Allele Dmel\cno^R2

General Information
Symbol	Dmel\cno^R2	Species	D. melanogaster
Name		FlyBase ID	FBal0243335
Feature type	allele	Associated gene	Dmel\cno

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Sawyer et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: K211@. (Sawyer et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| germline clone (Sawyer et al., 2009) planar polarity defective \| embryonic stage (Sawyer et al., 2009)
Phenotype Manifest In
	adherens junction (Sawyer et al., 2009) embryo \| dorsal closure stage \| germline clone (Sawyer et al., 2009) embryonic/first instar larval cuticle (Sawyer et al., 2009) head mesoderm (Sawyer et al., 2009) mesectoderm (Sawyer et al., 2009)
Detailed Description
	Statement Reference Zygotic cno[R2] mutant embryos die; 88% have defects in head involution but close dorsally, whereas 11% have defects in head involution and dorsal closure. Loss of maternal cno[R2] is not fully rescued by zygotic wild-type cno; ~30% of paternally rescued mutants die with defects in head involution. cno[R2] maternal+zygotic mutants are much more severe than zygotic mutants, which is consistent with strong maternal contribution. Most maternal/zygotic cno[R2] embryos (83%) entirely lack ventral cuticle, but retain dorsal cuticle. Initial adherens junctions in cno[R2] maternal and zygotic mutants is indistinguishable from wild-type. Maturation of spot adherens junctions to belt adherens junctions also proceeds normally. cno[R2] mutants do not completely internalize mesoderm during gastrulation (as in wild-type); many cells remain on the embryo surface and begin to divide in this aberrant location. While cno[R2] mutant mesodermal cells initiate constriction fairly synchronously, they arrest partway through apical constriction. The phenotype is variable. In less severe mutants, constriction goes at the same rate as in wild-type but halts prematurely. In more severe embryos, constriction is slower than in wild-type, and more cells lag behind. cno[R2] cells apically constrict without fully effective linkage between adherens junctions and the actomyosin network, the contractile network detaches from adherens junctions before full cell constriction, and mesodermal cells are not efficiently internalized. cno[R2] mutants successfully internalize the gut, although the midgut epithelium may be less organised. Ectodermal cells in cno[R2] mutants planar polarize myosin and adherens junction proteins in an even more pronounced manner than wild-type. They retain accentuated planar polarity through the end of germband extension. (Sawyer et al., 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	cno^R2 (Sawyer et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Sawyer et al., 2009, J. Cell Biol. 186(1): 57--73 The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction. [FBrf0208505]

Allele Dmel\cnoR2

Allele Dmel\cno^R2