FlyBase Allele Report: Dmel\Mes2[UAS.cZa]

General Information

Symbol

Dmel\Mes2^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Zimmermann

FlyBase ID

FBal0243915

Feature type

allele

Associated gene

Dmel\Mes2

Associated Insertion(s)

Carried in Construct

P{UAS-Mes2.Z}

Key Links

Transgenic product class

cDNA

(Zimmermann et al., 2006)

wild_type

(Zimmermann et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zimmermann et al., 2006)

wild_type

(Zimmermann et al., 2006)

Mutagen

in vitro construct

(Zimmermann et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Mes2.Z}

Cytology

Description

UASt regulatory sequences drive expression of the entire region of the Mes2 EST SD09884.

(Zimmermann et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mes2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^da.G32

(Zimmermann et al., 2006)

lethal, with Scer\GAL4^nanos.PG

(Zimmermann et al., 2006)

lethal, with Scer\GAL4^prd.RG1

(Zimmermann et al., 2006)

lethal, with Scer\GAL4^repo.PU

(Zimmermann et al., 2006)

lethal, with Scer\GAL4^Tub.PU

(Zimmermann et al., 2006)

lethal, with Scer\GAL4^Ubi.PU

(Zimmermann et al., 2006)

lethal - all die before end of first instar larval stage, with Scer\GAL4^how-24B, Scer\GAL4^twi.PB

(Zimmermann et al., 2006)

lethal - all die before end of first instar larval stage, with Scer\GAL4^Mef2.PR, Scer\GAL4^twi.PB

(Zimmermann et al., 2006)

lethal - all die before end of first instar larval stage, with Scer\GAL4^twi.2PE

(Zimmermann et al., 2006)

lethal - all die before end of larval stage, with Scer\GAL4^71B

(Zimmermann et al., 2006)

lethal - all die before end of pupal stage, with Scer\GAL4^Bx-MS1096

(Zimmermann et al., 2006)

lethal - all die before end of second instar larval stage, with Scer\GAL4^gcm.PU

(Zimmermann et al., 2006)

lethal - all die during embryonic stage, with Scer\GAL4^69B

(Zimmermann et al., 2006)

some die during embryonic stage, with Scer\GAL4^69B

(Zimmermann et al., 2006)

some die during first instar larval stage, with Scer\GAL4^how-24B, Scer\GAL4^twi.PB

(Zimmermann et al., 2006)

some die during first instar larval stage, with Scer\GAL4^Mef2.PR, Scer\GAL4^twi.PB

(Zimmermann et al., 2006)

some die during first instar larval stage, with Scer\GAL4^twi.2PE

(Zimmermann et al., 2006)

some die during larval stage, with Scer\GAL4^71B

(Zimmermann et al., 2006)

some die during pupal stage, with Scer\GAL4^Bx-MS1096

(Zimmermann et al., 2006)

some die during second instar larval stage, with Scer\GAL4^gcm.PU

(Zimmermann et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^twi.2PE results in embryonic or first instar larval lethality at 25[o]C. Animals raised at 18[o]C are viable. The morphology of the mesoderm is not disrupted.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^twi.PB and Scer\GAL4^how-24B results in embryonic or first instar larval lethality at both 18[o]C and 25[o]C. The morphology of the mesoderm is not disrupted.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^twi.PB and Scer\GAL4^Mef2.PR results in first instar larval lethality at 25[o]C. Animals raised at 18[o]C are viable. The morphology of the mesoderm is not disrupted.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^gcm.PU results in second instar larval lethality at 25[o]C. Animals raised at 18[o]C are viable. The morphology of the glia are not disrupted.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^repo.PU results in lethality. The morphology of the glia are not disrupted.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^tub results in embryonic lethality in animals raised at 18[o]C or 25[o]C.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^Ubi results in lethality in animals raised at 18[o]C or 25[o]C. Gross developmental abnormalities are observed in these animals, including a failure of dorsal closure.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^nos.PG results in lethality. Gross developmental abnormalities are observed in these animals, including a failure of dorsal closure.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^da.G32 results in embryonic lethality. Gross developmental abnormalities are observed in these animals, including a failure of dorsal closure.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^prd.RG1 results in lethality.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^69B results in embryonic lethality. These embryos are morphologically wild-type up to stage 11, but by stage 13 the organisation of the epidermis is severely disrupted, especially along the leading edge that contacts cells of the amnioserosa. The cells of the leading edge do not display a normal elongated morphology and the integrity of the epidermis is disrupted, with "tears" in the epidermal sheath evident.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^Bx-MS1096 results in pupal lethality. Adult escapers have severely deformed wing and haltere tissues that form a necrotic mass at 25[o]C, and those raised at 18[o]C also show disrupted wing tissue development.

Expression of Mes2^Scer\UAS.cZa under the control of Scer\GAL4^71B results in larval/pupal lethality at both 18[o]C and 25[o]C.

(Zimmermann et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mes2^Scer\UAS.cZa

Mes2^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Zimmermann

Secondary FlyBase IDs

References (1)

Report Sections

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