Co-expression of cindrdsRNA.PC.PD.Scer\UAS and cindrdsRNA.Scer\UAS under the control of Scer\GAL4GR1 results in a significant increase in the number of binucleate follicle cells compared to control egg chambers. The increase in binucleate follicle cells is accompanied by a significant decrease in the average number of follicle cell intercellular bridges per nucleus.
Ubiquitous expression of cindrdsRNA.Scer\UAS under the control of Scer\GAL4tub leads to embryonic and early larval lethality.
Expression of cindrdsRNA.Scer\UAS and cindrdsRNA.PC.PD.Scer\UAS in the eye for at least 24 hours (under the control of Scer\GAL4GMR.PF) does not generate any developmental defects.
Initial recruitment of 1[o] cells in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS, cindrdsRNA.Scer\UAS eyes commences normally and many, though not all, fledgling 1[o] cells successfully encircle a central cone cell quartet. However, these emerging 1[o] cells commonly fail to maintain their niches: 1[o] cells often lose contact with their partners, a loss typically coupled with dissolution of their shared junction. As a result, one proto-1[o] cell is often replaced by another cell to generate a new 1[o] cell. Remarkably, this instability of the 1[o]-1[o] interface continues throughout the stages of interommatidial precursor cell patterning hours after 1[o] cells have normally completed their movements.
Expression of cindrdsRNA.Scer\UAS in the pupal eye (under the control of Scer\GAL4GMR.PF) results in misplacement of cells, misorientation of pattern elements within the ommatidial field, and a variable number of pigment cells. An additional one to two pigment cells surround approximately 16% of Scer\GAL4GMR.PF>cindrdsRNA.Scer\UAS ommatidia. Patterning of the interommatidial precursor cells is delayed or lost in these flies.
Small clonal patches of 1[o] cells expressing cindrdsRNA.PC.PD.Scer\UAS and cindrdsRNA.Scer\UAS under the control of Scer\GAL4Scer\FRT.Act5C are consistently smaller than their wild-type neighbours. Paired cindrdsRNA.PC.PD.Scer\UAS 1[o] cells frequently retract, permitting a neighbouring wild-type interommatidial precursor cell direct contact with the central cone cells and often leads to an ectopic third 1[o] cell.
Strongly reducing cindr activity (in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS, cindrdsRNA.Scer\UAS mutants) has a marked effect on F-actin during interommatidial precursor cell patterning: at 28 hours APF, the density of F-actin fibrils and spikes filling the cytoplasm of interommatidial precursor cells is markedly increased. At later stages of patterning, a more dense and disorganized cytoskeletal network is observed in interommatidial precursor cells and the radiating arrangement of actin fibrils in 1[o] cells absent at 41 hours APF. Within cone cells, F-actin levels are mildly elevated at 28 hours APF, and distinct F-actin spikes fail to radiate from cell membranes.