FB2025_02 , released April 17, 2025
Allele: Dmel\SlobRNAi.UAS
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General Information
Symbol
Dmel\SlobRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0243530
Feature type
allele
Associated gene
Dmel\Slob
Associated Insertion(s)
Carried in Construct
P{UAS-Slob.RNAi}
Also Known As
Slob-RNAi
Key Links
Transgenic product class
RNAi_reagent
(Shahidullah et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Shahidullah et al., 2009)
Mutagen
in vitro construct
(Shahidullah et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Slob.RNAi}
Cytology
Description

Two convergent arrays of UAS regulatory sequences drive the simultaneous transcription of both sense and antisense strands of the second half of a cDNA corresponding to nucleotides 598-1304 of the 57 kDa isoform of Slob .

(Shahidullah et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Slob
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The evoked excitatory junctional current (EJC) peak amplitude at the neuromuscular junction is approximately 30% higher than wild type in files expressing SlobdsRNA.Scer\UAS under the control of Scer\GAL4Act.PU. The mutant flies show an increase in the frequency and amplitude of spontaneous miniature mEJCs compared to wild type.

      The EJC peak amplitude and the frequency and amplitude of mEJCs at the neuromuscular junction is increased compared to wild type in flies expressing SlobdsRNA.Scer\UAS under the control of Scer\GAL41407, but not in flies expressing SlobdsRNA.Scer\UAS under the control of Scer\GAL4how-24B.

      (Ma et al., 2011)

      Scer\GAL4cenB1A-Mai301-driven expression of SlobdsRNA.Scer\UAS results in altered neuronal excitability. Whole-cell patch recordings in pars intercerebralis (PI-1) neurons reveal an increase in outward current and hyperpolarizing shift in the conductance versus voltage (G-V) relationship compared to wild-type controls as a result of targeted SlobdsRNA.Scer\UAS expression. The shifts in the voltage at which half-maximal activation of the outward current occurs (V[[1/2]]) are modest, but highly significant.

      Compared to wild-types, the proportion of outward current blocked by the potassium channel blocker tetraethylammonium (TEA) is increased in flies expressing Scer\GAL4cenB1A-Mai301-driven SlobdsRNA.Scer\UAS.

      (Shahidullah et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      SlobRNAi.UAS is rescued by Scer\GAL4CenB1A-Mai301/SlobUAS.cSa

      (Shahidullah et al., 2009)
      Comments

      The outward current phenotype observed in mutants expressing SlobdsRNA.Scer\UAS under the control of Scer\GAL4cenB1A-Mai301 is rescued by co-expressing SlobScer\UAS.cSa.

      (Shahidullah et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Expression of SlobdsRNA.Scer\UAS produces dsRNA which results in dsRNA interference (RNAi) of the Slob gene.

      (Shahidullah et al., 2009)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      SlobRNAi.UAS
      SlobdsRNA.Scer\UAS
      SlobdsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)