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General Information
Symbol
Dmel\cindrdsRNA.PC.PD.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0243994
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-cindrRNAi2.21+23, cindr-IR2
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of an inverted repeat (IR) that specifically targets the long (PC) and intermediate (PD) isoforms of cindr.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of cindrdsRNA.PC.PD.Scer\UAS under the control of Scer\GAL4GMR.PU results in a perturbed ommatidial lattice and patterning defects in the pupal retina, the number of primary pigment cells is also mildly increased.

Co-expression of cindrdsRNA.Scer\UAS and cindrdsRNA.PC.PD.Scer\UAS under the control of Scer\GAL4GMR.PF results in defects in the position and shape of interommatidial precursor cells, primary primary pigment cells and cone cells.

Expression of cindrdsRNA.PC.PD.Scer\UAS under the control of Scer\GAL4GMR.PF leads to multiple errors in the final arrangement of ommatidial cells.

Co-expression of cindrdsRNA.PC.PD.Scer\UAS and cindrdsRNA.Scer\UAS under the control of Scer\GAL4GR1 results in a significant increase in the number of binucleate follicle cells compared to control egg chambers. The increase in binucleate follicle cells is accompanied by a significant decrease in the average number of follicle cell intercellular bridges per nucleus.

Expression of cindrdsRNA.PC.PD.Scer\UAS under the control of Scer\GAL4306 results in a weak border cell migration defect in the egg chamber.

Border cells expressing cindrdsRNA.PC.PD.Scer\UAS under the control of Scer\GAL4306 show a slightly reduced rate of uptake of the lipophilic dye FM4-64 compared to wild-type controls.

Expression of cindrdsRNA.PC.PD.Scer\UAS driven by Scer\GAL4GMR.PF results in patterning defect in pupal eyes.

Ubiquitous expression of cindrdsRNA.PC.PD.Scer\UAS under the control of Scer\GAL4tub leads to embryonic and early larval lethality.

Expression of cindrdsRNA.Scer\UAS and cindrdsRNA.PC.PD.Scer\UAS in the eye for at least 24 hours (under the control of Scer\GAL4GMR.PF) does not generate any developmental defects.

Expression of cindrdsRNA.PC.PD.Scer\UAS in the eye for at least 24 hours (under the control of Scer\GAL4GMR.PF) does not generate any developmental defects.

Expression of cindrdsRNA.PC.PD.Scer\UAS in the pupal eye (under the control of Scer\GAL4GMR.PF) results in misplacement of cells, misorientation of pattern elements within the ommatidial field, and a variable number of pigment cells. An additional one to two pigment cells surround approximately 46% of Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS ommatidia. The most posterior fifth of Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS retinae show more severe defects: 81% of ommatidia exhibit a shortfall of one to five surround interommatidial precursor cells). Patterning of the interommatidial precursor cells is delayed or lost in these flies.

Initial recruitment of 1[o] cells in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS, cindrdsRNA.Scer\UAS eyes commences normally and many, though not all, fledgling 1[o] cells successfully encircle a central cone cell quartet. However, these emerging 1[o] cells commonly fail to maintain their niches: 1[o] cells often lose contact with their partners, a loss typically coupled with dissolution of their shared junction. As a result, one proto-1[o] cell is often replaced by another cell to generate a new 1[o] cell. Remarkably, this instability of the 1[o]-1[o] interface continues throughout the stages of interommatidial precursor cell patterning hours after 1[o] cells have normally completed their movements.

A failure to maintain proper position and stable junctions is seen in the emerging arrays of 2[o] and 3[o] pigment cells in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutant retinae. Excessive switching of cell-cell contacts, severe alterations in apical cell profiles and a failure of interommatial precursor cells to stably rearrange stepwise into single file is observed in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutant developing eyes. The result is a failure to achieve coherent patterning.

Small clonal patches of 1[o] cells expressing cindrdsRNA.PC.PD.Scer\UAS and cindrdsRNA.Scer\UAS under the control of Scer\GAL4Scer\FRT.Act5C are consistently smaller than their wild-type neighbours. Paired cindrdsRNA.PC.PD.Scer\UAS 1[o] cells frequently retract, permitting a neighbouring wild-type interommatidial precursor cell direct contact with the central cone cells and often leads to an ectopic third 1[o] cell.

Depletion of cindr through expression of cindrdsRNA.PC.PD.Scer\UAS in the adult thorax, under the control of Scer\GAL4ap-md544 results in macrochaetea that are missing, hooked, twisted, thickened, or stunted.

Strongly reducing cindr activity (in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS, cindrdsRNA.Scer\UAS mutants) has a marked effect on F-actin during interommatidial precursor cell patterning: at 28 hours APF, the density of F-actin fibrils and spikes filling the cytoplasm of interommatidial precursor cells is markedly increased. At later stages of patterning, a more dense and disorganized cytoskeletal network is observed in interommatidial precursor cells and the radiating arrangement of actin fibrils in 1[o] cells absent at 41 hours APF. Within cone cells, F-actin levels are mildly elevated at 28 hours APF, and distinct F-actin spikes fail to radiate from cell membranes.

