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General Information
Symbol
Avic\GFPtrol-ZCL1973
Species
A. victoria
Name
FlyBase ID
FBal0243605
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
trol-GFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Protein-trap allele.

Insertion components
P{PTT-un}trolZCL1973
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

trol reporter expression becomes visible around cardioblasts at embryonic stage 14. By stage 16, expression also surrounds pericardial nephrocytes and the surface of the lymph gland. Shortly after larval hatching, expression is observed covering each individual hematopoietic progenitor of the lymph gland. Expression is particularly strong around cells of the posterior signalling center. By the second instar stage, trol expression forms a solid layer around the surface of the primary and secondary lobes of the lymph gland. Within the lobes, expression is seen strongly around clusters of cells. The trol reporter pattern outlines a system of chambers that each contain small clusters of hematopoietic progenitors or differentiated hemocytes. Basement membrane components collagen and laminin are co-exexpressed with trol in the lymph gland. The structures containing trol reporter are revealed as basement membrane by electron microscopy.

GFP-tagged trol protein encoded by Avic\GFPtrol-ZCL1973 is first detected in the basement membrane surrounding cardioblasts at embryonic stage 14. By embryonic stage 16, tagged trol protein is associated with the basement membranes of pericardial nephrocytes, and at the surface of the embryonic/larval lymph gland. In newly hatched larvae, tagged trol protein surrounds hematopoietic progenitors of the lymph gland; expression is pronounced in cells of the posterior signalling center of the lymph gland primary lobe. In second and third instar larvae, tagged trol protein is concentrated in the medullary zone and expression is decreased in the cortical zone of lymph glands.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

FlyBase Curator Comment: Insertion obtained from 'Flytrap; GFP Protein Trap Database'.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Avic\GFPtrol-ZCL1973
Name Synonyms
Secondary FlyBase IDs
    References (4)