Allele Dmel\Vap-33-1^{P58S.cRa.Scer\UAS}

General Information
Symbol	Dmel\Vap-33-1^{P58S.cRa.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0243927
Feature type	allele	Associated gene	Dmel\Vap-33-1

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Ratnaparkhi et al., 2008) in vitro construct - regulatory fusion (Ratnaparkhi et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a mutant form of Vap-33-1 which contains the amino acid replacement P58S. (Ratnaparkhi et al., 2008)
Carried in construct	P{UAS-Vap-33-1.P58S.R} (Ratnaparkhi et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| heat sensitive, with Scer\GAL4^how-24B (Ratnaparkhi et al., 2008) lethal \| heat sensitive, with Scer\GAL4^tub.PU (Ratnaparkhi et al., 2008) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^elav.PLu (Ratnaparkhi et al., 2008) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^Rapgap1-OK6 (Ratnaparkhi et al., 2008) viable, with Scer\GAL4^Act.PU (Ratnaparkhi et al., 2008) viable, with Scer\GAL4^elav.PLu (Ratnaparkhi et al., 2008) viable, with Scer\GAL4^repo (Ratnaparkhi et al., 2008)
Phenotype Manifest In
	NMJ bouton, with Scer\GAL4^elav.PLu (Ratnaparkhi et al., 2008) NMJ bouton, with Scer\GAL4^Rapgap1-OK6 (Ratnaparkhi et al., 2008)
Detailed Description
	Statement Reference Expression of Vap-33-1[P58S.cRa.Scer\UAS] under the control of Scer\GAL4[how-24B] or Scer\GAL4[tub] does not cause lethality at 25[o]C, but does result in lethality at 30[o]C. Expression of Vap-33-1[P58S.cRa.Scer\UAS] under the control of either Scer\GAL4[elav.PLu] or Scer\GAL4[OK6] results in an increase in bouton diameter at the neuromuscular junction in third instar larvae. Animals expressing Vap-33-1[P58S.cRa.Scer\UAS] under the control of Scer\GAL4[elav.PLu] show a significant increase in "floating T-bars" (electron dense structures attached to neurotransmitter vesicles floating within the cytoplasmic space) compared to controls. Expression of Vap-33-1[P58S.cRa.Scer\UAS] under the control of Scer\GAL4[sca.PU] has no effect on the development of the adult thoracic bristles. Co-expression of Vap-33-1[P58S.cRa.Scer\UAS] suppresses the thoracic bristle defects seen in animals expressing Vap-33-1[Scer\UAS.cRa] under the control of Scer\GAL4[sca.PU]. (Ratnaparkhi et al., 2008)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Partially rescues	Vap-33-1^{P58S.cRa.Scer\UAS}/Scer\GAL4^elav.PLu partially rescues Vap-33-1^Δ20 (Ratnaparkhi et al., 2008) Vap-33-1^{P58S.cRa.Scer\UAS}/Scer\GAL4^elav.PLu partially rescues Vap-33-1^Δ166 (Ratnaparkhi et al., 2008)
Comments	Expression of Vap-33-1[P58S.cRa.Scer\UAS] under the control of Scer\GAL4[elav.PLu] does not rescue the enlarged bouton phenotype seen at the neuromuscular junction of Vap-33-1[Δ166] third instar larvae. The lethality of hemizygous Vap-33-1[Δ166] and Vap-33-1[Δ20] males is partially rescued by expression of Vap-33-1[P58S.cRa.Scer\UAS] under the control of Scer\GAL4[elav.PLu]. (Ratnaparkhi et al., 2008)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Vap-33-1^{P58S.cRa.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Ratnaparkhi et al., 2008, PLoS ONE 3(6): e2334 A Drosophila model of ALS: human ALS-associated mutation in VAP33A suggests a dominant negative mechanism. [FBrf0210268]

Allele Dmel\Vap-33-1P58S.cRa.Scer\UAS

Allele Dmel\Vap-33-1^{P58S.cRa.Scer\UAS}