FlyBase Allele Report: Dmel\Vap33[UAS.cRa]

General Information

Symbol

Dmel\Vap33^UAS.cRa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ratnaparkhi

FlyBase ID

FBal0243926

Feature type

allele

Associated gene

Dmel\Vap33

Associated Insertion(s)

Carried in Construct

P{UAS-Vap-33-1.R}

Also Known As

UAS-VAP^wt

Key Links

Transgenic product class

wild_type

(Ratnaparkhi et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ratnaparkhi et al., 2008)

Mutagen

in vitro construct

(Ratnaparkhi et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Vap-33-1.R}

Cytology

Description

UAS regulatory sequences drive expression of wild-type Vap33.

(Ratnaparkhi et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Vap33

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^Δ166, Vap33^P58S.cRa.UAS

(Tendulkar et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PLu

(Ratnaparkhi et al., 2008)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Ratnaparkhi et al., 2008)

lethal, with Scer\GAL4^Act.PU

(Ratnaparkhi et al., 2008)

lethal, with Scer\GAL4^Tub.PU

(Ratnaparkhi et al., 2008)

lethal - all die before end of larval stage, with Scer\GAL4^how-24B

(Ratnaparkhi et al., 2008)

lethal - all die before end of P-stage, with Scer\GAL4^G14

(Ratnaparkhi et al., 2008)

some die during larval stage, with Scer\GAL4^how-24B

(Ratnaparkhi et al., 2008)

some die during pharate adult stage, with Scer\GAL4^G14

(Ratnaparkhi et al., 2008)

viable, with Scer\GAL4^elav.PLu

(Ratnaparkhi et al., 2008)

viable, with Scer\GAL4^repo

(Ratnaparkhi et al., 2008)

visible, with Scer\GAL4^sca.PU

(Ratnaparkhi et al., 2008)

Phenotype Manifest In

macrochaeta, with Scer\GAL4^sca.PU

(Ratnaparkhi et al., 2008)

NMJ bouton, with Scer\GAL4^elav.PLu

(Ratnaparkhi et al., 2008)

NMJ bouton, with Scer\GAL4^RapGAP1-OK6

(Ratnaparkhi et al., 2008)

terminal bouton, with Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

Detailed Description

Statement

Reference

Expression of Vap-33A^Scer\UAS.cRa under the control of Scer\GAL4^sca.PU results in temperature-sensitive bristle loss. Higher levels of bristle loss are seen at 28[o] than at 25[o]C.

Expression of Vap-33A^Scer\UAS.cRa under the control of Scer\GAL4^elav-C155 results in reduced bouton size compared to controls.

(Deivasigamani et al., 2014)

Expression of Vap-33-1^Scer\UAS.cRa under the control of Scer\GAL4^how-24B results in lethality at the first or second larval instar stage.

Animals expressing Vap-33-1^Scer\UAS.cRa under the control of Scer\GAL4^G14 are unable to eclose from the pupal case and die as pharate adults.

Expression of Vap-33-1^Scer\UAS.cRa under the control of either Scer\GAL4^elav.PLu or Scer\GAL4^OK6 results in a reduction in bouton diameter at the neuromuscular junction in third instar larvae. These boutons contain fewer neurotransmitter vesicles than control boutons in animals expressing Vap-33-1^Scer\UAS.cRa under the control of Scer\GAL4^elav.PLu.

The dorsal macrochaetae of the adult thorax either fail to develop or are dramatically reduced in number in animals expressing Vap-33-1^Scer\UAS.cRa under the control of Scer\GAL4^sca.PU. Co-expression of Vap-33-1^{P58S.cRa.Scer\UAS} suppresses the thoracic bristle defects seen in animals expressing Vap-33-1^Scer\UAS.cRa under the control of Scer\GAL4^sca.PU.

(Ratnaparkhi et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, Vap33^UAS.cRa has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^elav-C155/Tsc1^JF01262

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^elav-C155/Thor^LL.UAS

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has abnormal neuroanatomy phenotype, suppressible by Atg1^UAS.cCa, Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has abnormal neuroanatomy phenotype, suppressible by S6k^STDETE.UAS, Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

Scer\GAL4^sca.PU, Vap33^UAS.cRa has visible phenotype, suppressible by Scer\GAL4^sca.PU/Sod1^RNAi.UAS.cHa

(Deivasigamani et al., 2014)

Scer\GAL4^sca.PU, Vap33^UAS.cRa has visible phenotype, suppressible by CG7564^NIG.7564R, Scer\GAL4^sca.PU

(Deivasigamani et al., 2014)

Scer\GAL4^sca.PU, Vap33^UAS.cRa has visible phenotype, suppressible by Scer\GAL4^sca.PU/TBPH^NIG.10327R

(Deivasigamani et al., 2014)

