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General Information
Symbol
Dmel\NUAS.Tag:V5,Tag:polyHis
Species
D. melanogaster
Name
FlyBase ID
FBal0243919
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-N-V5
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of full-length N which is tagged with Tag:V5 and Tag:polyHis tags after the sequence QQLGG in the N intracellular domain.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The clonal expression of NUAS.Tag:V5,Tag:polyHis under the control of Scer\GAL4esg-NP5130 leads to a strong reduction in the number of progenitor cells in renal tubules, as compared to controls.

The expression of NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407 or Scer\GAL4pnt-14-94 leads to severe increases in the numbers of total or type II neuroblasts, respectively, per third instar larval brain lobe, as compared to controls.

Larval brains expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407 exhibit ectopic formation and increased numbers of type II neuroblasts, as compared to controls.

Expression of NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of either Scer\GAL4insc-Mz1407 or Scer\GAL4pnt-14-94 results in an increase in the number of type II neuroblasts in the central brain compared to wild type.

Expression of NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4GMR9D11 results in an increase in the number of brain neuroblasts compared to wild type.

Larval brains overexpressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407 contain supernumerary neuroblasts.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of ctHMS00924 and NUAS.Tag:V5,Tag:polyHis under the control of Scer\GAL4esg-NP5130 leads to the abnormal differentiation of renal tubule progenitors into enterocyte-like cells (i.e. Pdm1-positive cells).

The increased number of neuroblasts in third instar larval brains resulting from the expression of NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407 is fully suppressed by the co-expression of Cdk9D199N.Scer\UAS.SR alone, but only partially suppressed by the combined co-expression of Cdk9D199N.Scer\UAS.SR and E(spl)mγ-HLHScer\UAS.cLb.

Co-expression of bansponge.Scer\UAS.T:Disc\RFP (but not of both bansponge.Scer\UAS.T:Disc\RFP and numbHMS01459, or both bansponge.Scer\UAS.T:Disc\RFP and MycScer\UAS.cZa, or both bansponge.Scer\UAS.T:Disc\RFP and CG8184HMS01605 or a combination of expression of bansponge.Scer\UAS.T:Disc\RFP and the presence of numb15/+) leads to partial suppression of the ectopic neuroblast formation and increased neuroblast numbers seen in larval brains expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

The presence of pros17/+ or bratk06028/+, or the co-expression of CycEScer\UAS.cLa, does not change the number of neuroblasts per brain lobe seen in larval brains expressing both bansponge.Scer\UAS.T:Disc\RFP and NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Co-expression of numbHMS01459 or presence of numb15/+ fails to suppress the increased neuroblast number seen in larval brains expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Co-expression of banD.Scer\UAS.T:Avic\GFP-EGFP leads to enhancement of the ectopic neuroblast formation and increased neuroblast number phenotypes seen in larval brains expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Co-expression of either Drp1K38A.Scer\UAS.T:Ivir\HA1, srlHMS00857, ND75NIG.2286R, rictorJF01086 or Akt1HMS00007 suppresses the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Co-expression of either CG17856GD9481 or trcK122A+T453A.Scer\UAS does not suppress the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Expression of NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4Mhc.PW suppresses the abnormal wing posture of Pink1B9 flies.

Expression of NGD14477 under the control of Scer\GAL4Mhc.PW does not suppress the abnormal wing posture of Pink1B9 flies.

Co-expression of NScer\UAS.T:SV5\V5,T:Zzzz\His6 suppresses the aggregation of mitochondria seen in the indirect flight muscles of animals expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Simultaneous co-expression of both Pink1dsRNA.Scer\UAS and Su(H)HM05110 suppresses the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Simultaneous co-expression of both Pink1dsRNA.Scer\UAS and dmKK103869 suppresses the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4insc-Mz1407.

Co-expression of either Pink1Scer\UAS.cYa or rictorScer\UAS.cHa enhances the increase in the number of type II neuroblasts which is seen in the central brain of animals expressing NScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4pnt-14-94.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
NScer\UAS.T:SV5\V5,T:Zzzz\His6
NUAS.Tag:V5,Tag:polyHis
Name Synonyms
Secondary FlyBase IDs
    References (7)