Allele Dmel\Gr33a^GAL4

General Information
Symbol	Dmel\Gr33a^GAL4	Species	D. melanogaster
Name		FlyBase ID	FBal0243781
Feature type	allele	Associated gene	Dmel\Gr33a

Allele class
Mutagen	recombination, SCEI endonuclease, FLPase, ends-out gene targeting
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-out gene targeting (Moon et al., 2009, Moon et al., 2009) FLPase (Moon et al., 2009) recombination (Moon et al., 2009) SCEI endonuclease (Moon et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{FRT(Gr33a.GAL4.Scer\SceI.RS)} (Moon et al., 2009)
Nature of the lesion	Statement Reference Ends-out homologous recombination at the Gr33a locus, using P{FRT(Gr33a.GAL4.Scer\SceI.RS)} as the donor template. (Moon et al., 2009) Ends-out homologous recombination at the Gr33a locus has resulted in the Scer\GAL4 coding region being inserted at the site of the normal translation initiation codon of the Gr33a locus (a mini-w marker has also been inserted in addition to the Scer\GAL4 coding region and a portion of the endogenous Gr33a coding region has been replaced by the inserted sequences). (Moon et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	courtship behavior defective \| male, with Gr33a¹ (Moon et al., 2009) neurophysiology defective (Moon et al., 2009, Moon et al., 2009) taste perception defective (Moon et al., 2009)
Phenotype Manifest In
	labellar taste bristle (Moon et al., 2009, Moon et al., 2009)
Detailed Description
	Statement Reference Mutant flies show normal L-canavanine avoidance in a two-way choice test. (Lee et al., 2012) Mutant labellar taste bristles show a normal electrophysiological response to 50mM sucrose. Mutant flies show a normal behavioural response to sucrose in a two-way choice test (preferring 5mM sucrose to 1mM sucrose). Mutant labellar taste bristles show an impaired electrophysiological response to a number of bitter tasting compounds (caffeine, quinine, denatonium, berberine, lobeline, papaverine and strychnine). (Moon et al., 2009) Mutant labellar taste bristles show an impaired electrophysiological response to a number of bitter tasting compounds (caffeine, quinine, denatonium, berberine, lobeline, papaverine and strychnine). Mutant flies show reduced avoidance to a number of bitter compounds (caffeine, quinine, denatonium, berberine, lobeline and papaverine) in a two-way choice assay compared to control flies. Gr33a[1]/Gr33a[GAL4] males show increased courtship towards passive, decapitated wild-type males compared to the behaviour of control males in this assay. However, courtship of the mutant males to decapitated or normal females is not significantly different from that of wild type. Wild-type males show a normal average time to mating when mated with Gr33a[1]/Gr33a[GAL4] females. (Moon et al., 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Gr33a¹/Gr33a^GAL4 is rescued by Scer\GAL4^Gr33a-GAL4/Gr33a^Scer\UAS.cMa (Moon et al., 2009, Moon et al., 2009)
Comments
Stocks ( 1 )
Bloomington	31425 w^*; Gr33a^GAL4
Notes on Origin
Discoverer
	Associated with: Scer\GAL4[Gr33a-GAL4]. (Moon et al., 2009)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Gr33a^GAL4 (Lee et al., 2012, Moon et al., 2009, Moon et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Lee et al., 2012, J. Neurosci. 32(4): 1429--1435 Gustatory receptors required for avoiding the insecticide L-canavanine. [FBrf0217317] Moon et al., 2009, Curr. Biol. 19(19): 1623--1627 A Drosophila gustatory receptor essential for aversive taste and inhibiting male-to-male courtship. [FBrf0209074]
Supplementary material	Moon et al., 2009, Curr. Biol. 19(19): Supplemental Data. [FBrf0210973]

Allele Dmel\Gr33aGAL4

Allele Dmel\Gr33a^GAL4