Allele Dmel\Acp36DE^{dsRNA.Sym.Scer\UAS}

General Information
Symbol	Dmel\Acp36DE^{dsRNA.Sym.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0243951
Feature type	allele	Associated gene	Dmel\Acp36DE

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Avila and Wolfner, 2009) in vitro construct - RNAi (Avila and Wolfner, 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Two copies of Scer\UAS regulatory sequence encompass 93bp of Acp36DE 3'UTR sequences, so generating dsRNA. (Avila and Wolfner, 2009)
Carried in construct	P{symUAS-Acp36DE.RNAi} (Avila and Wolfner, 2009)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	female reproductive system, with Scer\GAL4^αTub84B.PL (Avila and Wolfner, 2009) uterus, with Scer\GAL4^αTub84B.PL (Avila and Wolfner, 2009)
Detailed Description
	Statement Reference Mates of Acp36DE[dsRNA.Sym.Scer\UAS] Scer\GAL4[αTub84B.PL] males fail to reach the final stage of the uterine conformational changes; the majority of Acp36DE[dsRNA.Sym.Scer\UAS] knockdown mates appear to have stalled in the 'middle' stages. After stage 5, the reproductive tracts of mates of Acp36DE[dsRNA.Sym.Scer\UAS] knockdown males do no proceed efficiently, or completely, through the subsequent stages. With the conformational stages blocked at the hourglass stage, the sperm do not condense into a single mass. Instead, the sperm mass remains below the hourglass constriction, becoming more dispersed than normal in the posterior uterus. Sperm storage in Acp36DE[dsRNA.Sym.Scer\UAS] is approximately 60% of that of controls at 1 hour after the start of mating. (Avila and Wolfner, 2009)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Acp36DE^{dsRNA.Sym.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Avila and Wolfner, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(37): 15796--15800 Acp36DE is required for uterine conformational changes in mated Drosophila females. [FBrf0208975]

Allele Dmel\Acp36DEdsRNA.Sym.Scer\UAS

Allele Dmel\Acp36DE^{dsRNA.Sym.Scer\UAS}