FlyBase Allele Report: Scer\GAL4[mex1.2.1]

General Information

Symbol

Scer\GAL4^mex1.2.1

Species

S. cerevisiae

Name

FlyBase ID

FBal0244042

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{mex1-GAL4.2.1}

Also Known As

Mex-Gal4, mex1-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Phillips and Thomas, 2006)

Progenitor genotype

Carried in construct

P{mex1-GAL4.2.1}

Cytology

Description

A 2.1kb promoter fragment from the mex1 locus is fused upstream of sequence encoding a GAL4 driver.

(Phillips and Thomas, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

mex1

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval midgut

(Sieber and Thummel, 2012, Tennessen et al., 2011, Mazock et al., 2010)

larval midgut enterocyte

(Redhai et al., 2020)

third instar larval stage

embryonic/larval midgut

(Binks et al., 2010)

adult stage

adult midgut

(Palu and Thummel, 2016, Chen et al., 2014)

enterocyte of posterior adult midgut epithelium

(Hudry et al., 2016)

Additional Information

Statement

Reference

Drives expression in the midgut.

(FlyBase Curators, 2017)

Marker for

enterocyte of posterior adult midgut epithelium

(Hudry et al., 2016)

Reflects expression of

mex1

(Palu and Thummel, 2016, Chen et al., 2014, Sieber and Thummel, 2012, Tennessen et al., 2011, Binks et al., 2010, Mazock et al., 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with pHCl-2^KK106835

(Redhai et al., 2020)

abnormal developmental rate, with pHCl-2^NIG.11340R

(Redhai et al., 2020)

abnormal immune response | adult stage, with acs^KK111566

(Socha et al., 2023)

abnormal oxidative stress response, with Psn^KK109329

(Southon et al., 2013)

chemical resistant, with Psn^KK109329

(Southon et al., 2013)

chemical sensitive, with ATP7^UAS.Tag:FLAG

(Southon et al., 2013)

chemical sensitive | adult stage, with Hnf4^NIG.9310R

(Vonolfen et al., 2024)

decreased cell size | adult stage, with Pex5^GD14972

(Pinelli et al., 2024)

decreased cell size | larval stage | somatic clone, with detr^NIG.14435R

(Nicolson et al., 2024)

decreased size | larval stage, with detr^GD8324

(Nicolson et al., 2024)

decreased size | larval stage, with detr^NIG.14435R

(Nicolson et al., 2024)

hyperplasia, with key^GD1249

(Chen et al., 2014)

increased cell number | adult stage, with cbt^UAS.cMa

(Mukherjee et al., 2021)

increased cell number | adult stage, with mir-958^{sponge.V2.UAS.mCherry}

(Mukherjee et al., 2021)

increased cell number | adult stage, with Pex5^GD14972

(Pinelli et al., 2024)

increased mortality | chemical conditional, with Hnf4^NIG.9310R

(Vonolfen et al., 2024)

increased occurrence of cell division | adult stage, with Hnf4^NIG.9310R

(Vonolfen et al., 2024)

lethal | chemical conditional, with ATP7^UAS.Tag:FLAG

(Zhang et al., 2020)

lethal | larval stage, with Syx5^GD1743

(Norgate et al., 2010)

lethal | P-stage, with Syx5^GD1743

(Norgate et al., 2010)

lethal - all die before end of larval stage, with β-Spec^{UAS.cMa.Tag:MYC}

(Mazock et al., 2010)

lethal - all die before end of P-stage, with Cyp18a1^UAS.cGa

(Scanlan and Robin, 2024)

long lived | female, with snoRNA:Ψ28S-1153^UAS.cSa

(Soulé et al., 2020)

partially lethal, with pHCl-2^KK106835

(Redhai et al., 2020)

some die during larval stage, with pHCl-2^KK106835

(Redhai et al., 2020)

some die during larval stage, with β-Spec^{UAS.cMa.Tag:MYC}

(Mazock et al., 2010)

viable, with Psn^KK109329

(Southon et al., 2013)

viable, with wall^EY20330

(Scanlan and Robin, 2024)

viable, with wall^UAS.pU

(Scanlan and Robin, 2024)

viable | larval stage, with Ae2^GD2397

(Dubreuil et al., 2010)

