Flies expressing ShawRA.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4tim.PE (but not Scer\GAL4qsm-NP6258 or Scer\GAL4qsm-NP6258) exhibit ultradian locomotor rhythms in constant dim white light, in contrast to control flies which largely show arrhythmic behavior in these conditions. These flies do not exhibit any difference in locomotor rhythms when kept in constant darkness, as compared to controls.
The l-LNv neurons in flies expressing ShawRA.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Pdf.PU exhibit a hyperpolarized resting membrane potential, and a reduced spontaneous firing rate during the daytime, but not during the night, as compared to wild type; these neurons also fail to respond to blue light stimulus, in contrast to wild type.
Some adults expressing ShawRA.Scer\UAS.T:Zzzz\FLAG with Scer\GAL4Ccap.PP show a phenotype with unfurled wings, softened or abnormally tanned cuticle, and show an ether-induced shaking phenotype. Some small pupae fail to eclose.
Firing properties and whole cell parameters of MNISN-Is motor neurons are not substantially different from controls in animals expressing ShawRA.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4C380.