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General Information
Symbol
Dmel\Pi3K59FΔm22
Species
D. melanogaster
Name
FlyBase ID
FBal0243862
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
vps34Δm22
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion resulting in a deletion extending 3349 bp downstream of the insertion site. The entire Pi3K59F coding region is removed.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Pi3K59FΔm22 somatic clones in the follicular epithelium exhibit dysplasia-like defects, namely a high frequency of epithelium multilayering and mild cell polarity defects (i.e. abnormal expression of the adherent junction components Arm and E-cad, and occasional overlap between the apical marker aPKC and the basolateral marker Dlg), with cells occasionally invading between germline cells, as compared to controls.

Homozygous adult mushroom body gamma neuron clones show an axon pruning defect.

Expression of Pi3K59FΔm22 using Scer\GAL4Act5C.PP results in basal autophagy defects in larval fat body cell somatic clones.

Pi3K59FΔm22 mutants display basal autophagy defects in larval fat body cells.

Females carrying homozygous germline clones have a significant increase in the number of stage 14 egg chambers that have persisting TUNEL-negative nurse cell nuclei compared to wild-type egg chambers at the same stage, in which nurse cell nuclei are rarely detected (and those that are detected are all TUNEL positive). The persisting nurse cell nuclei show condensed nuclear staining.

Starvation-induced expansion of the lysosomal system is blocked in Pi3K59FΔm22 mutant fat-body cells. Autophagosome and autolysosome formation is disrupted in these animals. Fat-body cell size is normal.

Pi3K59FΔm22 mutant fat-body and garland cells show disruptions in endocytosis.

In the eye imaginal disc, Pi3K59FΔm22 mutant cells proliferate at a rate similar to that of twin spot control cells. No accumulation of autophagosomes is observed in mutant eye disc cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The frequent follicular epithelium multi-layering and the mis-localization of cell polarity markers presented by Pi3K59FΔm22 homozygous follicle cell clones is strongly suppressed by the clonal expression of Lkb1HMS01351 under the control of Scer\GAL4tub.PU.

Both the frequent follicular epithelium multi-layering and the occasional invasion of germline cells by follicle cells exhibited by Pi3K59FΔm22 homozygous follicle cell clones is strongly suppressed by the clonal expression of bskDN.Scer\UAS.cUa under the control of Scer\GAL4tub.PU.

The lack of F-actin rich radial protrusions seen in primary hemocytes derived from mtmΔ77/mtmz2-4747 larvae is not rescued if the larvae also carry Pi3K59FΔm22/Pi3K59FΔm22.

Expression of Tsc1Scer\UAS.cTa and gigScer\UAS.cTa under the control of Scer\GAL4Act5C.PP results in a significant reduction in fat-body cell size in a Pi3K59FΔm22 background, and the formation of autolysosomes is disrupted.

Pi3K59FΔm22 and Vps25A3 double mutant fat-body cells show disruptions in endocytosis.

Vps25A3 and Pi3K59FΔm22 double mutant clones generated in the fat-body or eye-disc show accumulation of autophagosomes under fed conditions, compared to none observed in Pi3K59FΔm22 single mutant clones.

Vps28k16503 and Pi3K59FΔm22 double mutant clones generated in the fat-body show accumulation of autophagosomes under fed conditions, compared to none observed in Pi3K59FΔm22 single mutant clones.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

The follicular epithelium multi-layering exhibited by Pi3K59FΔm22 homozygous somatic clones is almost fully rescued by the expression of Pi3K59FScer\UAS.cJa under the control of Scer\GAL4tub.PU.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (10)