Allele Dmel\cindr^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\cindr^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0243992
Feature type	allele	Associated gene	Dmel\cindr

Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Johnson et al., 2008) in vitro construct - RNAi (Johnson et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat (IR) that targets all known (3) isoforms of cindr. (Johnson et al., 2008)
Carried in construct	P{UAS-cindr.IR} (Haglund et al., 2010, Johnson et al., 2008, Johnson and Cagan, 2009, Quinones et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage, with Scer\GAL4^tub.PU (Johnson et al., 2008) lethal \| first instar larval stage, with Scer\GAL4^tub.PU (Johnson et al., 2008) planar polarity defective, with Scer\GAL4^GMR.PF (Johnson et al., 2008)
Phenotype Manifest In
	actin cytoskeleton, with Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.Scer\UAS} (Johnson et al., 2008) border follicle cell, with Scer\GAL4³⁰⁶ (Quinones et al., 2010) eye \| pupal stage, with Scer\GAL4^GMR.PF (Johnson and Cagan, 2009) follicle cell, with Scer\GAL4^GR1, cindr^{dsRNA.PC.PD.Scer\UAS} (Haglund et al., 2010) intercellular bridge, with Scer\GAL4^GR1, cindr^{dsRNA.PC.PD.Scer\UAS} (Haglund et al., 2010) ommatidium, with Scer\GAL4^GMR.PF (Johnson et al., 2008) ommatidium \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Johnson et al., 2008) ommatidium \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C}, cindr^{dsRNA.PC.PD.Scer\UAS} (Johnson et al., 2008) pigment cell, with Scer\GAL4^GMR.PF (Johnson et al., 2008) primary pigment cell, with Scer\GAL4^GMR.PF, cindr^{dsRNA.PC.PD.Scer\UAS} (Johnson et al., 2008)
Detailed Description
	Statement Reference Co-expression of cindr[dsRNA.PC.PD.Scer\UAS] and cindr[dsRNA.Scer\UAS] under the control of Scer\GAL4[GR1] results in a significant increase in the number of binucleate follicle cells compared to control egg chambers. The increase in binucleate follicle cells is accompanied by a significant decrease in the average number of follicle cell intercellular bridges per nucleus. (Haglund et al., 2010) Expression of cindr[dsRNA.Scer\UAS] under the control of Scer\GAL4[306] results in a weak border cell migration defect in the egg chamber. (Quinones et al., 2010) Expression of cindr[dsRNA.Scer\UAS] driven by Scer\GAL4[GMR.PF] results in patterning defect in pupal eyes. (Johnson and Cagan, 2009) Ubiquitous expression of cindr[dsRNA.Scer\UAS] under the control of Scer\GAL4[tub] leads to embryonic and early larval lethality. Expression of cindr[dsRNA.Scer\UAS] and cindr[dsRNA.PC.PD.Scer\UAS] in the eye for at least 24 hours (under the control of Scer\GAL4[GMR.PF]) does not generate any developmental defects. Initial recruitment of 1[o] cells in Scer\GAL4[GMR.PF]>cindr[dsRNA.PC.PD.Scer\UAS], cindr[dsRNA.Scer\UAS] eyes commences normally and many, though not all, fledgling 1[o] cells successfully encircle a central cone cell quartet. However, these emerging 1[o] cells commonly fail to maintain their niches: 1[o] cells often lose contact with their partners, a loss typically coupled with dissolution of their shared junction. As a result, one proto-1[o] cell is often replaced by another cell to generate a new 1[o] cell. Remarkably, this instability of the 1[o]-1[o] interface continues throughout the stages of interommatidial precursor cell patterning hours after 1[o] cells have normally completed their movements. Expression of cindr[dsRNA.Scer\UAS] in the pupal eye (under the control of Scer\GAL4[GMR.PF]) results in misplacement of cells, misorientation of pattern elements within the ommatidial field, and a variable number of pigment cells. An additional one to two pigment cells surround approximately 16% of Scer\GAL4[GMR.PF]>cindr[dsRNA.Scer\UAS] ommatidia. Patterning of the interommatidial precursor cells is delayed or lost in these flies. Small clonal patches of 1[o] cells expressing cindr[dsRNA.PC.PD.Scer\UAS] and cindr[dsRNA.Scer\UAS] under the control of Scer\GAL4[Scer\FRT.Act5C] are consistently smaller than their wild-type neighbours. Paired cindr[dsRNA.PC.PD.Scer\UAS] 1[o] cells frequently retract, permitting a neighbouring wild-type interommatidial precursor cell direct contact with the central cone cells and often leads to an ectopic third 1[o] cell. Strongly reducing cindr activity (in Scer\GAL4[GMR.PF]>cindr[dsRNA.PC.PD.Scer\UAS], cindr[dsRNA.Scer\UAS] mutants) has a marked effect on F-actin during interommatidial precursor cell patterning: at 28 hours APF, the density of F-actin fibrils and spikes filling the cytoplasm of interommatidial precursor cells is markedly increased. At later stages of patterning, a more dense and disorganized cytoskeletal network is observed in interommatidial precursor cells and the radiating arrangement of actin fibrils in 1[o] cells absent at 41 hours APF. Within cone cells, F-actin levels are mildly elevated at 28 hours APF, and distinct F-actin spikes fail to radiate from cell membranes. (Johnson et al., 2008)
External Data
Linkouts
Interactions
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Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, cindr^{dsRNA.Scer\UAS} has eye \| pupal stage phenotype, enhanceable by ena[+]/ena^GC1/Scer\GAL4^GMR.PF (Johnson and Cagan, 2009)
Suppressed by
Statement Reference Scer\GAL4³⁰⁶, cindr^{dsRNA.Scer\UAS} has border follicle cell phenotype, suppressible by mim^null (Quinones et al., 2010)
Suppressor of
Statement Reference cindr^{dsRNA.Scer\UAS}/Scer\GAL4³⁰⁶ is a suppressor of border follicle cell phenotype of mim^null (Quinones et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Expression of cindr[dsRNA.Scer\UAS] under the control of Scer\GAL4[306] in a mim[null] background restores the ability of border cells in the egg chamber to migrate. (Quinones et al., 2010) The pupal eye patterning defect phenotype caused by the expression of cindr[dsRNA.Scer\UAS] driven by Scer\GAL4[GMR.PF] is enhanced by ena[GC1]/+. (Johnson and Cagan, 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	cindr^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Haglund et al., 2010, Curr. Biol. 20(10): 944--950 Cindr interacts with anillin to control cytokinesis in Drosophila melanogaster. [FBrf0210868] Quinones et al., 2010, J. Cell Biol. 189(2): 353--367 I-BAR protein antagonism of endocytosis mediates directional sensing during guided cell migration. [FBrf0210570] Johnson and Cagan, 2009, PLoS ONE 4(9): e7008 A quantitative method to analyze Drosophila pupal eye patterning. [FBrf0208849] Johnson et al., 2008, J. Cell Biol. 180(6): 1191--1204 The Drosophila CD2AP/CIN85 orthologue Cindr regulates junctions and cytoskeleton dynamics during tissue patterning. [FBrf0204348]

Allele Dmel\cindrdsRNA.Scer\UAS

Allele Dmel\cindr^{dsRNA.Scer\UAS}