FlyBase Allele Report: Dmel\Lasp[y45]

General Information

Symbol

Dmel\Lasp^y45

Species

D. melanogaster

Name

FlyBase ID

FBal0243456

Feature type

allele

Associated gene

Dmel\Lasp

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

hobo activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Suyama et al., 2009)

Mutagen

hobo activity

(Suyama et al., 2009)

Progenitor genotype

P{wHy}Lasp^DG14505

(Suyama et al., 2009)

Cytology

Description

Imprecise excision of the P{wHy}Lasp^DG14505 resulting in a 2859 bp deletion (with respect to the original insertion point of P{wHy}Lasp^DG14505).

(Suyama et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:16,679,583..16,682,441 [-]

Comment:

Reported as a deletion of 2859 bp from the site of the P{wHy}Lasp^DG14505 insertion at nt 16675541 (release 5.7).

(Suyama et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal hatching behavior

(Suyama et al., 2009)

decreased cell number | embryonic stage 11

(Suyama et al., 2009)

grandchildless

(Suyama et al., 2009)

partially lethal - majority die

(Suyama et al., 2009)

Phenotype Manifest In

primordial germ cell | embryonic stage 11

(Suyama et al., 2009)

Detailed Description

Statement

Reference

40-50% of Lasp^y45 mutant eggs fail to develop due to lack of fertilisation. The remaining embryos hatch and develop normally. Homozygous mutant Lasp^y45 females all contain normally developed ovaries.

The progeny of Lasp^y45/+ develop no germline (grandchildless phenotype) at very low penetrance.

An average of 26 pole cells are observed in Lasp^y45 mutant stage 11 embryos, slightly reduced compared to wildtype numbers (average of 33 pole cells).

(Suyama et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lasp^y45 has grandchildless | dominant phenotype, enhanceable by osk[+]/osk¹

(Suyama et al., 2009)

Suppressed by

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has grandchildless | dominant phenotype, suppressible by Scer\GAL4^oc.1.6/Lasp^L.UASp

(Suyama et al., 2009)

NOT suppressed by

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has grandchildless | dominant phenotype, non-suppressible by Scer\GAL4^oc.1.6/Lasp^WA.UASp

(Suyama et al., 2009)

Lasp^y41/Lasp^y45, osk¹ has grandchildless | dominant phenotype, non-suppressible by Lasp^S.UASp/Scer\GAL4^oc.1.6

(Suyama et al., 2009)

Enhancer of

Statement

Reference

Lasp^y45 is an enhancer of grandchildless | dominant phenotype of osk¹

(Suyama et al., 2009)

Lasp^y45 is an enhancer of decreased cell number | embryonic stage 11 phenotype of osk¹

(Suyama et al., 2009)

Other

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has grandchildless | dominant phenotype

(Suyama et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has primordial germ cell | embryonic stage 11 phenotype, suppressible by Scer\GAL4^oc.1.6/Lasp^L.UASp

(Suyama et al., 2009)

NOT suppressed by

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has primordial germ cell | embryonic stage 11 phenotype, non-suppressible by Scer\GAL4^oc.1.6/Lasp^WA.UASp

(Suyama et al., 2009)

Lasp^y41/Lasp^y45, osk¹ has primordial germ cell | embryonic stage 11 phenotype, non-suppressible by Lasp^S.UASp/Scer\GAL4^oc.1.6

(Suyama et al., 2009)

Enhancer of

Statement

Reference

Lasp^y45 is an enhancer of ovary phenotype of osk¹

(Suyama et al., 2009)

Lasp^y45 is an enhancer of primordial germ cell | embryonic stage 11 phenotype of osk¹

(Suyama et al., 2009)

Other

Statement

Reference

Lasp^y41/Lasp^y45, osk¹ has primordial germ cell | embryonic stage 11 phenotype

(Suyama et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

osk¹/+ females lacking both copies of Lasp^y45 show a penetrant grandchildless phenotype, with 20% of offspring displaying a complete absence of ovaries, and another 25% show only a single ovary.

(Suyama et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Lasp^y45 is rescued by Scer\GAL4^{VP16.mat.αTub67C}/Lasp^L.UASp

(Suyama et al., 2009)

Comments

Expression of Lasp^{L.Scer\UAS.P\T} restores the hatching defect in Lasp^y45 mutants.

Stage 11 embryos from osk¹/+ females lacking both copies of Lasp^y45 develop an average of 12 pole cells, much reduced compared to osk¹/+ animals.

Lasp^y45, osk¹/Lasp^y41 females have a grandchildless phenotype, which is rescued by expression of Lasp^{L.Scer\UAS.P\T} under the control of Scer\GAL4^otd. The number of pole cells is partially rescued, increasing to 25 pole cells in stage 11 embryos.

The grandchildless phenotype of Lasp^y45, osk¹/Lasp^y41 females is not rescued by expression of Lasp^{WA.Scer\UAS.P\T} or Lasp^{S.Scer\UAS.P\T} under the control of Scer\GAL4^otd. There is no increase in the number of pole cells observed in these embryos.

(Suyama et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lasp^y45

lasp^y45

(Suyama et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (1)

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