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General Information
Symbol
Dmel\hidmiRNA.RHG.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0244380
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-miRHG, UAS-miRGH, UAS-RHG miRNA
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of three microRNAs, directed against rpr, hid and grim respectively.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The triple expression of grimmiRNA.RHG.UAS, hidmiRNA.RHG.UAS and rprmiRNA.RHG.UAS under the control of Scer\GAL4C10 induces significantly smaller wings, with significantly fewer cells, although slightly bigger, as compared to controls. Likewise, the legs are significantly smaller than controls (femur and tarsal segment 1, but not tibia). Wing discs show a significant decrease in apoptosis (detected by PARP:VENUS signal and TUNEL assay), as compared to controls.

The triple expression under the control of Scer\GAL4ap-md544 leads to opaque and curly wings, which indicate apoptosis and growth inhibition during development, respectively.

The eye defect caused by WGMR.PU is effectively rescued by expression of P{UAS-RHG.miRNA} (carries WmiRNA.RHG.Scer\UAS, grimmiRNA.RHG.Scer\UAS and rprmiRNA.RHG.Scer\UAS) under the control of Scer\GAL4GMR.PF.

Mushroom body neuroblasts are seen in the brains of young adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) under the control of Scer\GAL4wor.PA, although their persistence is only transient (mushroom body neuroblasts are not seen in wild-type adults).

Neuroblasts are detected in the mushroom body of 2 week old and even in 1 month old adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) under the control of Scer\GAL4wor.PA in a foxo25 background (mushroom body neuroblasts are not seen in wild-type adults and are not seen at these late time points in adults expressing the P{UAS-RHG.miRNA} transgene under the control of Scer\GAL4wor.PA in a wild-type background or in foxo25 single mutant adults). Some of the 2 week old mutant mushroom body neuroblasts are large in size and generate many new progeny. There is a strong correlation between mushroom body neuroblast cell size and progeny number in 2 week old mutant adults, but not in younger mutant adults. In 1 month old mutant adults, some of the progeny of these neuroblasts show normal axon projections through the mushroom body pedunculus and some mistarget, bifurcating prematurely and projecting anterior to the pedunculus.

Neuroblasts are detected in the mushroom body of 1 month old adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) and Atg1K38Q.Scer\UAS under the control of Scer\GAL4wor.PA (mushroom body neuroblasts are not seen in wild-type adults).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
WmiRNA.RHG.Scer\UAS
hidmiRNA.RHG.Scer\UAS
hidmiRNA.RHG.UAS
Name Synonyms
Secondary FlyBase IDs
    References (10)