Open Close
General Information
Symbol
Scer\GAL4Gr33a-GAL4
Species
S. cerevisiae
Name
FlyBase ID
FBal0243785
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Gr33a-Gal4, Gr33aGAL4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Sequence encoding a GAL4 driver has been inserted at the site of the normal translation initiation codon of the Gr33a locus (a mini-w marker has also been inserted in addition to the GAL4 coding region and a portion of the endogenous Gr33a coding region has been replaced by the inserted sequences).

Insertion components
TI{GAL4}Gr33aGAL4
Product class / Tool use(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4Gr33a-GAL4 drives expression in bitter-sensing neurons in the larval dorsal pharyngeal organ.

Drives expression in bitter-activated gustatory receptor neurons in the adult labellum and in the tarsal segments of legs.

Scer\GAL4Gr33a-GAL4 drives expression in S-a and S-b bitter-activated gustatory receptor neurons in the adult labellum.

Scer\GAL4Gr33a-GAL4 drives expression in L-canavanine-insensitive S2- and I-type sensilla.

Scer\GAL4Gr33a-GAL4 drives expression in all I-type and S-type sensilla, which represent a total of 22 gustatory receptor neurons in each labellum.

Scer\GAL4Gr33a-GAL4 drives expression in two of the three gustatory receptor neurons innervating the dorsolateral terminal organ. These neurons coexpress ppk23, but not Scer\GAL4GMR58F10

Scer\GAL4Gr33a-GAL4 drives expression in about 20 gustatory receptor neurons (GRNs) in the adult labellum; these GRNs coexpress Gr66a. Scer\GAL4Gr33a-GAL4 also drives expression in multiple gustory receptor neurons in the tarsal segments of the legs; these include 7 in each prothoracic leg, 4 in each mesothoracic leg, and 4 in each methoracic leg. Several of the prothoracic GRNs coexpress Scer\GAL4Gr32a.3.776.

 
Marker for
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Ctet\tetXTNT-LC.UAS or Hsap\KCNJ2UAS.EGFP) of Scer\GAL4Gr33a-GAL4 cells in adults results in a significant increase in proboscis extensions in response to higher concentrations of hexanoic acid, with no effect on the response to sucrose, compared to controls.

Inhibition (using Hsap\KCNJ2UAS.EGFP) of the combined set of Scer\GAL4Gr33a-GAL4 and Scer\GAL4Gr64f.9.7 cells in adult females does not affect preference for oviposition on acid-containing medium, compared to controls.

Males in which neurons expressing Gr33aGAL4 have been silenced show no difference in courtship intensity as compared with wild type or controls, but lose preference for younger females, instead showing no significant preference for either younger or older females.

Inhibition (using Ctet\TeTxLCTNT.UAS) of Scer\GAL4Gr33a-GAL4 cells does not affect the preference of larvae for larval-treated substrate, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (22)