Phenotype Manifest In

adult enterocyte, with Pex5^GD14972

(Pinelli et al., 2024)

adult enterocyte | conditional, with acs^KK111566

(Socha et al., 2023)

adult gut, with Pex5^GD14972

(Pinelli et al., 2024)

adult gut, with yki^UAS.cUa

(Pinelli et al., 2024)

adult gut | female, with snoRNA:Ψ28S-1153^UAS.cSa

(Soulé et al., 2020)

adult midgut, with Hnf4^NIG.9310R

(Vonolfen et al., 2024)

adult midgut, with key^GD1249

(Chen et al., 2014)

adult midgut, with Pex5^GD14972

(Pinelli et al., 2024)

adult midgut epithelium, with cbt^UAS.cMa

(Mukherjee et al., 2021)

adult midgut epithelium, with mir-958^{sponge.V2.UAS.mCherry}

(Mukherjee et al., 2021)

adult midgut interstitial cell, with Hnf4^RNAi.UAS

(Vonolfen et al., 2024)

adult midgut region R1, with Hnf4^RNAi.UAS

(Vonolfen et al., 2024)

adult midgut region R2, with Hnf4^RNAi.UAS

(Vonolfen et al., 2024)

adult midgut region R2 | nutrition conditional, with Hnf4^HMC05862

(Vonolfen et al., 2024)

adult midgut region R3, with CG2772^KK104580

(Vonolfen et al., 2024)

adult midgut region R3, with CG3635^GD1804

(Vonolfen et al., 2024)

adult midgut region R3, with CG31089^GD287

(Vonolfen et al., 2024)

adult midgut region R3, with CG31091^GD286

(Vonolfen et al., 2024)

adult midgut region R3, with foxo^KK108485

(Vonolfen et al., 2024)

adult midgut region R3, with Hnf4^HMC05862

(Vonolfen et al., 2024)

adult midgut region R3, with Hnf4^NIG.9310R

(Vonolfen et al., 2024)

adult midgut region R3, with Hnf4^RNAi.UAS

(Vonolfen et al., 2024)

adult midgut region R3, with mag^KK103835

(Vonolfen et al., 2024)

adult midgut region R3, with Plc21C^GD11359

(Vonolfen et al., 2024)

adult midgut region R3, with Pld^GD7487

(Vonolfen et al., 2024)

adult midgut region R3, with Rab5^KK102895

(Vonolfen et al., 2024)

adult midgut region R4 | nutrition conditional, with Hnf4^HMC05862

(Vonolfen et al., 2024)

adult midgut region R5, with CG31089^GD287

(Vonolfen et al., 2024)

adult midgut region R5, with CG31091^GD286

(Vonolfen et al., 2024)

adult midgut region R5, with Hnf4^HMC05862

(Vonolfen et al., 2024)

adult midgut region R5, with Hnf4^NIG.9310R

(Vonolfen et al., 2024)

adult midgut region R5, with Hnf4^RNAi.UAS

(Vonolfen et al., 2024)

adult midgut region R5, with Rab5^KK102895

(Vonolfen et al., 2024)

embryonic/larval copper cell, with Gp93^NIG.5520R

(Maynard et al., 2010)

embryonic/larval midgut cell | larval stage | somatic clone, with detr^NIG.14435R

(Nicolson et al., 2024)

gastric caecum | larval stage, with detr^GD8324

(Nicolson et al., 2024)

gastric caecum | larval stage, with detr^NIG.14435R

(Nicolson et al., 2024)

lipid droplet | adult stage, with CG2772^KK104580

(Vonolfen et al., 2024)

lipid droplet | adult stage, with CG3635^GD1804

(Vonolfen et al., 2024)

lipid droplet | adult stage, with CG31089^GD287

(Vonolfen et al., 2024)

lipid droplet | adult stage, with CG31091^GD286

(Vonolfen et al., 2024)

lipid droplet | adult stage, with foxo^KK108485

(Vonolfen et al., 2024)

lipid droplet | adult stage, with Hnf4^HMC05862

(Vonolfen et al., 2024)

lipid droplet | adult stage, with Hnf4^NIG.9310R

(Vonolfen et al., 2024)

lipid droplet | adult stage, with Hnf4^RNAi.UAS

(Vonolfen et al., 2024)

lipid droplet | adult stage, with mag^KK103835

(Vonolfen et al., 2024)

lipid droplet | adult stage, with Plc21C^GD11359

(Vonolfen et al., 2024)

lipid droplet | adult stage, with Pld^GD7487

(Vonolfen et al., 2024)

lipid droplet | adult stage, with Rab5^KK102895

(Vonolfen et al., 2024)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pex5^GD14972, Scer\GAL4^mex1.2.1 has decreased cell size | adult stage phenotype, suppressible by yki^UAS.cUa, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

ATP7^UAS.Tag:FLAG, Scer\GAL4^mex1.2.1 has chemical sensitive phenotype, suppressible by Psn^KK109329, Scer\GAL4^mex1.2.1

