Allele Dmel\dream⁴

General Information
Symbol	Dmel\dream⁴	Species	D. melanogaster
Name		FlyBase ID	FBal0244459
Feature type	allele	Associated gene	Dmel\dream
Also Known As	strica⁴

Allele class	amorphic allele - molecular evidence
Mutagen	Delta2-3
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - molecular evidence (Baum et al., 2007)
Mutagen	Delta2-3 (Baum et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{XP}dream^d06491 (Baum et al., 2007)
Nature of the lesion	Statement Reference Imprecise excision of the progenitor insertion, resulting in a 2.63kb deletion which removes genomic sequence from 355bp upstream of the original insertion site, the entire 527 amino acid dream coding region and all of the transcribed region of dream except the final 70bp. (Baum et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Baum et al., 2007) viable (Baum et al., 2007)
Phenotype Manifest In

Detailed Description
	Statement Reference dream[4] homozygotes exhibit wild-type apoptosis of vCrz neurons, with no viable vCrz neurons detected at 7 hours after puparium formation. (Lee et al., 2011) No abnormal mid-stage egg chambers are seen in the ovaries of homozygous or dream[4]/Df(2R)nap9 females. (Baum et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference dream⁴ has cell death defective phenotype, enhanceable by Nc^I24 (Lee et al., 2011)
NOT Enhanced by
Statement Reference Nc^I24, dream⁴ has cell death defective phenotype, non-enhanceable by Dredd^B118 (Lee et al., 2011)
NOT suppressed by
Statement Reference Nc^I24, dream⁴ has cell death defective phenotype, non-suppressible by Dredd^B118 (Lee et al., 2011)
Enhancer of
Statement Reference dream⁴ is an enhancer of cell death defective phenotype of Nc^I24 (Lee et al., 2011) dream⁴ is an enhancer of cell death defective phenotype of Nc^I24/Nc⁵¹ (Lee et al., 2011)
Other
Statement Reference Nc^I24, dream⁴ has female fertile \| germline clone phenotype (Baum et al., 2007) Nc^I29, dream⁴ has female fertile \| germline clone phenotype (Baum et al., 2007)
Phenotype Manifest In
Enhanced by
Statement Reference dream⁴ has neuron phenotype, enhanceable by Nc^I24 (Lee et al., 2011)
NOT Enhanced by
Statement Reference Nc^I24, dream⁴ has neuron phenotype, non-enhanceable by Dredd^B118 (Lee et al., 2011)
NOT suppressed by
Statement Reference Nc^I24, dream⁴ has neuron phenotype, non-suppressible by Dredd^B118 (Lee et al., 2011)
Enhancer of
Statement Reference dream⁴ is an enhancer of neuron phenotype of Nc^I24 (Lee et al., 2011)
Other
Statement Reference Nc^I24, dream⁴ has egg chamber \| germline clone phenotype (Baum et al., 2007) Nc^I29, dream⁴ has egg chamber \| germline clone phenotype (Baum et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference Nearly all vCrz neurons are present at 7 hours after puparium formation in dream[4]; Nc[I24] double mutants. Such an abnormal programmed cell death phenotype is significantly more severe than in either single mutant. No surviving vCrz neurons are found at 7 hours after puparium formation in dream[4]; Nc[I24]/+ mutants, suggesting that heterozygosity of Nc is sufficient to compensate for the lack of dream function. Triple mutants of Dredd[B118]; dream[4]; Nc[I24] display results comparable to dream[4]; Nc[I24] double mutants, i.e. all vCrz neurons are still present having not undergone apoptosis by 7 hours after puparium formation. (Lee et al., 2011) dream[4] ; Nc[I24]/Nc[I29] Ice[Δ1] triple mutant larvae show high levels of TUNEL staining at -4 to -1 hours relative to puparium formation, suggesting that they are undergoing cell death. (Denton et al., 2009) Females containing dream[4] ; Nc[I24] double homozygous germline clones are fertile, but their ovaries contain 31% of mid-stage egg chambers with missing follicle cells and large surviving nurse cells. There is also an increase in mid-stage egg chambers containing condensed nurse cell nuclei but lacking follicle cells. 16% of stage 14 egg chambers contain persisting nurse cell nuclei. Females containing dream[4] ; Nc[I29] double homozygous germline clones are fertile, but their ovaries contain 46% of mid-stage egg chambers with missing follicle cells and large surviving nurse cells. Many of these egg chambers contain excessive numbers of nurse cells. There is also an increase in mid-stage egg chambers containing condensed nurse cell nuclei but lacking follicle cells. 21% of stage 14 egg chambers contain persisting nurse cell nuclei. No significant defects is seen in the egg chambers of females containing dream[2]/dream[4] ; Nc[I29] double homozygous germline clones. Dredd[B118] dream[4] double mutant females do not show any defects during oogenesis. (Baum et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	dream⁴ strica⁴ (Denton et al., 2009, Lee et al., 2011, Florentin and Arama, 2012, Baum et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Florentin and Arama, 2012, J. Cell Biol. 196(4): 513--527 Caspase levels and execution efficiencies determine the apoptotic potential of the cell. [FBrf0217486] Lee et al., 2011, J. Comp. Neurol. 519(1): 34--48 Drosophila caspases involved in developmentally regulated programmed cell death of peptidergic neurons during early metamorphosis. [FBrf0212368] Denton et al., 2009, Curr. Biol. 19(20): 1741--1746 Autophagy, not apoptosis, is essential for midgut cell death in Drosophila. [FBrf0209243] Baum et al., 2007, Cell Death Differ. 14(8): 1508--1517 The Drosophila caspases Strica and Dronc function redundantly in programmed cell death during oogenesis. [FBrf0201400]

Allele Dmel\dream4

Allele Dmel\dream⁴