FB2025_01 , released February 20, 2025
Allele: Dmel\ncdHL.ncd.Venus
Open Close
General Information
Symbol
Dmel\ncdHL.ncd.Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0243598
Feature type
allele
Associated gene
Dmel\ncd
Associated Insertion(s)
Carried in Construct
P{ncd-ncd.HL.YFP}
Key Links
Transgenic product class
inframe_deletion
(Hallen et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Hallen et al., 2008)
Mutagen
in vitro construct
(Hallen et al., 2008)
Progenitor genotype
Carried in construct
P{ncd-ncd.HL.YFP}
Cytology
Description

Native ncd promoter regulates expression of a fragment encoding the ncd tail and coiled-coil stalk (M1-G347+N348) fused at the C terminus to Venus.

(Hallen et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
ncd
Encoded product / tool
ncd
Tagged with
Venus
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mature ncdHL.ncd.T:Avic\GFP-YFP.Venus;ncd1 oocytes show multi-polar or multiple small meiosis I spindles.

      The spindle and centrosomes appear normal throughout early cleavage divisions in ncdHL.ncd.T:Avic\GFP-YFP.Venus;ncd1 embryos, but abnormal mitotic spindles are frequently observed in embryogenesis, with abnormalities increasing in frequency in later divisions. Abnormalities include spindle spurs or branches resulting from mis-segregating chromosomes detaching from the spindle or moving off the metaphase plate, bent anaphase spindles, and poorly formed midzones in telophase. Free centrosomes are seen in some embryos, where a failure of spindle formation or centrosome loss from the spindle pole has occurred.

      ncdHL.ncd.T:Avic\GFP-YFP.Venus;ncd1 mitotic spindles are 1-2μm longer than control spindles in pro-metaphase and metaphase. This difference in length is increased to 3-4μum in anaphase and telophase.

      (Hallen et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      ncdHL.ncd.Venus fails to rescue ncd1

      (Hallen et al., 2008)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ncdHL.ncd.T:Avic\GFP-YFP.Venus
      ncdHL.ncd.Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (1)