Arc] start to degenerate 79 days after eclosion. (Iijima et al., 2008)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Enhanced by
Statement Reference Hsap\APP^{Aβ1-42.Scer\UAS.Arc}, Scer\GAL4^GMR.PF has visible \| adult stage phenotype, non-enhanceable by lok^Scer\UAS.cPa, Scer\GAL4^GMR.PF (Iijima-Ando et al., 2010)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Hsap\APP^{Aβ1-42.Scer\UAS.Arc}, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by lok^Scer\UAS.cPa, Scer\GAL4^GMR.PF (Iijima-Ando et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of lok[Scer\UAS.cPa] does not enhance the eye degeneration phenotype seen when Hsap\APP[Aβ1-42.Scer\UAS.Arc] is expressed under the control of Scer\GAL4[GMR.PF]. (Iijima-Ando et al., 2010)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Hsap\APP^{Aβ1-42.Scer\UAS.Arc}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Diangelo et al., 2011, PLoS ONE 6(5): e19921 The central clock neurons regulate lipid storage in Drosophila. [FBrf0213791] Frydman-Marom et al., 2011, ACS Chem. Biol. 6(11): 1265--1276 Structural basis for inhibiting β-amyloid oligomerization by a non-coded β-breaker-substituted endomorphin analogue. [FBrf0218223] Iijima-Ando et al., 2010, Hum. Mol. Genet. 19(10): 1930--1938 A DNA damage-activated checkpoint kinase phosphorylates tau and enhances tau-induced neurodegeneration. [FBrf0210684] Iijima et al., 2008, PLoS ONE 3(2): e1703 Abeta42 mutants with different aggregation profiles induce distinct pathologies in Drosophila. [FBrf0210286]

Allele Hsap\APPAβ1-42.Scer\UAS.Arc

Allele Hsap\APP^{Aβ1-42.Scer\UAS.Arc}