Allele Dmel\cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus}

General Information
Symbol	Dmel\cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus}	Species	D. melanogaster
Name		FlyBase ID	FBal0243515
Feature type	allele	Associated gene	Dmel\cln3

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Tuxworth et al., 2009) in vitro construct - regulatory fusion (Tuxworth et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of wild-type cln3 coding sequences tagged with T:Avic\GFP[YFP.Venus]. (Tuxworth et al., 2009)
Carried in construct	P{UAS-cln3.Venus} (Tuxworth et al., 2009, Tuxworth et al., 2011)
Tagged with	T:Avic\GFP^YFP.Venus (Tuxworth et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF (Tuxworth et al., 2009) ommatidium, with Scer\GAL4^GMR.PF (Tuxworth et al., 2009)
Detailed Description
	Statement Reference Expression of cln3[Scer\UAS.T:Avic\GFP-YFP.Venus] in the eye, under the control of Scer\GAL4[GMR.PF] results in misshapen and fused ommatidia. Scanning electron microscopy shows a loss of regular crystalline array of ommatidia, fusions of ommatidia and alterations in bristle patterns. Sections through the eye show extensive degeneration. (Tuxworth et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus} has eye phenotype, enhanceable by Df(2R)ED3791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Tuxworth et al., 2009) Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus} has ommatidium phenotype, enhanceable by Df(2R)ED3791, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Tuxworth et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference A Df(2R)ED3791 background enhances eye degeneration found in Scer\GAL4[GMR.PF]>cln3[Scer\UAS.T:Avic\GFP-YFP.Venus] flies. (Tuxworth et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus} rescues cln3^ΔMB1 (Tuxworth et al., 2011)
Comments	Ubiquitous expression of cln3[Scer\UAS.T:Avic\GFP-YFP.Venus] under the control of Scer\GAL4[arm.PS] rescues the hypersensitivity of cln3[ΔMB1] mutants to oxidative stress. The shortened lifespan of cln3[ΔMB1] homozygotes is also rescued by the expression of cln3[Scer\UAS.T:Avic\GFP-YFP.Venus] via Scer\GAL4[arm.PS]. (Tuxworth et al., 2011) A Df(3R)3-4 background suppresses eye degeneration found in Scer\GAL4[GMR.PF]>cln3[Scer\UAS.T:Avic\GFP-YFP.Venus] flies. (Tuxworth et al., 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Tuxworth et al., 2011, Hum. Mol. Genet. 20(10): 2037--2047 The Batten disease gene CLN3 is required for the response to oxidative stress. [FBrf0213533] Tuxworth et al., 2009, Hum. Mol. Genet. 18(4): 667--678 Interactions between the juvenile Batten disease gene, CLN3, and the Notch and JNK signalling pathways. [FBrf0206987]

Allele Dmel\cln3Scer\UAS.T:Avic\GFP-YFP.Venus

Allele Dmel\cln3^{Scer\UAS.T:Avic\GFP-YFP.Venus}