Allele Rnor\CMR1^Scer\UAS.cPa

General Information
Symbol	Rnor\CMR1^Scer\UAS.cPa	Species	R. norvegicus
Name		FlyBase ID	FBal0243440
Feature type	allele	Associated gene	Rnor\CMR1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Peabody et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Rnor\CMR1 coding sequences. (Peabody et al., 2009)
Carried in construct	P{UAS-CMR1.P} (Peabody et al., 2009, Krashes et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	locomotor behavior defective \| cold sensitive, with Scer\GAL4^elav-C155 (Peabody et al., 2009) locomotor behavior defective \| conditional, with Scer\GAL4^elav-C155 (Peabody et al., 2009) memory defective \| heat sensitive, with Scer\GAL4^c061 (Krashes et al., 2009) memory defective \| heat sensitive, with Scer\GAL80^Mef2.PT (Krashes et al., 2009) neurophysiology defective \| cold sensitive, with Scer\GAL4^elav-C155 (Peabody et al., 2009)
Phenotype Manifest In
	wing \| conditional, with Scer\GAL4^Ccap.PP (Peabody et al., 2009) wing \| heat sensitive, with Scer\GAL4^Ccap.PP (Peabody et al., 2009)
Detailed Description
	Statement Reference Flies expressing Rnor\CMR1[Scer\UAS.cPa] under the control of Scer\GAL4[c061] and Scer\GAL80[Mef2.PT] do not show any differences in appetitive memory performance from controls at 23[o]C. However, shifting these flies to 16[o]C for 1 hour before testing impairs memory. (Krashes et al., 2009) Flies expressing Rnor\CMR1[Scer\UAS.cPa] pan-neuronally under the control of Scer\GAL4[elav-C155] fall down quickly upon transfer to 15[o]C, with animals expressing 3x Rnor\CMR1[Scer\UAS.cPa] displaying a more rapid and robust response. Electrophysiological recordings from flight muscles of these animals reveals sustained action potential generation in motoneurons in response to a downward temperature ramp. This effect is readily reversible. Males typically show greater sensitivity than females to a given temperature shift. Saturating menthol vapor administered to flies expressing Rnor\CMR1[Scer\UAS.cPa] pan-neuronally (under the control of Scer\GAL4[elav-C155]) at room temperature (24[o]C) causes the animals to seize and fall over as they do in response to cold. Flies expressing Rnor\CMR1[Scer\UAS.cPa] in N[[CCAP]] neurons under the control of Scer\GAL4[Ccap.PP] that are shifted briefly to 18[o]C immediately after eclosion rapidly expand their wings relative to controls. They respond the same way to a 15 minute exposure to saturating menthol vapor at room temperature. All flies exhibit an extremely brief grooming phase. Flies expressing Rnor\CMR1[Scer\UAS.cPa] under the control of Scer\GAL4[Ccap.PP] in high-perturbation conditions display normal cuticle tanning after N[[CCAP]] activation (indicating normal buriscon release). (Peabody et al., 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Rnor\CMR1^Scer\UAS.cPa
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Krashes et al., 2009, Cell 139(2): 416--427 A neural circuit mechanism integrating motivational state with memory expression in Drosophila. [FBrf0208897] Peabody et al., 2009, J. Neurosci. 29(11): 3343--3353 Characterization of the decision network for wing expansion in Drosophila using targeted expression of the TRPM8 channel. [FBrf0207505]

Allele Rnor\CMR1Scer\UAS.cPa

Allele Rnor\CMR1^Scer\UAS.cPa