Allele Dmel\Iap2^7c

General Information
Symbol	Dmel\Iap2^7c	Species	D. melanogaster
Name		FlyBase ID	FBal0243966
Feature type	allele	Associated gene	Dmel\Iap2
Also Known As	Diap2^7c

Allele class	amorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Leulier et al., 2006)
Mutagen	P-element activity (Leulier et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EP}Iap2^G2326 (Leulier et al., 2006)
Nature of the lesion	Statement Reference Imprecise excision of the progenitor insertion resulting in a 1357 bp genomic deletion removing the start codon and the first 735 nt into the coding region of Iap2. The resultant protein lacks exon one and most of exon two. (Leulier et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	cell death defective \| conditional (Ribeiro et al., 2007) cell death defective \| conditional (with Df(2R)Exel7138) (Ribeiro et al., 2007) immune response defective (Leulier et al., 2006, Meinander et al., 2012) immune response defective (with Df(2R)Exel7138) (Leulier et al., 2006) immune response defective (with Iap2^7a) (Leulier et al., 2006) radiation sensitive (Ribeiro et al., 2007) radiation sensitive (with Df(2R)Exel7138) (Ribeiro et al., 2007) viable (Leulier et al., 2006) viable (with Df(2R)Exel7138) (Leulier et al., 2006)
Phenotype Manifest In
	wing disc (Ribeiro et al., 2007) wing disc (with Df(2R)Exel7138) (Ribeiro et al., 2007)
Detailed Description
	Statement Reference Mutant flies show greatly reduced survival compared to wild type after septic injury with Erwinia carotovora carotovora 15. (Meinander et al., 2012) Iap2[7c]/Df(2R)Exel7138 or Iap2[7c] homozygous third instar larvae subjected to sublethal doses of x-ray irradiation (1.5 Gy) display a marked increase in wing imaginal disc apoptosis compared to wild-type larvae. (Ribeiro et al., 2007) 86% of Iap2[7c]/Df(2R)Exel7138 animals survive embryogenesis and develop normally, however animals are acutely sensitive to infections. Iap2[7c]/Df(2R)Exel7138 animals are highly susceptible to infection by gram-negative bacteria, but not to gram-positive bacteria and fungi. Iap2[7c]/Df(2R)Exel7138 flies die within 25 hours of septic injury with gram-negative bacteria, and almost identical susceptibility is observed for Iap2[7c], or Iap2[7c]/Iap2[7a] transheterozygous mutant animals. (Leulier et al., 2006)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Iap2^7c is rescued by Iap2^+t3.3 (Ribeiro et al., 2007) Iap2^7c is rescued by Iap2^{BIR2mut.Scer\UAS}/Scer\GAL4^c564 (Meinander et al., 2012) Iap2^7c is rescued by Iap2^{BIR3mut.Scer\UAS}/Scer\GAL4^c564 (Meinander et al., 2012) Iap2^7c is rescued by Iap2^Scer\UAS.cLa/Iap2^Scer\UAS.cLa (Leulier et al., 2006) Iap2^7c is rescued by Iap2^Scer\UAS.cLa/Scer\GAL4^da.G32/Iap2^Scer\UAS.cLa (Leulier et al., 2006, Meinander et al., 2012) Iap2^7c is rescued by Scer\GAL4^Act5C.PU/Iap2^Scer\UAS.cLa/Iap2^Scer\UAS.cLa (Leulier et al., 2006) Iap2^7c is rescued by Scer\GAL4^c564/Iap2^{Scer\UAS.attB} (Meinander et al., 2012)
Not rescued by	Iap2^7c is not rescued by Scer\GAL4^c564/Iap2^{BIR23mut.Scer\UAS} (Meinander et al., 2012) Iap2^7c is not rescued by Scer\GAL4^da.G32/Iap2^{RINGmut.Scer\UAS} (Meinander et al., 2012)
Comments	Expression of Iap2[RINGmut.Scer\UAS] under the control of Scer\GAL4[da.G32] fails to rescue the reduced survival after septic injury with Erwinia carotovora carotovora which is seen in Iap2[7c] flies. Expression of Iap2[Scer\UAS.cLa] under the control of Scer\GAL4[da.G32] rescues the reduced survival after septic injury with Erwinia carotovora carotovora 15 which is seen in Iap2[7c] flies. Expression of either Iap2[Scer\UAS.attB], Iap2[BIR2mut.Scer\UAS] or Iap2[BIR3mut.Scer\UAS] under the control of Scer\GAL4[c564] rescues the reduced survival after septic injury with Erwinia carotovora carotovora 15 which is seen in Iap2[7c] flies. Expression of Iap2[BIR23mut.Scer\UAS] under the control of Scer\GAL4[da.G32] fails to rescue the reduced survival after septic injury with Erwinia carotovora carotovora 15 which is seen in Iap2[7c] flies. (Meinander et al., 2012) Enhanced sensitivity of Iap2[7c] homozygotes to x-ray irradiation is rescued by Iap2[+t3.3]. (Ribeiro et al., 2007) Leaky, low-level expression of Iap2[Scer\UAS.cLa] in a Iap2[7c] mutant background is sufficient to completely rescue the immunodeficient phenotype observed in Iap2[7c] mutant animals in response to infection by gram-negative bacteria. Iap2[7c] mutant animals expressing Iap2[Scer\UAS.cLa], under the control of Scer\GAL4[Act5C] or Scer\GAL4[da.G32], are fully resistant to infection by gram-negative bacterium and survive microbial exposure at a similar rate to wild-type. (Leulier et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Diap2^7c (Meinander et al., 2012) diap2^7c (Leulier et al., 2006, Ribeiro et al., 2007, Paquette et al., 2010) Iap2^7c
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Meinander et al., 2012, EMBO J. 31(12): 2770--2783 Ubiquitylation of the initiator caspase DREDD is required for innate immune signalling. [FBrf0218688] Paquette et al., 2010, Mol. Cell 37(2): 172--182 Caspase-Mediated Cleavage, IAP Binding, and Ubiquitination: Linking Three Mechanisms Crucial for Drosophila NF-kappaB Signaling. [FBrf0209837] Ribeiro et al., 2007, J. Cell Biol. 179(7): 1467--1480 DIAP2 functions as a mechanism-based regulator of drICE that contributes to the caspase activity threshold in living cells. [FBrf0201142] Leulier et al., 2006, Mol. Cell. Biol. 26(21): 7821--7831 The Drosophila inhibitor of apoptosis protein DIAP2 functions in innate immunity and is essential to resist gram-negative bacterial infection. [FBrf0192381]

Allele Dmel\Iap27c

Allele Dmel\Iap2^7c