Scer\GAL4^sca.PU, Vap33^UAS.cRa has visible phenotype, suppressible by Scer\GAL4^sca.PU/tkv^1ΔGSK.UAS

(Ratnaparkhi et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, Vap33^UAS.cRa has abnormal neuroanatomy phenotype, non-suppressible by gig^UAS.cTa/Scer\GAL4^elav-C155/Tsc1^UAS.cTa

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^elav-C155/mTor^TED.UAS

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has abnormal neuroanatomy phenotype, non-suppressible by S6k^KQ.UAS, Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^sca.PU, Vap33^UAS.cRa has macrochaeta phenotype, suppressible by Scer\GAL4^sca.PU/Sod1^RNAi.UAS.cHa

(Deivasigamani et al., 2014)

Scer\GAL4^sca.PU, Vap33^UAS.cRa has macrochaeta phenotype, suppressible by CG7564^NIG.7564R, Scer\GAL4^sca.PU

(Deivasigamani et al., 2014)

Scer\GAL4^sca.PU, Vap33^UAS.cRa has macrochaeta phenotype, suppressible by Scer\GAL4^sca.PU/TBPH^NIG.10327R

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has terminal bouton phenotype, suppressible by Scer\GAL4^elav-C155/Tsc1^JF01262

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has terminal bouton phenotype, suppressible by Scer\GAL4^elav-C155/Thor^LL.UAS

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has terminal bouton phenotype, suppressible by Atg1^UAS.cCa, Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has terminal bouton phenotype, suppressible by S6k^STDETE.UAS, Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

Scer\GAL4^sca.PU, Vap33^UAS.cRa has macrochaeta phenotype, suppressible by Scer\GAL4^sca.PU/tkv^1ΔGSK.UAS

(Ratnaparkhi et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, Vap33^UAS.cRa has terminal bouton phenotype, non-suppressible by gig^UAS.cTa/Scer\GAL4^elav-C155/Tsc1^UAS.cTa

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has terminal bouton phenotype, non-suppressible by Scer\GAL4^elav-C155/mTor^TED.UAS

(Deivasigamani et al., 2014)

Scer\GAL4^elav-C155, Vap33^UAS.cRa has terminal bouton phenotype, non-suppressible by S6k^KQ.UAS, Scer\GAL4^elav-C155

(Deivasigamani et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Sod^{dsRNA.Scer\UAS.cHa} suppresses the loss of bristle phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^sca.PU.

Expression of CG7564^NIG.7564R suppresses the loss of bristle phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^sca.PU.

Expression of TBPH^NIG.10327R suppresses the loss of bristle phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^sca.PU.

Co-expression of Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa does not suppress the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

Expression of Tsc1^JF01262 suppresses the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

Expression of Tor^TED.Scer\UAS does not suppress the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

Expression of Thor^LL.Scer\UAS suppresses the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

Expression of Atg1^Scer\UAS.cCa suppresses the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

Expression of S6k^KQ.Scer\UAS does not suppress the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

Expression of S6k^{STDETE.Scer\UAS} suppresses the small bouton phenotype seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^elav-C155.

(Deivasigamani et al., 2014)

Co-expression of tkv^{1ΔGSK.Scer\UAS} partially suppresses the loss of thoracic bristles seen in adults expressing Vap-33-1^Scer\UAS.cRa under the control of Scer\GAL4^sca.PU.

(Ratnaparkhi et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Vap33^UAS.cRa is rescued by Scer\GAL4^sca.PU/Vap33^NIG.5014R

(Deivasigamani et al., 2014)

Partially rescues

Vap33^UAS.cRa/Scer\GAL4^elav.PLu partially rescues Vap33^Δ166

(Ratnaparkhi et al., 2008)

Vap33^UAS.cRa/Scer\GAL4^elav.PLu partially rescues Vap33^Δ20

(Ratnaparkhi et al., 2008)

Comments

Expression of Vap-33A^NIG.5014R rescues the bristle loss seen when Vap-33A^Scer\UAS.cRa is expressed under the control of Scer\GAL4^sca.PU.

(Deivasigamani et al., 2014)

Expression of Vap-33-1^Scer\UAS.cRa under the control of Scer\GAL4^elav.PLu rescues the enlarged bouton phenotype seen at the neuromuscular junction of Vap-33-1^Δ166 third instar larvae.

The lethality of hemizygous Vap-33-1^Δ166 and Vap-33-1^Δ20 males is partially rescued by expression of Vap-33-1^Scer\UAS.cRa under the control of Scer\GAL4^elav.PLu.

(Ratnaparkhi et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Vap-33-1^Scer\UAS.cRa

Vap-33A^Scer\UAS.cRa

Vap33^Scer\UAS.cRa

Vap33^UAS.cRa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ratnaparkhi

Secondary FlyBase IDs

References (4)

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