FB2025_01 , released February 20, 2025
Allele: Dmel\rprUAS.cUa
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General Information
Symbol
Dmel\rprUAS.cUa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Unknown
FlyBase ID
FBal0244164
Feature type
allele
Associated gene
Dmel\rpr
Associated Insertion(s)
Carried in Construct
P{UAS-rpr.U}
Also Known As
UAS-rpr, UAS-reaper
Key Links
Transgenic product class
wild_type
(Kondo et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kondo et al., 2006)
Mutagen
in vitro construct
(Kondo et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-rpr.U}
Cytology
Description

UAS regulatory sequences drive expression of rpr.

(Kondo et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
rpr
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal behavior | adult stage, with Scer\GAL4Ir40a.3011, hidUAS.cUa

      (Li et al., 2022)

      abnormal behavior | adult stage, with Scer\GAL4Ir68a.PK, hidUAS.cUa

      (Li et al., 2022)

      abnormal behavior | adult stage, with Scer\GAL4nan.PU, hidUAS.cUa

      (Li et al., 2022)

      abnormal behavior | adult stage, with Scer\GAL4Or42b.PF, hidUAS.cUa

      (Li et al., 2022)

      abnormal cell number | larval stage, with Scer\GAL4GMR9D10, hidUAS.cUa

      (Zhang et al., 2024)

      abnormal feeding behavior | adult stage, with Scer\GAL4Lk.3.6

      (Al-Anzi et al., 2010)

      abnormal feeding behavior | adult stage, with Scer\GAL4Lkr.2

      (Al-Anzi et al., 2010)

      abnormal neuroanatomy | larval stage, with Scer\GAL4per.PK, Scer\GAL80Pdf.PS, hidUAS.cUa

      (Liu et al., 2024)

      abnormal neuroanatomy | larval stage, with Scer\GAL4Tab2-201Y, hidUAS.cUa

      (Liu et al., 2024)

      abnormal size, with Scer\GAL4GMR.PF

      (Zhang et al., 2015)

      abnormal size | adult stage, with Scer\GAL4GMR.PF

      (Yanku et al., 2018)

      abnormal size | adult stage, with Scer\GAL4Mef2.PU

      (Min et al., 2017)

      abnormal taste perception, with Scer\GAL4fuss-MI13731-GAL4

      (Rass et al., 2019)

      decreased cell number | larval stage, with Scer\GAL4GMR9D11, hidUAS.cUa

      (Zhang et al., 2024)

      decreased size | adult stage, with Scer\GAL4GMR.PF

      (Palu et al., 2020)

      decreased size | larval stage, with Scer\GAL4Tab2-201Y, hidUAS.cUa

      (Liu et al., 2024)

      increased cell death, with Scer\GAL4GMR.PU

      (Protzer et al., 2009)

      increased cell death, with Scer\GAL4hs.PU

      (Mehrotra et al., 2008)

      increased cell death | adult stage, with Scer\GAL4ninaE.PT

      (Robin et al., 2019)

      increased cell death | cell autonomous, with Scer\GAL4hs.2sev

      (Yang et al., 2013)

      increased cell death | embryonic stage, with Scer\GAL4elav.PU

      (Shklover et al., 2015)

      increased cell death | larval stage, with Scer\GAL4GMR9D10, hidUAS.cUa

      (Zhang et al., 2024)

      increased cell death | larval stage, with Scer\GAL4GMR9D11, hidUAS.cUa

      (Zhang et al., 2024)

      increased cell death | spermatogenesis, with Scer\GAL4nanos.PU

      (Kong et al., 2023)

      increased cell death | third instar larval stage, with Scer\GAL4GMR.PF

      (Zhang et al., 2015)

      lethal - all die during larval stage, with Scer\GAL4ple.PF

      (Bjordal et al., 2014)

      short lived, with Scer\GAL4GMR21E06

      (Koyama et al., 2021)

      visible, with Scer\GAL4GMR.PF

      (Zhang et al., 2015)

      visible, with Scer\GAL4GMR.PFa

      (Reitman et al., 2015)

      visible, with Scer\GAL4GMR.PU

      (Protzer et al., 2009)

      visible | adult stage, with Scer\GAL4GMR.PF

      (Palu et al., 2020, Yanku et al., 2018)
      Phenotype Manifest In

      adult alary muscle cell | absent, with Scer\GAL4org-1.HN39

      (Schaub et al., 2015)

      adult heart ventral longitudinal muscle cell | absent, with Scer\GAL4org-1.HN39

      (Schaub et al., 2015)

      adult midgut, with Scer\GAL4Mef2.PU

      (Min et al., 2017)

      adult midgut muscle cell, with Scer\GAL4Mef2.PU

      (Min et al., 2017)

      calyx of larval mushroom body | larval stage, with Scer\GAL4Tab2-201Y, hidUAS.cUa