(Southon et al., 2013)

Suppressor of

Statement

Reference

lt^RNAi.UAS.cUa/Scer\GAL4^mex1.2.1 is a suppressor of decreased size | larval stage phenotype of detr¹

(Nicolson et al., 2024)

yki^UAS.cUa, Scer\GAL4^mex1.2.1 is a suppressor of decreased cell size | adult stage phenotype of Pex5^GD14972, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

Psn^KK109329, Scer\GAL4^mex1.2.1 is a suppressor of chemical sensitive phenotype of ATP7^UAS.Tag:FLAG, Scer\GAL4^mex1.2.1

(Southon et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pex5^GD14972, Scer\GAL4^mex1.2.1 has adult gut phenotype, suppressible by yki^UAS.cUa, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

Pex5^GD14972, Scer\GAL4^mex1.2.1 has adult gut phenotype, suppressible by mag^UAS.cSa, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

Pex5^GD14972, Scer\GAL4^mex1.2.1 has adult midgut phenotype, suppressible by yki^UAS.cUa, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

Pex5^GD14972, Scer\GAL4^mex1.2.1 has adult enterocyte phenotype, suppressible by yki^UAS.cUa, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

Hnf4^NIG.9310R, Scer\GAL4^mex1.2.1 has adult midgut region R3 phenotype, suppressible | partially by bmm^UAS.cUa, Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

Hnf4^NIG.9310R, Scer\GAL4^mex1.2.1 has lipid droplet | adult stage phenotype, suppressible | partially by bmm^UAS.cUa, Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

Hnf4^NIG.9310R, Scer\GAL4^mex1.2.1 has adult midgut region R5 phenotype, suppressible | partially by bmm^UAS.cUa, Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

Suppressor of

Statement

Reference

lt^RNAi.UAS.cUa/Scer\GAL4^mex1.2.1 is a suppressor of gastric caecum | larval stage phenotype of detr¹

(Nicolson et al., 2024)

yki^UAS.cUa, Scer\GAL4^mex1.2.1 is a suppressor of adult gut phenotype of Pex5^GD14972, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

mag^UAS.cSa, Scer\GAL4^mex1.2.1 is a suppressor of adult gut phenotype of Pex5^GD14972, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

yki^UAS.cUa, Scer\GAL4^mex1.2.1 is a suppressor of adult midgut phenotype of Pex5^GD14972, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

yki^UAS.cUa, Scer\GAL4^mex1.2.1 is a suppressor of adult enterocyte phenotype of Pex5^GD14972, Scer\GAL4^mex1.2.1

(Pinelli et al., 2024)

bmm^UAS.cUa, Scer\GAL4^mex1.2.1 is a suppressor | partially of adult midgut region R3 phenotype of Hnf4^NIG.9310R, Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

bmm^UAS.cUa, Scer\GAL4^mex1.2.1 is a suppressor | partially of lipid droplet | adult stage phenotype of Hnf4^NIG.9310R, Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

bmm^UAS.cUa, Scer\GAL4^mex1.2.1 is a suppressor | partially of adult midgut region R5 phenotype of Hnf4^NIG.9310R, Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Pex5^UAS.cPa/Scer\GAL4^mex1.2.1 rescues Pex5^GD14972

(Pinelli et al., 2024)

Scer\GAL4^mex1.2.1/pHCl-2^UAS.cPa rescues pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Zip89B^UAS.Tag:FLAG/Scer\GAL4^mex1.2.1 rescues Zip89B^Δ

(Richards et al., 2015)

Scer\GAL4^mex1.2.1/Hr96^UAS.cHa rescues Hr96¹

(Sieber and Thummel, 2009)

Fails to rescue

Scer\GAL4^mex1.2.1/Sdhaf3^UAS.cNa fails to rescue Sdhaf3^null

(Na et al., 2014)

Scer\GAL4^mex1.2.1/Mpc1^UAS.cBa fails to rescue Mpc1²/Mpc1¹

(Bricker et al., 2012)

Scer\GAL4^mex1.2.1/ERR^UAS.cTa fails to rescue ERR²/ERR¹

(Tennessen et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

91368

w¹¹¹⁸; P{mex1-GAL4.2.1}10-8

91367

P{mex1-GAL4.2.1}9-1, y¹ w¹¹¹⁸

91369

w¹¹¹⁸; TM2, P{mex1-GAL4.2.1}12-8/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^mex1.2.1

Name Synonyms

Secondary FlyBase IDs

References (58)

Report Sections

Open Close