Allele Dmel\Ras85D^KP

General Information
Symbol	Dmel\Ras85D^KP	Species	D. melanogaster
Name		FlyBase ID	FBal0244615
Feature type	allele	Associated gene	Dmel\Ras85D

Allele class
Mutagen	spontaneous
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	spontaneous (Wu et al., 2009)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion of a 1165 bp fragment of a P{KP} element in the second exon of Ras85D, located 88 bp upstream of the start codon. (Wu et al., 2009)
Caused by insertion	P{KP}Ras85D^KP (Wu et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell size (Wu et al., 2009) female semi-sterile (Wu et al., 2009) increased cell death \| third instar larval stage (Wu et al., 2009) partially lethal - majority die \| pupal stage (Wu et al., 2009) small body (Wu et al., 2009)
Phenotype Manifest In
	eye (Wu et al., 2009) eye disc \| third instar larval stage (Wu et al., 2009) ommatidium (Wu et al., 2009) photoreceptor cell (Wu et al., 2009) wing (Wu et al., 2009)
Detailed Description
	Statement Reference Ras85D[KP] and wild-type larvae are comparable in body size. However, mutant adults show significantly reduced body weight. Homozygous mutants show a 23.5% reduction in wing size, and decreased wing cell size. Ras85D[KP] mutant adults have a rough-eye phenotype and the eye is reduced in size. Neuronal differentiation in third instar larval eye discs is observed, however adult eyes show largely disorganised ommatidia. The full complement of photoreceptors are seen, but many ommatidia contain fewer photoreceptor cells. There is substantially increased levels of cell death in third instar larval eye discs. (Wu et al., 2009)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference BacA\p35^GMR.PH, Ras85D^KP has increased cell death phenotype, suppressible by hep^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) BacA\p35^GMR.PH, Ras85D^KP has visible phenotype, suppressible by hep^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) BacA\p35^GMR.PH, Ras85D^KP has visible phenotype, suppressible by Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has increased cell death phenotype, suppressible \| partially by W⁰⁵⁰¹⁴/Df(3L)H99 (Wu et al., 2009) Ras85D^KP has visible phenotype, suppressible \| partially by BacA\p35^GMR.PH (Wu et al., 2009) Ras85D^KP has visible phenotype, suppressible \| partially by bsk^DN.Scer\UAS/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has visible phenotype, suppressible \| partially by egr^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has visible phenotype, suppressible \| partially by hep^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has visible phenotype, suppressible \| partially by Tak1^NIG.1388R/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has visible phenotype, suppressible \| partially by Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has visible phenotype, suppressible \| partially by W⁰⁵⁰¹⁴/Df(3L)H99 (Wu et al., 2009) Ras85D^KP has visible phenotype, suppressible \| partially by wgn^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) W⁰⁵⁰¹⁴/Df(3L)H99, Ras85D^KP has visible phenotype, suppressible by hep^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009)
NOT suppressed by
Statement Reference Ras85D^KP has visible phenotype, non-suppressible by Akt1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has visible phenotype, non-suppressible by Buffy^Scer\UAS.cQa/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has visible phenotype, non-suppressible by Scer\GAL4^GMR.PF/Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Wu et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference BacA\p35^GMR.PH, Ras85D^KP has eye phenotype, suppressible by hep^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) BacA\p35^GMR.PH, Ras85D^KP has eye phenotype, suppressible by Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has eye phenotype, suppressible \| partially by BacA\p35^GMR.PH (Wu et al., 2009) Ras85D^KP has eye phenotype, suppressible \| partially by bsk^DN.Scer\UAS/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has eye phenotype, suppressible \| partially by egr^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has eye phenotype, suppressible \| partially by hep^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has eye phenotype, suppressible \| partially by Tak1^NIG.1388R/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has eye phenotype, suppressible \| partially by Traf4^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has eye phenotype, suppressible \| partially by W⁰⁵⁰¹⁴/Df(3L)H99 (Wu et al., 2009) Ras85D^KP has eye phenotype, suppressible \| partially by wgn^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009) W⁰⁵⁰¹⁴/Df(3L)H99, Ras85D^KP has eye phenotype, suppressible by hep^{dsRNA.Scer\UAS}/Scer\GAL4^GMR.PF (Wu et al., 2009)
NOT suppressed by
Statement Reference Ras85D^KP has eye phenotype, non-suppressible by Akt1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has eye phenotype, non-suppressible by Buffy^Scer\UAS.cQa/Scer\GAL4^GMR.PF (Wu et al., 2009) Ras85D^KP has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF/Pi3K92E^{Scer\UAS.T:Hsap\MYC} (Wu et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Df(3L)H99/W[05014] partially suppresses the cell death phenotype of Ras85D[KP] mutant flies. The eye phenotype in Ras85D[KP] mutant flies is not suppressed by ectopic expression of Pi3K92E[Scer\UAS.T:Hsap\MYC], Akt1[Scer\UAS.T:Ivir\HA1] or Buffy[Scer\UAS.cQa] under the control of Scer\GAL4[GMR.PF]. The eye phenotype in Ras85D[KP] mutant flies is partially suppressed by ectopic expression of Traf4[dsRNA.Scer\UAS], Tak1[dsRNA.Scer\UAS], hep[dsRNA.Scer\UAS], bsk[DN.Scer\UAS], egr[IR.Scer\UAS], or wgn[dsRNA.Scer\UAS] in the eye under the control of Scer\GAL4[GMR.PF]. Expression of hep[dsRNA.Scer\UAS] (under the control of Scer\GAL4[GMR.PF]) in flies carrying Df(3L)H99/W[05014] in a Ras85D[KP] mutant background strongly suppresses the eye phenotype. (Wu et al., 2009)
Xenogenetic Interactions
Statement Reference Expression of BacA\p35[GMR.PH] in a Ras85D[KP] mutant background only partially suppresses increased cell death. Expression of Traf4[dsRNA.Scer\UAS] (under the control of Scer\GAL4[GMR.PF]) and BacA\p35[GMR.PH] in a Ras85D[KP] mutant background strongly suppresses the eye phenotype. Expression of hep[dsRNA.Scer\UAS] (under the control of Scer\GAL4[GMR.PF]) and BacA\p35[GMR.PH] in a Ras85D[KP] mutant background strongly suppresses the eye phenotype, and much reduced levels of cell death are observed. (Wu et al., 2009)
Complementation & Rescue Data
Fails to complement	Ras85D^e1B (Wu et al., 2009) Ras85D^e2F (Wu et al., 2009) Ras85D^ΔC17b (Wu et al., 2009)
Rescued by	Ras85D^KP is rescued by Scer\GAL4^Act.PU/Ras85D^Scer\UAS.cKa (Wu et al., 2009)
Comments	Expression of Ras85D[Scer\UAS.cKa] under the control of Scer\GAL4[Act] in Ras85D[KP] mutant animals fully rescues the mutant eye phenotype. (Wu et al., 2009)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Ras85D^KP ras^KP (Wu et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Wu et al., 2009, BMC Dev. Biol. 9: 53 Ras promotes cell survival by antagonizing both JNK and Hid signals in the Drosophila eye. [FBrf0209242]

Allele Dmel\Ras85DKP

Allele Dmel\Ras85D^KP