      (Liu et al., 2024)

      embryonic/larval salivary gland, with Scer\GAL4hs.PU

      (Mehrotra et al., 2008)

      eye, with Scer\GAL4GMR.PF

      (Palu et al., 2020, Yanku et al., 2018, Zhang et al., 2015)

      eye, with Scer\GAL4GMR.PFa

      (Reitman et al., 2015)

      eye, with Scer\GAL4GMR.PU

      (Protzer et al., 2009)

      eye disc, with Scer\GAL4hs.PU

      (Mehrotra et al., 2008)

      eye disc | cell autonomous, with Scer\GAL4hs.2sev

      (Yang et al., 2013)

      eye disc | third instar larval stage, with Scer\GAL4GMR.PF

      (Zhang et al., 2015)

      eye photoreceptor cell, with Scer\GAL4ninaE.PT

      (Robin et al., 2019)

      intermediate neuronal progenitor | decreased number | larval stage, with Scer\GAL4GMR9D10, hidUAS.cUa

      (Zhang et al., 2024)

      intermediate neuronal progenitor | decreased number | larval stage, with Scer\GAL4GMR9D11, hidUAS.cUa

      (Zhang et al., 2024)

      larval brain, with Scer\GAL4hs.PU

      (Mehrotra et al., 2008)

      larval s-LNv neuron, with Scer\GAL4per.PK, Scer\GAL80Pdf.PS, hidUAS.cUa

      (Liu et al., 2024)

      larval s-LNv neuron, with Scer\GAL4Tab2-201Y, hidUAS.cUa

      (Liu et al., 2024)

      larval ventral nerve cord | embryonic stage, with Scer\GAL4elav.PU

      (Shklover et al., 2015)

      lch1 neuron, with Scer\GAL4salm-459.2

      (Bates and Whitington, 2007)

      male genitalia | conditional, with Scer\GAL4en.PU, Scer\GAL80ts.αTub84B

      (Kuranaga et al., 2011)

      male germline cell, with Scer\GAL4nanos.PU

      (Kong et al., 2023)

      neuron projection | larval stage, with Scer\GAL4per.PK, Scer\GAL80Pdf.PS, hidUAS.cUa

      (Liu et al., 2024)

      neuron projection | larval stage, with Scer\GAL4Tab2-201Y, hidUAS.cUa

      (Liu et al., 2024)

      nucleus, with Scer\GAL4Ntan1-Mz97

      (Bates and Whitington, 2007)

      nucleus, with Scer\GAL4salm-459.2

      (Bates and Whitington, 2007)

      oenocyte | somatic clone, with Scer\GAL4Ntan1-Mz97

      (Bates and Whitington, 2007)

      oenocyte | somatic clone, with Scer\GAL4salm-459.2

      (Bates and Whitington, 2007)

      retina, with Scer\GAL4GMR.PU

      (Protzer et al., 2009)

      type II neuroblast | decreased number | larval stage, with Scer\GAL4GMR9D11, hidUAS.cUa

      (Zhang et al., 2024)

      type II neuroblast | larval stage, with Scer\GAL4GMR9D10, hidUAS.cUa

      (Zhang et al., 2024)

      wing disc, with Scer\GAL4hs.PU

      (Mehrotra et al., 2008)
      Detailed Description
      Statement
      Reference

      Expression of rprUAS.cUa under the control of Scer\GAL4GMR.PF leads to retinal degeneration (monitored using eye size).

      (Palu et al., 2020)

      Expressing rprUAS.cUa under the control of Scer\GAL4ninaE.PT induces apoptosis (cleaved Dcp1) and degeneration of photoreceptor cells in the adult retina, as compared to controls.

      (Robin et al., 2019)

      Expression of rprUAS.cUa under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) does not lead to ectopic wings compared to controls.

      (Worley et al., 2018)

      Expression of rprUAS.cUa under the control of Scer\GAL4GMR.PF results in a small eye phenotype.

      (Yanku et al., 2018)

      Adulthood-only expression of rprScer\UAS.cUa under the combined control of Scer\GAL4Mef2.PU and Gal80[ts] induces intestinal dilation and muscle degeneration, as compared to controls.

      (Min et al., 2017)

      Expression of rprUAS.cUa under the control of Scer\GAL4elav.PU in the embryonic ventral nerve cord increases the volume of apoptotic particles, compared to controls.

