Allele Dmel\CG17575^{dsRNA.Sym.Scer\UAS}

General Information
Symbol	Dmel\CG17575^{dsRNA.Sym.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0243743
Feature type	allele	Associated gene	Dmel\CG17575

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Ram and Wolfner, 2007) in vitro construct - RNAi (Ram and Wolfner, 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Two copies of Scer\UAS regulatory sequence encompass CG17575 coding sequences, so generating dsRNA. (Ram and Wolfner, 2007)
Carried in construct	P{symUAS-CG17575.RNAi} (Ram and Wolfner, 2007, Avila and Wolfner, 2009)
Cytology
Phenotypic Data
Phenotypic Class
	mating rhythm defective, with Scer\GAL4^αTub84B.PL (Ram and Wolfner, 2007)
Phenotype Manifest In

Detailed Description
	Statement Reference Mates of CG17575[dsRNA.Sym.Scer\UAS] Scer\GAL4[αTub84B.PL] males show no difference in conformational stage distribution when compared to mates of control males. In defensive sperm competition, assessed with a second mating on the third day after the initial mating, mates of CG17575[dsRNA.Sym.Scer\UAS] Scer\GAL4[αTub84B.PL] knockdown males sire a higher proportion of progeny than do control siblings. (Avila and Wolfner, 2009) Males depleted for CG17575, through expression of CG17575[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[αTub84B.PL], do not stimulate full egg laying in their mates. However, from days 2-10 after the start of mating, the number of eggs laid by the mates of CG17575[dsRNA.Sym.Scer\UAS]-Scer\GAL4[αTub84B.PL] males is reduced significantly compared to controls. Consistent with the reduced egg laying, females mated to CG17575[dsRNA.Sym.Scer\UAS] flies exhibit significantly lower fertility compared to controls. Females mated to CG17575[dsRNA.Sym.Scer\UAS]-Scer\GAL4[αTub84B.PL] males show low receptivity to re-mating 24 hours after the initial mating. At 4 days after the start of mating, mates of CG17575 knockdown males are significantly more receptive to mating than controls. Females mated to CG17575[dsRNA.Sym.Scer\UAS]-Scer\GAL4[αTub84B.PL] males do not differ significantly in the number of sperm in storage at 2 hours after the start of mating. At four days after the start of mating there is no significant difference in the number of sperm stored in the seminal receptacle of females mated to CG17575[dsRNA.Sym.Scer\UAS]-Scer\GAL4[αTub84B.PL] knockdown males compared to their controls. By ten days after the start of mating, females mated to CG17575[dsRNA.Sym.Scer\UAS]-Scer\GAL4[αTub84B.PL] knockdown males, have significantly more sperm stored in their seminal receptacle compared to controls. (Ram and Wolfner, 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	CG17575^{dsRNA.Sym.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Avila and Wolfner, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(37): 15796--15800 Acp36DE is required for uterine conformational changes in mated Drosophila females. [FBrf0208975] Ram and Wolfner, 2007, PLoS Genet. 3(12): e238 Sustained post-mating response in Drosophila melanogaster requires multiple seminal fluid proteins. [FBrf0204298]

Allele Dmel\CG17575dsRNA.Sym.Scer\UAS

Allele Dmel\CG17575^{dsRNA.Sym.Scer\UAS}