Allele Dmel\Slob^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\Slob^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0243530
Feature type	allele	Associated gene	Dmel\Slob

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Shahidullah et al., 2009) in vitro construct - RNAi (Shahidullah et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Two convergent arrays of Scer\UAS regulatory sequences drive the simultaneous transcription of both sense and antisense strands of the second half of a cDNA corresponding to nucleotides 598-1304 of the 57 kDa isoform of Slob . (Shahidullah et al., 2009)
Carried in construct	P{UAS-Slob.RNAi} (Shahidullah et al., 2009, Ma et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	neurophysiology defective (Ma et al., 2011) neurophysiology defective, with Scer\GAL4^Act.PU (Ma et al., 2011) neurophysiology defective, with Scer\GAL4^{cenB1A-Mai301} (Shahidullah et al., 2009)
Phenotype Manifest In
	adult pars intercerebralis, with Scer\GAL4^{cenB1A-Mai301} (Shahidullah et al., 2009)
Detailed Description
	Statement Reference The evoked excitatory junctional current (EJC) peak amplitude at the neuromuscular junction is approximately 30% higher than wild type in files expressing Slob[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act.PU]. The mutant flies show an increase in the frequency and amplitude of spontaneous miniature mEJCs compared to wild type. The EJC peak amplitude and the frequency and amplitude of mEJCs at the neuromuscular junction is increased compared to wild type in flies expressing Slob[dsRNA.Scer\UAS] under the control of Scer\GAL4[1407], but not in flies expressing Slob[dsRNA.Scer\UAS] under the control of Scer\GAL4[how-24B]. (Ma et al., 2011) Scer\GAL4[cenB1A-Mai301]-driven expression of Slob[dsRNA.Scer\UAS] results in altered neuronal excitability. Whole-cell patch recordings in pars intercerebralis (PI-1) neurons reveal an increase in outward current and hyperpolarizing shift in the conductance versus voltage (G-V) relationship compared to wild-type controls as a result of targeted Slob[dsRNA.Scer\UAS] expression. The shifts in the voltage at which half-maximal activation of the outward current occurs (V[[1/2]]) are modest, but highly significant. Compared to wild-types, the proportion of outward current blocked by the potassium channel blocker tetraethylammonium (TEA) is increased in flies expressing Scer\GAL4[cenB1A-Mai301]-driven Slob[dsRNA.Scer\UAS]. (Shahidullah et al., 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Slob^{dsRNA.Scer\UAS} is rescued by Scer\GAL4^{cenB1A-Mai301}/Slob^Scer\UAS.cSa (Shahidullah et al., 2009)
Comments	The outward current phenotype observed in mutants expressing Slob[dsRNA.Scer\UAS] under the control of Scer\GAL4[cenB1A-Mai301] is rescued by co-expressing Slob[Scer\UAS.cSa]. (Shahidullah et al., 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Expression of Slob[dsRNA.Scer\UAS] produces dsRNA which results in dsRNA interference (RNAi) of the Slob gene. (Shahidullah et al., 2009)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Slob^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Ma et al., 2011, J. Gen. Physiol. 137(2): 225--238 Slob, a Slowpoke channel-binding protein, modulates synaptic transmission. [FBrf0212896] Shahidullah et al., 2009, J. Neurosci. 29(42): 13328--13337 In vivo role of a potassium channel-binding protein in regulating neuronal excitability and behavior. [FBrf0209024]

Allele Dmel\SlobdsRNA.Scer\UAS

Allele Dmel\Slob^{dsRNA.Scer\UAS}