|Feature type||allele||Associated gene||Dmel\Slob|
|Mutagen||in vitro construct - regulatory fusion, in vitro construct - RNAi|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
The evoked excitatory junctional current (EJC) peak amplitude at the neuromuscular junction is approximately 30% higher than wild type in files expressing Slob[dsRNA.Scer\UAS] under the control of Scer\GAL4[Act.PU]. The mutant flies show an increase in the frequency and amplitude of spontaneous miniature mEJCs compared to wild type. The EJC peak amplitude and the frequency and amplitude of mEJCs at the neuromuscular junction is increased compared to wild type in flies expressing Slob[dsRNA.Scer\UAS] under the control of Scer\GAL4, but not in flies expressing Slob[dsRNA.Scer\UAS] under the control of Scer\GAL4[how-24B].
Scer\GAL4[cenB1A-Mai301]-driven expression of Slob[dsRNA.Scer\UAS] results in altered neuronal excitability. Whole-cell patch recordings in pars intercerebralis (PI-1) neurons reveal an increase in outward current and hyperpolarizing shift in the conductance versus voltage (G-V) relationship compared to wild-type controls as a result of targeted Slob[dsRNA.Scer\UAS] expression. The shifts in the voltage at which half-maximal activation of the outward current occurs (V[[1/2]]) are modest, but highly significant. Compared to wild-types, the proportion of outward current blocked by the potassium channel blocker tetraethylammonium (TEA) is increased in flies expressing Scer\GAL4[cenB1A-Mai301]-driven Slob[dsRNA.Scer\UAS].
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 2 )|