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General Information
Symbol
Dmel\tefudsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0244112
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of an inverted repeat of a 642 bp fragment corresponding to exon 6 (nucleotides 1185-1827) of tefu.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
is exacerbated by Dl05151
is ameliorated by HDAC104556
is ameliorated by rad50EP1
is ameliorated by stgEY12388
is exacerbated by wrdA131
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies show expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4repo reduced climbing ability compared to wild type. In a countercurrent assay, 70% of the mutant flies are poor climbers, 16% moderate climbers and only 14% are good climbers (compared to wild-type values of 13% poor/moderate climbers and 87% good climbers). Flies which are poor climbers have significantly shorter longevity than good climbers, with the moderate climbers having intermediate longevity, suggesting that the difference in climbing ability between individual flies is due to differences in the severity of neurodegeneration.

Flies expressing tefudsRNA.Scer\UAS in glial cells under the control of Scer\GAL4repo exhibit climbing defects. They also exhibit significantly reduced longevity compared to controls, and unlike in wild type, holes are seen in the adult lamina and neuropil. Significantly more apoptotic (Caspase 3 positive) cells are seen than in wild type. At 7 days these are limited to neurons, but also include glial cells at 17 days. Mobility, longevity, brain morphology of apoptosis levels are not adversely affected when tefudsRNA.Scer\UAS is expressed in neurons under the control of Scer\GAL4elav.PLu, and flies have increased lifespan compared to controls. Lifespan and apoptosis levels are not affected when tefudsRNA.Scer\UAS is expressed using Scer\GAL4elav-C155, but a slight reduction in climbing ability is observed in young flies. No holes are seen in the brain, but several round structures with a darkly stained interior core are observed in the optic lobe.

Expression of tefudsRNA.Scer\UAS under the control of Scer\GAL4elav.PU results in neurodegeneration phenotypes. Swelling, fragmentation and loss of rhabdomeres in 3-day old adult eyes is observed.

Expression of tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU results in neurodegeneration phenotypes. Swelling, fragmentation and loss of rhabdomeres in 3-day old adult eyes is observed, and the extent of neurodegeneration becomes more severe with age. Eyes from these animals show a rough-eye phenotype.

Expression of tefudsRNA.Scer\UAS under the control of Scer\GAL4hs.PB during development (animals heat-shocked twice daily from egg-laying until day 3), or during adulthood (adults heat-shocked twice daily from day 3 to day 15), both result in degeneration of adult photoreceptor neurons.

Dividing photoreceptor neurons are observed posterior to the morphogenetic furrow in third instar larval eye discs expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU or Scer\GAL4elav.PU.

Reduced cell death directly posterior to the morphogenetic furrow, and increased cell death more posterior to the morphogenetic furrow is observed in third instar larval eye discs expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU. A small number of these dying cells are neurons.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Phenotype Manifest In
Enhanced by
Statement
Reference

Scer\GAL4GMR.PU, tefudsRNA.UAS has eye phenotype, enhanceable by Mekk1[+]/Mekk1EY02276

Scer\GAL4GMR.PU, tefudsRNA.UAS has eye phenotype, enhanceable by PP2A-B'[+]/wrdA131

Scer\GAL4GMR.PU, tefudsRNA.UAS has ommatidium phenotype, enhanceable by PP2A-B'[+]/wrdA131

Scer\GAL4GMR.PU, tefudsRNA.UAS has eye phenotype, enhanceable by Dl05151/Dl[+]

Suppressed by
NOT suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

stgEY12388/+ suppresses the rough-eye phenotype and increases the number of normal photoreceptors per ommatidium in flies expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU. Significantly reduced severity of progressive neurodegeneration is also observed in these animals.

Rpd304556/+ suppresses the rough-eye phenotype and increases the number of normal photoreceptors per ommatidium in flies expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU.

rad50EP1/+ suppresses the rough-eye phenotype and increases the number of normal photoreceptors per ommatidium in flies expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU.

Mekk1EY02276/+ enhances the rough-eye phenotype and reduces the number of normal photoreceptors per ommatidium in flies expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU. Increased incidence of ommatidium fusions are observed in these animals.

PP2A-B'A131/+ enhances the rough-eye phenotype and reduces the number of normal photoreceptors per ommatidium in flies expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU. Increased incidence of ommatidium fusions are observed in these animals.

Dl05151/+ enhances the rough-eye phenotype and reduces the number of normal photoreceptors per ommatidium in flies expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU. Increased incidence of ommatidium fusions are observed in these animals.

Expression of tefudsRNA.Scer\UAS under the control of Scer\GAL4elav.PU results in increased levels of photoreceptor cell division in third instar larval eye discs, which is suppressed by stgEY12388/+.

Xenogenetic Interactions
Statement
Reference

BacA\p35GMR.PH suppresses the rough eye and neurodegeneration phenotypes observed in flies expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU.

Expression of BacA\p35GMR.PH in third instar larval eye discs expressing tefudsRNA.Scer\UAS under the control of Scer\GAL4elav.PU does not suppress the increased levels of cell division observed when tefudsRNA.Scer\UAS is expressed alone.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
tefudsRNA.Scer\UAS
tefudsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (3)