FB2025_01 , released February 20, 2025
Allele: Dmel\Rca1UAS.Tag:HA.Tag:FLAG
Open Close
General Information
Symbol
Dmel\Rca1UAS.Tag:HA.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0243881
Feature type
allele
Associated gene
Dmel\Rca1
Associated Insertion(s)
Carried in Construct
P{UAS-Rca1.HA.FLAG}
Also Known As
UAS-rca1
Key Links
Transgenic product class
wild_type
(Zielke et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Zielke et al., 2006)
Mutagen
in vitro construct
(Zielke et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Rca1.HA.FLAG}
Cytology
Description

UAS regulatory sequences drive expression of full-length Rca1, tagged at the C-terminus with Tag:HA and Tag:FLAG.

(Zielke et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Rca1
Tagged with
Tag:FLAG
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Over-expression of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU in the eye results in a rough eye phenotype. Ectopic cell division is observed in the eye disc, posterior to the morphogenetic furrow.

      Over-expression of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG in the wing under the control of Scer\GAL4Act5C.PI increases the rate at which cells progress through the cell cycle from G1 to S-phase. Cell size is normal.

      (Zielke et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Over-expression of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG and dapScer\UAS.cLa under the control of Scer\GAL4GMR.PU does not alter the ability of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG to induce ectopic cell division in the eye disc.

      (Zielke et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4prd.RG1/Rca1UAS.Tag:HA.Tag:FLAG rescues Rca12

      (Zielke et al., 2006)

      Scer\GAL4Tub.PU/Rca1UAS.Tag:HA.Tag:FLAG rescues Rca12

      (Zielke et al., 2006)
      Comments

      Expression of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4prd.RG1 in a Rca12 mutant background restores embryonic cell numbers to wild-type levels in Scer\GAL4prd.RG1-expressing segments.

      Expression of Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4tub in Rca12 mutant wing disc clones suppresses the proliferation disadvantage observed in Rca12 mutant tissue, and clones are similar in size to wild-type. No endoreplication is observed in these cells and nuclear size is comparable to wild-type cells.

      (Zielke et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG
      Rca1UAS.Tag:HA.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (3)