FlyBase Allele Report: Dmel\Slob[mut-IP1]

General Information

Symbol

Dmel\Slob^mut-IP1

Species

D. melanogaster

Name

FlyBase ID

FBal0243532

Feature type

allele

Associated gene

Dmel\Slob

Associated Insertion(s)

P{GawB}Slob^mut-IP1

Carried in Construct

Also Known As

mut^IP1

Key Links

Allele class

amorphic allele - molecular evidence

hypomorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Jepson et al., 2013)

hypomorphic allele - molecular evidence

(Ma et al., 2011)

Mutagen

Delta2-3 transposase

(Shahidullah et al., 2009)

Progenitor genotype

P{GawB}Slob^mut-K162

(Shahidullah et al., 2009)

Associated Insertion(s)

P{GawB}Slob^mut-IP1

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Ma et al., 2011)

abnormal neurophysiology | recessive

(Shahidullah et al., 2009)

abnormal neurophysiology | third instar larval stage

(Jepson et al., 2013)

abnormal stress response (with Df(2L)Exel6018)

(Shahidullah et al., 2009)

abnormal stress response (with Slob^mut-K162)

(Shahidullah et al., 2009)

abnormal stress response | recessive

(Shahidullah et al., 2009)

Phenotype Manifest In

adult pars intercerebralis

(Shahidullah et al., 2009)

Detailed Description

Statement

Reference

Action potentials can be evoked in wild-type larval motor neuron cell bodies by the injection of depolarising current. In Slob^mut-IP1 mutant larvae, more action potentials are evoked by the same depolarising stimulus at all stimulus levels examined.

Recordings of voltage-dependent currents under voltage clamp show that the fast inward currents in Slob^mut-IP1 mutants are similar to wild-type. Conversely, the voltage-dependent outward currents are smaller in the mutant.

(Jepson et al., 2013)

The evoked excitatory junctional current (EJC) peak amplitude at the neuromuscular junction is approximately 25% higher in mutant flies than in wild type. The mutant flies show a strong increase in the frequency and amplitude of spontaneous miniature mEJCs compared to wild type.

(Ma et al., 2011)

Slob^mut-IP1 mutants display altered neuronal excitability. Whole-cell patch recordings reveal that the outward current traces in pars intercerebralis (PI-1) neurons evoked by each depolarizing voltage step are larger in Slob^mut-IP1 mutants than in wild-type controls. Quantitative analysis reveals that the voltage dependence of activation, as measured by the conductance versus voltage (G-V) relationship, is shifted in the hyperpolarizing direction in the Slob^mut-IP1 mutant flies.

Compared to wild-types, the proportion of outward current blocked by the potassium channel blocker tetraethylammonium (TEA) is increased in Slob^mut-IP1 mutant flies.

In contrast to wild-type controls, single slo channels in PI-1 neurons appear to be open all the time, closing only occasionally and briefly in Slob^mut-IP1 mutants.

Action potential duration is shorter in PI-1 neurons in the brains of Slob^mut-IP1 mutant flies than in those in wild-type flies. This is due to an increase in the rate of repolarization, whereas the rise times of the action potentials are similar in the two genotypes.

Homozygous Slob^mut-IP1 or hemizygous Slob^mut-IP1/Df(2L)Exel6018 or trans-heterozygous Slob^mut-IP1/Slob^mut-K162 mutant flies survive starvation significantly longer than do wild-type or heterozygous control flies.

(Shahidullah et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Slob^mut-IP1 is rescued by Scer\GAL4^Act.PU/Slob^UAS.cSa

(Ma et al., 2011)

Slob^mut-IP1 is rescued by Scer\GAL4^insc-Mz1407/Slob^UAS.cSa

(Ma et al., 2011)

Slob^mut-IP1 is rescued by Scer\GAL4^Ilp2.PR/Slob^UAS.cSa

(Shahidullah et al., 2009)

Not rescued by

Slob^mut-IP1 is not rescued by Scer\GAL4^how-24B/Slob^UAS.cSa

(Ma et al., 2011)

Comments

Expression of Slob^Scer\UAS.cSa under the control of either Scer\GAL4^Act.PU or Scer\GAL4¹⁴⁰⁷ rescues the excitatory junctional current (EJC) peak amplitude of Slob^mut-IP1 neuromuscular junctions to wild-type levels, and also restores the frequency and amplitude of spontaneous miniature mEJCs to normal.

Expression of Slob^Scer\UAS.cSa under the control of Scer\GAL4^how-24B does not rescue the electrophysiological defects at the Slob^mut-IP1 neuromuscular junction.

(Ma et al., 2011)

The outward current phenotype observed in Slob^mut-IP1 mutants is rescued by Scer\GAL4^Ilp2.PR-driven expression of Slob^Scer\UAS.cSa.

(Shahidullah et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Slob^mut-IP1

mut^IP1

(Ma et al., 2011)

slob null

(Jepson et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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