      (Shklover et al., 2015)

      Expression of rprScer\UAS.cUa under the control of Scer\GAL4GMR.PF induces cell death in the eye disc and generates a small-eye phenotype.

      (Zhang et al., 2015)

      Neuronal apoptosis induced by expression of rprScer\UAS.cUa under the control of Scer\GAL4hs.2sev does not spread in the eye disc to non-Scer\GAL4hs.2sev-expressing cells.

      (Yang et al., 2013)

      Flies expressing rprScer\UAS.cUa under the control of Scer\GAL4en.PU during pupal development (but not during embryogenesis, regulated by co-expression of Scer\GAL80ts.αTub84B until the end of this stage) display increased timing of acceleration and speed of genitalia rotation.

      (Kuranaga et al., 2011)

      Expression of rprScer\UAS.cUa under the control of Scer\GAL4GMR.PU results in cell autonomous apoptosis in the developing retina. Adults have small, rough eyes.

      (Protzer et al., 2009)

      Expression of rprScer\UAS.cUa in early third-instar larvae under the control of Scer\GAL4hs.PU causes rapid apoptosis (caspase activation) in mitotically cycling cells of the wing disc, eye disc and brain. In endocycling salivary gland cells expression of rprScer\UAS.cUa also causes rapid and robust cell death in all cells, with complete gland destruction within 30 minutes.

      (Mehrotra et al., 2008)

      Expression of rprScer\UAS.cUa in oenocytes and tracheal and epidermal cells in the dorsal region of the embryo under the control of Scer\GAL4salm-459.2 results in gross morphological defects.

      Expression of rprScer\UAS.cUa in Scer\GAL4salm-459.2 mosaic embryos (i.e. only a subset of cells express Scer\GAL4salm-459.2 and are ablated) results in a reduced number of oenocytes, with those oenocytes present often displaying abnormal nuclear morphology. In some cases the oenocyte nuclei are small and irregular, while in others they are elongated.

      In Scer\GAL4salm-459.2 ; rprScer\UAS.cUa mosaic embryos in which three or fewer oenocyte nuclei are visible exhibit v'ch1 axon mis-projection, either anteriorly or towards the lateral cluster neurons (in 20% of such hemisegments). In 11.4% of the hemisegments, one or more lateral cluster axons misproject ventrally and join the v'ch1 axon.

      Expression of rprScer\UAS.cUa in Scer\GAL4Mz97 mosaic embryos (i.e. only a subset of cells express Scer\GAL4Mz97 and are ablated) results in a reduced number of oenocytes, with those oenocytes present often displaying abnormal nuclear morphology. In some cases the oenocyte nuclei are small and irregular, while in others they are elongated.

      In Scer\GAL4Mz97 ; rprScer\UAS.cUa mosaic embryos in which three or fewer oenocyte nuclei are visible exhibit v'ch1 axon mis-projection, either anteriorly or towards the lateral cluster neurons (in 20% of such hemisegments). In 11.4% of the hemisegments, one or more lateral cluster axons misproject ventrally and join the v'ch1 axon.

      (Bates and Whitington, 2007)

      Deletion of Scer\GAL4Gr66a.PD-expressing cells through expression of rprScer\UAS.cUa in decapitated male flies results in increased courtship of these flies by wild-type males.

      (Lacaille et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Other
      Statement
      Reference

      Scer\GAL4Tk.PS, hidUAS.cUa, rprUAS.cUa has decreased cell number | oogenesis | conditional phenotype

      (Ameku et al., 2018)

      Scer\GAL45-HT2A-PL00052, hidUAS.cUa, rprUAS.cUa has abnormal memory | larval stage | conditional phenotype

      (Huser et al., 2017)

      Scer\GAL4Trhn.PB, hidUAS.cUa, rprUAS.cUa has abnormal memory | larval stage phenotype

      (Huser et al., 2017)

      Scer\GAL4GMR58E02, hidUAS.cUa, rprUAS.cUa has abnormal memory | adult stage | nutrition conditional phenotype

      (Rohwedder et al., 2016)

      Scer\GAL4GMR64H06, hidUAS.cUa, rprUAS.cUa has abnormal memory | adult stage | nutrition conditional phenotype

      (Rohwedder et al., 2016)

      Scer\GAL4EL.eveRC, hidUAS.cUa, rprUAS.cUa has uncoordinated | larval stage phenotype

      (Heckscher et al., 2015)

      Scer\GAL4EL.eveRC, hidUAS.cUa, rprUAS.cUa has abnormal locomotor behavior | larval stage phenotype

