FlyBase Allele Report: Dmel\Ube3a[UAS.cWa]

General Information

Symbol

Dmel\Ube3a^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Wu

FlyBase ID

FBal0244599

Feature type

allele

Associated gene

Dmel\Ube3a

Associated Insertion(s)

Carried in Construct

P{UAS-Ube3a.W}

Key Links

Transgenic product class

cDNA

(Wu et al., 2008)

wild_type

(Wu et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Wu et al., 2008)

wild_type

(Wu et al., 2008)

Mutagen

in vitro construct

(Wu et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Ube3a.W}

Cytology

Description

UASt regulatory sequences drive expression of an Ube3a cDNA.

(Wu et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ube3a

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intellectual disability

CEA

(Chakraborty et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | adult stage, with Scer\GAL4^elav.PU

(Chakraborty et al., 2015)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PLu

(Wu et al., 2008)

abnormal size, with Scer\GAL4^GMR.PU

(Lee et al., 2014)

lethal, with Scer\GAL4^αTub84B.PL

(Wu et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Wu et al., 2008)

eye, with Scer\GAL4^GMR.PU

(Lee et al., 2014)

retina, with Scer\GAL4^GMR.PF

(Wu et al., 2008)

wing, with Scer\GAL4^Bx-MS1096

(Wu et al., 2008)

Detailed Description

Statement

Reference

Adults expressing Ube3a^Scer\UAS.cWa under the control of Scer\GAL4^elav.PU show learning defects (significantly reduced performance in a aversive phototaxis suppression assay compared to controls).

(Chakraborty et al., 2015)

Scer\GAL4^GMR.PU-mediated expression of Ube3a^Scer\UAS.cWa causes a degenerative eye phenotype and a reduction in eye size.

(Lee et al., 2014)

Flies expressing Ube3a^Scer\UAS.cWa under the control of Scer\GAL4^elav.PLu show reduced climbing ability, and this defect is more severe in older flies.

(Wu et al., 2008)

Expression of Ube3a^Scer\UAS.cWa in the eye, under the control of Scer\GAL4^GMR.PF, results in a rough-eye phenotype and severely disrupted retinal morphology. Expression in the wing, under the control of Scer\GAL4^Bx-MS1096, results in a curly-wing phenotype. Ubiquitous expression, under the control of Scer\GAL4^αTub84B.PL, results in lethality.

(Wu et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Ube3a^UAS.cWa has abnormal size phenotype, suppressible by Rpn10^CTH.UAS, Scer\GAL4^GMR.PU

(Lee et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Ube3a^UAS.cWa has eye phenotype, suppressible by Rpn10^CTH.UAS, Scer\GAL4^GMR.PU

(Lee et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rpn10^CTH.Scer\UAS completely suppresses the degenerative eye phenotype caused by Scer\GAL4^GMR.PU-mediated expression of Ube3a^Scer\UAS.cWa.

(Lee et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/Ube3a^UAS.cWa rescues Ube3a^unspecified

(Chakraborty et al., 2015)

Comments

Expression of Ube3a^Scer\UAS.cWa under the control of Scer\GAL4^elav.PU rescues the learning defects (reduced performance in a aversive phototaxis suppression assay) of adults which have a maternally inherited null mutation of Ube3a.

(Chakraborty et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ube3a^Scer\UAS.cWa

Ube3a^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Wu

Secondary FlyBase IDs

References (4)

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