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General Information
Symbol
Dmel\jnjhuc95E
Species
D. melanogaster
Name
FlyBase ID
FBal0244122
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant eyes (generated using the "EGUF" system) display morphological defects when larvae are raised on media containing 2 mmol caffeine/L and 3 mmol hydroxyurea/L. Larvae raised on 3 mmol hydroxyurea/L alone show a slightly rough eye, barely distinguishable from controls, whereas larvae raised on 2 mmol caffeine/L alone display a severe rough eye, indicating that mutants are primarily sensitive to caffeine. Larval ingestion of caffeine results in: adults with very small eyes and gross ommatidial defects; mutant eye discs that are small and exhibit patterning defects in the ommatidial preclusters; disruption to BrdU incorporation in mutant eye discs, though no gross changes in cell number are apparent; an increase in apoptotic cells anterior to the morphogenetic furrow.

Hemizygous mutants grown on 3 mmol hydroxyurea/L eclose at a similar frequency to controls, whereas hemizygous mutants raised on 2 mmol caffeine/L do not survive to adulthood and instead die during pupal stages. Hemizygous mutants are lethal when grown on concentrations of caffeine as low as 0.25 mmol/L.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
huc95E1
jnjhuc95E
Name Synonyms
Secondary FlyBase IDs
    References (2)