      (Heckscher et al., 2015)

      Scer\GAL4Tdc2.PC, Scer\GAL80tsh-md621-GAL80, hidUAS.cUa, rprUAS.cUa has abnormal learning | nutrition conditional phenotype

      (Selcho et al., 2014)

      Scer\GAL4Crz.PU, hidUAS.cZa, rprUAS.cUa has chemical resistant phenotype

      (McClure and Heberlein, 2013)

      Scer\GAL4ppk.PG, hidUAS.cUa, rprUAS.cUa has increased cell death | third instar larval stage phenotype

      (Bhattacharya et al., 2012)

      Scer\GAL4Trhn.PB, hidUAS.cUa, rprUAS.cUa has abnormal locomotor behavior | larval stage | nutrition conditional phenotype

      (Huser et al., 2012)

      Scer\GAL4Trhn.PB, hidUAS.cUa, rprUAS.cUa has abnormal chemosensory behavior | larval stage | nutrition conditional phenotype

      (Huser et al., 2012)

      Scer\GAL4Tdc2.PC, hidUAS.cUa, rprUAS.cUa has abnormal locomotor behavior | larval stage phenotype

      (Selcho et al., 2012)

      Scer\GAL4ppk.1.9, hidUAS.cUa, rprUAS.cUa has abnormal phototaxis phenotype

      (Xiang et al., 2010)
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Females co-expressing rprUAS.cUa and hidUAS.cUa under the control of Scer\GAL4Tk.PS fail to show the expected mating-induced increase in germline stem cells in the germarium, as compared to controls.

      (Ameku et al., 2018)

      Expression of rprUAS.cUa under the control of Scer\GAL4rn-GAL4-5 (with tub-Gal80[ts] restricting expression to third instar larvae) in CtBP87De-10/+ background does not lead to ectopic wings compared to controls.

      (Worley et al., 2018)

      Expression of rprScer\UAS.cUa under the control of Scer\GAL4Hml.PU suppresses the increase in plasmatocytes seen in C1GalTAEY13370 mutant lymph glands. The atypical lymph gland surface and the reduction in hematopoietic stem cells are also suppressed.

      (Fuwa et al., 2015)

      Selective ablation of octopamine/tyramine neurons in the brains through co-expression of hidScer\UAS.cUa and rprScer\UAS.cUa under the control of Scer\GAL4Tdc2.PC and VNC-specific Scer\GAL80tsh-GAL80 does not lead to any deficit in appetitive olfactory learning when fructose is given as a reward. Larvae also exhibit robust appetitive memory when sorbitol is used as a reward, comparable to that of genetic controls. In contrast, the larvae fail to show significant learning when rewarded with arabinose; memory performance is significantly reduced compared with controls. Perception of the odors (amyl acetate and benzaldehyde) is unaffected, and larvae show normal attraction behavior toward arabinose and perform indistinguishably from controls.

      (Selcho et al., 2014)

      Flies co-expressing rprScer\UAS.cUa and WScer\UAS.cZa under the control of Scer\GAL4Crz.PU show a significant reduction in ethanol sedation sensitivity compared to wild type.

      (McClure and Heberlein, 2013)

      hid110 mutants expressing both hidScer\UAS.cUa and rprScer\UAS.cUa in class IV dendritic arborization neurons (under the control of Scer\GAL4ppk.1.9) show markedly decreased white-light-avoidance behavior compared to wild-type and hid110 (bolwig-organ-ablated) mutants.

      (Xiang et al., 2010)

      Co-expression of α4GT1dsRNA.Scer\UAS enhances the cell death in the developing retina caused by expression of rprScer\UAS.cUa under the control of Scer\GAL4GMR.PU.

      Co-expression of α4GT1dsRNA.Scer\UAS.P\T slightly enhances the adult eye phenotype caused by expression of rprScer\UAS.cUa under the control of Scer\GAL4GMR.PU.

      Co-expression of α4GT1EP797 suppresses the increased cell death in the developing retina and the adult eye phenotype caused by expression of rprScer\UAS.cUa under the control of Scer\GAL4GMR.PU.

      (Protzer et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of BacA\p35Scer\UAS.cHa and rprScer\UAS.cUa in the posterior compartment of the wing disc under the control of Scer\GAL4hh-Gal4 induces dramatic tissue overgrowth. This tissue overgrowth is suppressed by NcΔA8/NcΔA8 but is not suppressed by IceΔ2C8/IceΔ2C8.

      (Kondo et al., 2006)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      rprScer\UAS.cUa
      rprUAS.cUa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Unknown
      Secondary FlyBase IDs
        References (91)