External Data
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Phenotypic Class
Enhanced by
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Suppressed by
Phenotype Manifest In
Enhanced by
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Suppressed by
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Suppressor of
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Additional Comments
Genetic Interactions
Statement
Reference

The patterning defects and mild supernumerary primary pigment cells phenotype observed in pupal retina of flies expressing cindrdsRNA.PC.PD.Scer\UAS under the control of Scer\GAL4GMR.PU can be significantly enhanced by combination with either Vav2 or Vav3 in heterozygous state.

CblF165/+ enhances the mis-patterning of interommatidial precursor cells, primary primary pigment cells and cone cells caused by co-expression of cindrdsRNA.Scer\UAS and cindrdsRNA.PC.PD.Scer\UAS under the control of Scer\GAL4GMR.PF.

The ommatidial patterning errors resulting from the expression of cindrdsRNA.PC.PD.Scer\UAS via Scer\GAL4GMR.PF are strongly increased in ArfGAP3e01250 heterozygotes.

The ommatidial patterning errors resulting from the expression of cindrdsRNA.PC.PD.Scer\UAS via Scer\GAL4GMR.PF are strongly increased in Asap1KG03963 heterozygotes.

Overexpression of ArfGAP3d00510 partially suppresses the ommatidial patterning defects resulting from th expression of cindrdsRNA.PC.PD.Scer\UAS driven by Scer\GAL4GMR.PF.

The ommatidial patterning errors resulting from the expression of cindrdsRNA.PC.PD.Scer\UAS via Scer\GAL4GMR.PF are strongly enhanced in sizT1510 heterozygotes.

The ommatidial patterning errors resulting from the expression of cindrdsRNA.PC.PD.Scer\UAS via Scer\GAL4GMR.PF are strongly enhanced in sizT1032 heterozygotes.

The ommatidial patterning errors resulting from the expression of cindrdsRNA.PC.PD.Scer\UAS via Scer\GAL4GMR.PF are strongly enhanced in Efa6KO1 heterozygotes.

The ommatidial patterning errors resulting from the expression of cindrdsRNA.PC.PD.Scer\UAS via Scer\GAL4GMR.PF are strongly enhanced in Efa6c00953 heterozygotes.

The ommatidial patterning errors resulting from the expression of cindrdsRNA.PC.PD.Scer\UAS via Scer\GAL4GMR.PF are strongly enhanced in Arf51FΔKG1 heterozygotes.

The ommatidial patterning errors resulting from the expression of cindrdsRNA.PC.PD.Scer\UAS via Scer\GAL4GMR.PF are strongly enhanced in Arf51FKG02753 heterozygotes.

The ommatidial patterning errors resulting from the expression of cindrdsRNA.PC.PD.Scer\UAS via Scer\GAL4GMR.PF are strongly enhanced in Arf51FEP2612 heterozygotes.

Expression of cindrdsRNA.PC.PD.Scer\UAS under the control of Scer\GAL4306 in a mimnull background restores the ability of border cells in the egg chamber to migrate.

The pupal eye patterning defect phenotype caused by the expression of cindrdsRNA.PC.PD.Scer\UAS driven by Scer\GAL4GMR.PF is enhanced by enaGC1/+.

The Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mispatterning phenotype is enhanced in a heterozygous shgR69 background. In addition, large patches of black tissue are observed in the eyes of 68.3% of these adult flies; this is a significant enhancement of the penetrance and extent of degenerative tissue that is observed in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutant flies (12.6%).

Ectopic expression of shgScer\UAS.T:Avic\GFP-rs strongly suppresses the Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mispatterning phenotype and reduces the distance between interommatidial precursor cells.

Pupae heterozygous for hbs361, hbs459, In(1)rst3, or rst6 exhibit mild but consistent suppression of the Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS phenotype. The prevalence of cone and 1[o] cell defects is decreased and the number of 2[o] and 3[o] cells is reduced to slightly below normal.

A cpascrd2 background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A cpascrd1 background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A cpb6.15 background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A cpbF19 background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A tsrN96A heterozygous background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A tsrN121 heterozygous background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A chic221 heterozygous background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

An Abl2 heterozygous background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A WASp3 heterozygous background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A Df(3R)3450 heterozygous background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A Rho172R heterozygous background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

A Rac1J11 Rac2Δ MtlΔ heterozygous background enhances the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

Ectopic expression of Arp66BEP3640 suppresses the patterning defects found in Scer\GAL4GMR.PF>cindrdsRNA.PC.PD.Scer\UAS mutants. The mean interommatidial precursor cell number and the number of cone and/or 1[o] cell errors is increased in these double mutants.

Xenogenetic Interactions
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Complementation and Rescue Data
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Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
cindrdsRNA.PC.PD.Scer\UAS
cindrdsRNA.PC.PD.UAS
Name Synonyms
Secondary FlyBase IDs
    References (9)