Allele Dmel\mgl^{dsRNA.Scer\UAS.cKa}

General Information
Symbol	Dmel\mgl^{dsRNA.Scer\UAS.cKa}	Species	D. melanogaster
Name		FlyBase ID	FBal0243332
Feature type	allele	Associated gene	Dmel\mgl

Allele class
Mutagen	FLPase
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	FLPase (Khaliullina et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	mgl^{dsRNA.Scer\FRT.Scer\UAS.cKa} (Khaliullina et al., 2009)
Nature of the lesion	Statement Reference The Scer\FRT cassette has been excised, allowing expression of the inverted repeat of Megalin-RB nucleotides 11821-12342 from the Scer\UAS regulatory sequences. (Khaliullina et al., 2009)
Carried in construct	P{UAS(-FRT)mgl.dsRNA.K} (Riedel et al., 2011, Khaliullina et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	visible, with Scer\GAL4^C-765 (Riedel et al., 2011) visible, with Scer\GAL4^en-e16E (Riedel et al., 2011)
Phenotype Manifest In
	adult cuticle, with Scer\GAL4^C-765 (Riedel et al., 2011) procuticle, with Scer\GAL4^ap-md544 (Riedel et al., 2011) wing blade, with Scer\GAL4^C-765 (Riedel et al., 2011) wing blade \| posterior compartment, with Scer\GAL4^en-e16E (Riedel et al., 2011)
Detailed Description
	Statement Reference The cuticle of the adult wing blade is dull and opaque in flies expressing mgl[dsRNA.Scer\UAS.cKa] under the control of Scer\GAL4[C-765]. When mgl[dsRNA.Scer\UAS.cKa] is expressed under the control of Scer\GAL4[en-e16E], flies emerge with intact wings. However, after several days, the posterior wing blade appears frayed and broken, while the anterior part of the wing remains intact, suggesting that the cuticle secreted by cells expressing Scer\GAL4[en-e16E] is more fragile than normal. Expression of mgl[dsRNA.Scer\UAS.cKa] under the control of Scer\GAL4[ap-md544] does not perturb the formation of the outer envelope or epicuticle of the dorsal wing cuticle during pupal development. The thickness of the developing procuticle appears normal, but fissures in the distal procuticle are seen, which become more numerous with time, such that the procuticle of adult wings is dramatically cracked and split. (Riedel et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Megalin^{dsRNA.Scer\UAS} mgl^{dsRNA.Scer\UAS.cKa}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Riedel et al., 2011, Development 138(1): 149--158 Megalin-dependent Yellow endocytosis restricts melanization in the Drosophila cuticle. [FBrf0212536] Khaliullina et al., 2009, Development 136(24): 4111--4121 Patched regulates Smoothened trafficking using lipoprotein-derived lipids. [FBrf0209344]

Allele Dmel\mgldsRNA.Scer\UAS.cKa

Allele Dmel\mgl^{dsRNA.Scer\UAS.cKa}