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General Information
Symbol
Dmel\mars1
Species
D. melanogaster
Name
FlyBase ID
FBal0243582
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:

852bp deletion resulting from imprecise excision of P{EP}drkEP2477. 7 bases inserted at deletion site.

Inserted_sequence:

GATGAAA

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of P{EP}drkEP2477 generates a 0.84kb deletion that removes the mars initiation codon and part of the coding region.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous oocytes show defects in spindle morphology: microtubules often fail to be properly tapered at the spindle poles.

Eggs laid by mars1 and mars1/marsEP2477 flies show a greatly reduced ability to hatch. Sperm tails can be visualised in early-arrested embryos laid by mutant females, indicating that sterility is caused by arrest in embryogenesis after fertilization.

mars1 embryos, lacking maternal mars arrest during early embryogenesis after no more than five nuclear divisions. Approximately 82% of 15-45 minute old embryos laid by mars1 mothers exhibit at least two discrete DNA-containing regions, the first, localized to the embryonic cortext, resembling a polar body; and the second, surrounded by a bipolar spindle, is more central, indicating that the vast majority of mutant embryos pass though meiosis to form one or more mitotic figure.

The spindle structures in mars1 mutant embryos are very small and weak, albeit sill bipolar. Most exhibit at least one detached centrosome. Unaligned chromosomes are also found.

Embryos laid by mars1/marsEP2477 mothers resemble those from mars1 mothers, with less severe phenotypes.

mars1 heterozygous embryos appear wild-type and hatch normally.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Embryos laid by flies heterozygous for mars1 and Pp1-87B1 show a significantly reduced hatch ratio.

The lethality of embryos laid by mars1/marsEP2477 mothers is rescued by taccS863L.Ubi-p63E.T:Avic\GFP but not taccUbi-p63E.T:Avic\GFP expression.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of marsScer\UAS.P\T.cTa in the female germline, under the control of Scer\GAL4arm.PS restores embryo viability and fertility in mars1 and mars1/marsEP2477 mutants.

Ectopic expression of marsF839A.Scer\UAS.P\T under the control of Scer\GAL4arm.PS fails to rescue the embryonic lethality of embryos laid by mars1 or mars1/marsEP2477 mothers.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (2)