Allele Dmel\Spn77Ba^{dsRNA.WIZ.Scer\UAS}

General Information
Symbol	Dmel\Spn77Ba^{dsRNA.WIZ.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0244341
Feature type	allele	Associated gene	Dmel\Spn77Ba

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Tang et al., 2008) in vitro construct - RNAi (Tang et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of two inverted copies of a 551bp fragment corresponding to nucleotides 200-750 of Spn77Ba. (Tang et al., 2008)
Carried in construct	P{UAS-Spn77Ba.IR.WIZ} (Tang et al., 2008, Nam et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (with Spn77Ba^W7), with Scer\GAL4^btl.PS (Tang et al., 2008) lethal \| third instar larval stage, with Scer\GAL4^Act.PU (Tang et al., 2008) visible \| adult stage, with Scer\GAL4^btl.PS (Tang et al., 2008) visible \| larval stage, with Scer\GAL4^Act.PU (Tang et al., 2008) visible \| larval stage, with Scer\GAL4^btl.PS (Tang et al., 2008) visible \| pupal stage, with Scer\GAL4^btl.PS (Tang et al., 2008)
Phenotype Manifest In
	adult tracheal system, with Scer\GAL4^btl.PS (Tang et al., 2008) embryonic/larval tracheal system, with Scer\GAL4^Act.PU (Tang et al., 2008) embryonic/larval tracheal system, with Scer\GAL4^btl.PS (Tang et al., 2008) pupal tracheal system, with Scer\GAL4^btl.PS (Tang et al., 2008) trachea, with Scer\GAL4^da.G32 (Nam et al., 2012)
Detailed Description
	Statement Reference Larvae expressing Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[da.G32] undergo spontaneous tracheal melanization. (Nam et al., 2012) Expression of Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[Act] results in melanisation of the tracheal system in larvae. The phenotype is first visible during the second or third larval instar and increases in intensity until the affected larvae die within a few days before reaching the pupal stage. Expression of Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[btl.PS] results in tracheal melanisation in larvae, pupae and adults. The animals all develop to the prepupal stage. Approximately 35% of prepupae or pupae show severe melanisation of the tracheal system. The remaining 65% are able to develop into adults, of which 96% show tracheal melanisation. 81% of flies expressing Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[btl.PS] are viable at 18[o]C, whereas all animals die before the adult stage if the animals also carry Spn77Ba[W7]/+. The melanisation resulting from expression of Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[btl.PS] is first detected at a low level between tracheal cells and the overlying cuticle. As the amount of melanin increases, it spreads to encompass the epithelial layer occupied by the tracheal cells. Flies expressing Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[c564] have a similar survival rate after microbial infection as control flies. (Tang et al., 2008)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has lethal phenotype, enhanceable by MP1^{Scer\UAS.P\T.T:SS-ea}, Scer\GAL4^btl.PS (Tang et al., 2008)
Suppressed by
Statement Reference Scer\GAL4^Act.PU, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has lethal \| larval stage phenotype, suppressible by MP1^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^Act.PU (Tang et al., 2008) Scer\GAL4^Act.PU, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has lethal \| larval stage phenotype, suppressible by Scer\GAL4^Act.PU/Sp7^{dsRNA.Scer\UAS.WIZ} (Tang et al., 2008) Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has lethal phenotype, suppressible by MP1^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^btl.PS (Tang et al., 2008) Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has lethal phenotype, suppressible by Scer\GAL4^btl.PS/Spn27A^Scer\UAS.cLa (Tang et al., 2008)
NOT suppressed by
Statement Reference Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has lethal phenotype, non-suppressible by Bc¹ (Tang et al., 2008)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^Act.PU, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has tracheal system phenotype, suppressible by MP1^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^Act.PU (Tang et al., 2008) Scer\GAL4^Act.PU, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has tracheal system phenotype, suppressible by Scer\GAL4^Act.PU/Sp7^{dsRNA.Scer\UAS.WIZ} (Tang et al., 2008) Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has adult tracheal system phenotype, suppressible by MP1^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^btl.PS (Tang et al., 2008) Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has adult tracheal system phenotype, suppressible by Scer\GAL4^btl.PS/Spn27A^Scer\UAS.cLa (Tang et al., 2008) Scer\GAL4^da.G32, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has trachea phenotype, suppressible by Hayan¹ (Nam et al., 2012)
NOT suppressed by
Statement Reference Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has adult tracheal system phenotype, non-suppressible by Bc¹ (Tang et al., 2008) Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has adult tracheal system phenotype, non-suppressible by PGRP-SA^seml (Tang et al., 2008) Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has adult tracheal system phenotype, non-suppressible by psh⁵ (Tang et al., 2008) Scer\GAL4^btl.PS, Spn77Ba^{dsRNA.WIZ.Scer\UAS} has adult tracheal system phenotype, non-suppressible by spz⁴ (Tang et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference A Hayan[1] mutant background suppresses the spontaneous tracheal melanization seen when Spn77Ba[dsRNA.WIZ.Scer\UAS] is expressed under the control of Scer\GAL4[da.G32]. (Nam et al., 2012) Bc[1] does not suppress lethality or tracheal melanisation in adults caused by expression of Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[btl.PS]. The lethality and melanisation of the adult tracheal system caused by expression of Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[btl.PS] is suppressed by co-expression of MP1[dsRNA.Scer\UAS.WIZ]. Co-expression of MP1[Scer\UAS.P\T.T:ea] enhances the lethality caused by expression of Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[btl.PS]. Co-expression of Spn27A[Scer\UAS.cLa] suppresses the lethality and tracheal melanisation caused by expression of Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[btl.PS]. The melanisation of the adult tracheal system caused by expression of Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[btl.PS] is not suppressed by PGRP-SA[seml], psh[5] or spz[4]. The degree of melanisation seen in larvae expressing Spn77Ba[dsRNA.WIZ.Scer\UAS] under the control of Scer\GAL4[btl.PS] increases with greater microbial exposure. (Tang et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Spn77Ba^{dsRNA.WIZ.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Nam et al., 2012, EMBO J. 31(5): 1253--1265 Genetic evidence of a redox-dependent systemic wound response via Hayan Protease-Phenoloxidase system in Drosophila. [FBrf0217593] Tang et al., 2008, Dev. Cell 15(4): 617--626 A serpin that regulates immune melanization in the respiratory system of Drosophila. [FBrf0206001]

Allele Dmel\Spn77BadsRNA.WIZ.Scer\UAS

Allele Dmel\Spn77Ba^{dsRNA.WIZ.Scer\UAS}