FB2025_01 , released February 20, 2025
Allele: Dmel\atlK51A.UAS.Tag:MYC
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General Information
Symbol
Dmel\atlK51A.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0244005
Feature type
allele
Associated gene
Dmel\atl
Associated Insertion(s)
Carried in Construct
P{UAS-atl.myc.K51A}
Key Links
Transgenic product class
dominant_negative_variant
(Summerville et al., 2016)
missense_variant
(Orso et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Summerville et al., 2016)
missense_variant
(Orso et al., 2009)
Mutagen
in vitro construct
(Orso et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-atl.myc.K51A}
Cytology
Description

Encodes a dominant negative form of atl.

(Summerville et al., 2016)

UASt regulatory sequences drive expression of a mutant form of atl carrying a Lys51Ala substitution in the GTP-binding domain tagged with Tag:MYC.

(Orso et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
atl
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of atlK51A.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4nSyb.PS decreases endoplasmic reticulum (ER) tubule crossbridges (and ER tubules become wavy) in motor neuron axon initial segments and a diffuse ER fragmentation at motor nerve terminals of third instar larvae.

      Expression of atlK51A.Scer\UAS.T:Hsap\MYC driven by Scer\GAL4Toll-6-D42 or Scer\GAL4nSyb.PS (but not Scer\GAL4Mef2.PR) (at 0.1 mM Ca[2+]) significantly reduces EJP amplitude at the third instar larval NMJ; reduction in EJP corresponds with a decrease in the frequency of successful synaptic transmission.

      (Summerville et al., 2016)

      Overexpression of atlK51A.Scer\UAS.T:Hsap\MYC] driven by Scer\GAL4GMR.PF has no phenotypic effects.

      (Orso et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4unspecified/atlK51A.UAS.Tag:MYC fails to rescue atl1

      (Orso et al., 2009)
      Comments

      atlK51A.Scer\UAS.T:Hsap\MYC] driven by Scer\GAL4unspecified is unable to rescue the endoplasmic reticulum fragmentation phenotype of homozygous atl1 mutant neurons.

      (Orso et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      atlK51A.Scer\UAS.T:Hsap\MYC
      atlK51A.Scer\UAS.T:Hsap\MYC</up>
      atlK51A.UAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (2)